• 陕西省优秀期刊
  • 主办：陕西中医药大学

[1] SUN Yuan, TIAN Xiu - xiu, DING Jiang - sheng,.Determination of Ginsenoside Rg3 in Different Parts of Panax Notoginseng [J]. Modern Traditional Chinese Medicine, 2021, (02): 29-34. [doi: 10.13424/j.cnki.mtct.2021.02.006]
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Determination of Ginsenoside Rg3 in Different Parts of Panax Notoginseng

Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN] Volumes & Issues: 2021 02 pagination: 29-34 Column: Publication date: 2023-11-20
Article number:
1672-0571(2021)02-0029-06
Author(s):
SUN Yuan 1; 2; 3 ;  TIAN Xiu - xiu 1; 2; 3 ;  DING Jiang - sheng 1; 2; 3
1. Yunnan pharmaceutical research institute, Kunming 650111, China;
2. Innovation and R & D center of Yunnan Baiyao group, Kunming 650111, China;
3. Key laboratory of new drug creation enterprises of traditional Chinese medicine and ethnic medicine in Yunnan province, Kunming 650111, China
Keywords:
Panax notoginseng;  Content determination;  20 (S) - Ginsenoside Rg3;  20 (R) - Ginsenoside Rg3
ZTFLH:
R282.6
DOI:
10.13424/j.cnki.mtct.2021.02.006
Code:
A
Abstract:
Objective To establish a method for the determination of 20 ( s) - Ginsenoside Rg3 and 20 ( R) - Ginsenoside Rg3 in Panax notoginseng, and to evaluate the content of two types of Ginsenoside Rg3 in Panax notoginseng extracts from different medicinal parts and processing methods. Methods HPLC was used to determine the content. The chromatographic conditions were as follows: ecosil 120 -5 - c18 - sh column ( 4. 6 × 250mm, 5μm) ; The mobile phase was acetonitrile - isopropanol - water ( 47: 3: 50) ; the flow rate was 1. 0ml· min -1 ; the detection wavelength was 203nm; the injection volume was 10 μ L; the column temperature was 20 ℃; the contents of 20 ( s) - Ginsenoside Rg3 and 20 ( R) - Ginsenoside Rg3 in different medicinal parts and processing methods of Panax notoginseng extract were determined. Results the linear range of 20 ( s) - Ginsenoside Rg3 was 14. 0 ~ 279. 6 μ g · ml-1 ( r 2= 1. 0000) .The average recovery was 100. 56%, RSD 2. 46%. The linear range of 20 ( R) - Ginsenoside Rg3 was 14. 1 ~ 282. 0μ g · ml-1 ( R 2= 0. 9999) . The average recovery was 101. 51%, RSD 2. 99%. Conclusion The content of 20 ( s) -Ginsenoside Rg3 in cooked Panax notoginseng increased significantly.

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Memo

基金项目:云南省科技厅“重大科技专项(生物医药)”(2018ZF013)
作者简介:孙媛,高级工程师?E-mail:stary1982@163.com

Last Update: 2021-03-26
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