[1] Xu Pengchong Niu Danqing Zhang Jingming,.Effects of Zuogui and Yougui Pills on Learning and Memory and CREB Protein Expression in Hippocampus of Rats with Kidney Deficiency [J]. Modern Traditional Chinese Medicine, 2019, (01): 88-91. [doi: 10.13424/j.cnki.mtcm.2019.01.030]
Effects of Zuogui and Yougui Pills on Learning and Memory and CREB Protein Expression in Hippocampus of Rats with Kidney Deficiency
- Article number:
- 1672-0571(2019)01-0088-04
- Keywords:
- kidney deficiency; learning and memory; CREB; Zuogui Pill; Yougui Pill
- ZTFLH:
- R392
- Code:
- A
- Abstract:
- To observe the effects of Zuogui Pill and Yougui Pill on learning and memory and the expression of cyclic adenosine phosphate response element binding protein ( CREB) in hippocampus of rats with kidney deficiency, and to further clarify the targets of Zuogui Pill and Yougui Pill in the treatment of learning and memory impairment of kidney deficiency. Method The rat model of kidney deficiency was constructed by the method of “ cat scaring mouse” , the intimidation of the offspring rats was continued, and Zuogui pill and Yougui pill were used for intervention. The learning and memory ability of rats was tested by Morris water maze, and the protein expression level of CREB in hippocampus was determined by enzyme - linked immunosorbent assay. Result ( 1) Learning and memory of offspring rats in each group: in the navigation test, compared with blank group, the escape latency and total distance of kidney deficiency model group were significantly prolonged ( P <0. 05) ; compared with kidneydeficiency model group, the escape latency and total distance of Zuogui Pill and Yougui Pill group were significantly shortened ( P <0. 05) . ( 2) CREB Concentration by enzyme - linked immunosorbent assay: Compared with blank group, CREB protein concentration of kidney deficiency model group were significantly decreased( P <0. 05) . Compared with the kidney deficiency model group, the content of CREB protein in Zuogui Pill and Yougui Pill groups was significantly increased ( P <0. 05) . Conclusion Zuogui Pill and Yougui Pill may improve the learning and memory ability of rats with kidney deficiency by regulating the expression of CREB protein in hippocampus.
References:
[1]薛昶,孙理军,郝蕊. 肾虚体质大鼠生理指标变化的实验研究[J]. 陕西中医,2008,29(4):505 - 506.
[2] Hayward P. Presenilin dysfunction leads to memory andplasticity defects[J]. Lancet Neurology, 2004,3(6):327.
[3] Yang BH, Son HKim SH. Phosphorylation of ERK andCREB in cultured hippocampal neurons after haloperidoland risperidone administration [ J]. Psychiatry & ClinicalNeurosciences,2004,58(3): 262 - 267.
[4]施新猷. 现代医学实验动物学[ M]. 北京:人民军医出版社,2000:332 - 335.
[5]彭艳,白雪,杨思进,等. 加味益气聪明汤对 VD 大鼠行为学变化及海马神经元突触结构的影响[ J]. 陕西中医药大学学报,2017,40(4):105 - 109 + 113.
[6]牛文民,刘智斌,王渊. 嗅三针干预对 AD 模型大鼠学习记忆及大脑边缘叶突触素蛋白含量的影响[ J]. 现代中医药,2017,37(6):126 - 128.
[7]王涛,康涛,雷琦,等. 银杏叶提取物注射液联合醒脑静注射液治疗阿尔茨海默病 70 例[ J]. 陕西中医药大学学报,2018,41(2):30 - 33 + 51.
[8]孙理军. 中医体质学说应用述要[ J]. 山西中医学院学报,2002,3(2):54 - 56.
[9]孙理军,张登本. 中医体质的基本内涵[J]. 天津中医学院学报,2002,21(3):6 - 8.
[10] WugY, Deisseroth K, Tsien RW. Activity - dependent
CREB phosphorylation: Convergence of a fast, sensitive
calmodulin kinase pathway and a slow, less sensitive mito
gen - activated[J]. Proc NatlAcad Sci USA,2001,98(5):2808 - 2813.
[11]Takemori H, Kajimura J, Okamoto M. TORC - SIK cas-cade regulates CREB activity through the basic leucine zip-per domain[J]. FEBS J,2007, 274(13): 3202 - 3209.
[12]Sweatt JD. Mitogen - activated protein kinases in synaptic
plasticity and memory[ J]. Curr Opin Neurobiol,2004,14(3) : 311 - 317.
[13]Kida S,Josselyn SA,Pena de Ortiz S,etal. CREB requiredfor the stability of new and reactivated fear memories[ J].Nature Neurosci,2002,5(4) : 348 - 355.
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陕西省教育厅重点实验室科研计划项目(16JS027)