[1] Gao Xuliang Wang Qilong,.Experimental study on Inhibitory Effects ofPaeoniflorin on Oxidative iStress n ＲeducingPostoperative IntraperitonealAdhesions in Ｒats [J]. Modern Traditional Chinese Medicine, 2018, (01): 89-92. [doi: 10．13424/j．cnki．mtcm．2018．01．031]
Experimental study on Inhibitory Effects ofPaeoniflorin on Oxidative iStress n ＲeducingPostoperative IntraperitonealAdhesions in Ｒats
- Article number:
- 1672－0571(2018)01－089－04
- Keywords:
- intraperitoneal adhesion; paeoniflorin; chitosan
- ZTFLH:
- Ｒ28
- Code:
- A
- Abstract:
- In order to find a solution to postoperative intraperitoneal adhesion，we use paeoniflorin an- tioxidant mechanism to explore the effect of reducing postoperative intraperitoneal adhesion in animal models． Method: Thirty － two healthy male Sprague － Dawley rats were randomly divided into 4 groups: blank control group，control group，chitosan positive control group and paeoniflorin group，with 8 rats in each group． Expect for the blank control group，postoperative intraperitoneal adhesion models were constructed in other groups and all animals were sacrificed on the 8th day after surgery． intraperitoneal adhesions were evaluated visually and scored． The content of fibrotic AGE，Mas- son＇s staining and the activity of SOD － 2 and NO were detected． The expression of SOD － 2 and Collenge I were further analyzed by Western blotting． Ｒesult: On day 8 postoperatively，the formation of peritoneal adhesions after paeoniflorin treatment was evaluated． The results showed that the adhesion score was significantly lower than that of the control group ( P ＜ 0． 50) ，the difference was statistically significant ( P ＜ 0． 50) ． Paeoniflorin treatment reduced the adhesion tissue fi- bers，AGE，Masson＇s staining，collagen 1 expression was also significantly lower than the control group ( P ＜ 0． 50) ，and the adhesion tissue SOD － 2 and NO expression was significantly higher than the control group，with statistical difference ( P ＜ 0． 05) ． Conclusion: Paeoniflorin can reduce the formation of postoperative intraperitoneal adhesion by promoting the antioxidant mechanism of tissues． It is more effective than the chitosan used to prevent postoperative adhesion and has the potential to prevent abdominal adhesion after operation．
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Memo
收稿日期: 2017 － 04 － 14 编辑:文颖娟