[1] Li Jin Bu Diaodiao Wang Mei Ding Maopeng,.Ｒapid Determination of Pesticide Ｒesidue in Jinyinhua Soft Capsule Extract by SPE Purification Combined with GC － MS [J]. Modern Traditional Chinese Medicine, 2017, (03): 75-78. [doi: 10.13424/j.cnki.mtcm.2017.03.030]
Ｒapid Determination of Pesticide Ｒesidue in Jinyinhua Soft Capsule Extract by SPE Purification Combined with GC － MS
- Article number:
- 1672－0571(2017)03－0075－04
- Keywords:
- GC － MS; SPE; pesticide residue; soft capsule extract
- ZTFLH:
- Ｒ284.1
- Code:
- A
- Abstract:
- Objective: To control the quality of Jinyinhua Soft Capsule Extract and to rapidly determine the pesticide residue． Method: The study was to establish a SPE purification method combined with GC － MS to determine the content of pesticide residues of organophosphorus and pyrethroid in the extract． The samples were firstly extracted by acetone and water mixed with petroleum ether and acetone，dehydrated by anhydrous sodium sulfate and purified by Florisil SPE col- umn to lastly determine the residues in GC － MS instrument． Ｒesults: The results showed that the samples ranged from 0． 3mg /kg to 3mg /kg，the average recovery rate was between 70% and 120% determined by external standard method; the limit of detection ( LOD) was between 0． 01mg /kg and 0． 05mg /kg，and the relative standard deviation ( ＲSD) was ranged from 1． 96% to 10． 66% ． Conclusion: The designed method is rapid，easy － operated and relatively simple with small interference matrix and good purification effect，which can meet the needs of detecting a variety of pesticide residues in soft capsule extract．
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Memo
收稿日期:2016－09－12编辑:文颖娟*
基金项目:国家自然科学基金(81403101);陕西省教育厅重点实验室项目(11JS036);陕西省教育厅项目(15JS024)