[1] Fan Shu Guo Dongyan Zheng Bei Pan Xiaoxia Xie Feng,.Experimental Study on the Total Flavonoids of Malus Hupehensis in Two Acute Liver Injury Mice Models [J]. Modern Traditional Chinese Medicine, 2018, (05): 116-120. [doi: 10．13424/j．cnki．mtcm．2018．05．039]
Experimental Study on the Total Flavonoids of Malus
Hupehensis in Two Acute Liver Injury Mice Models
- Article number:
- 1672－0571(2018)05－0116－05
- ZTFLH:
- Ｒ28
- Code:
- A
- Abstract:
- To study the protective effect of total flavonoids of malus hupehensis on acute chemical liver injury and acute alcoholic liver injury in mice． Method: 120 Kunming mice were randomly divided into two different liver injury models． Each animal model was divided into 6 groups: blank con- trol group，model group，positive control group (biphenyldicarboxylate 125 mg·kg －1)，high，medi- um and low dose groups of total flavonoids of malus hupehensis(1． 68，0． 84，0． 42g·kg －1)，10 in each group，male and female were separately fed and administered by intragastric administration． As for the mice with acute chemical liver injury，the blank control group and the model group were intragastri-cally administered with the same volume of normal saline． The positive group was given bifendate． The treatment group was given different doses of total flavonoidsof malus hupehensis for 7 days and the last administration for 2 hours． Thereafter，0． 1 mL of ? 10 g －1，0． 1% CCl4 olive oil solution was intrap- eritoneally injected． As for the mice with acute alcoholic liver injury，except for the blank control group，the other groups were given 56° Beijing Erguotou (10mg·kg －1) every morning，and the total flavonoids of malus hupehensis were treated in the afternoon for 7 days． All the mice in the experimental group were taken 8 hours after the last administration，and the liver index，serum alanine aminotrans- ferase (ALT)，aspartate aminotransferase (AST) content，and HE staining for liver tissue pathological changes were detected． Ｒesult: In the two models of acute liver injury，compared with the model group， different doses of total flavonoids of malus hupehensis can reduce the levels of ALT and AST in the ser- um of mice with liver injury (P ＜0． 01)，and improve pathological damage of liver in mice． Conclusion: Total flavonoids of malus hupehensis have obvious protective effects on acute liver injury induced by CCL4 and alcohol in mice．
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Memo
收稿日期:2018 －04 －09 编辑:文颖娟＊
＊ ＊
基金项目:陕西省科技厅项目(2016SF － 367);陕西省中医药管理局项目(15 － ZY002);陕西省教育厅项目
(16JK1218);陕西省中药基础与新药研究重点实验室开放基金 (2017KF03);陕西省教育厅项目
(15JF015)
通讯作者:范妤(1977 － )，女，副教授，从事中医药防治消化系统疾病机制的研究。E － mail:806919125@ qq． Com