[1] HAN Sirong QU Jie YANG Jingfeng YANG Jun TAN Yingying LI Xiaohui CHEN Liming,.Effects of Didang Qigui Decoction onBlood glucose, IRS-1 and GSK-3β in Liver Tissue of Type 2 Diabetic Rats [J]. Modern Traditional Chinese Medicine, 2022, 1(03): 49-55. [doi: 10.13424/j.cnki.mtcm.2022.03.009]
Effects of Didang Qigui Decoction onBlood glucose, IRS-1 and GSK-3β in Liver Tissue of Type 2 Diabetic Rats
- Article number:
- 1672-0571(2022)03-0049-07
- Keywords:
- Key words：Didang Qigui decoction; Type 2 diabetes; Liver; IRS-1; GSK-3β
- ZTFLH:
- R255.4
- Code:
- A
- Abstract:
- Abstract：Objective To study the effects of Didang Qigui Decoction on blood glucose，insulin receptor substrate-1 (IRS-1) and glycogen synthase kinase3β（GSK-3β）in liver tissue of type 2 diabetic rats (T2DM).Methods Type 2 diabetes model rats were established by high sugar and high fat diet combined with intraperitoneal injection of small dose of streptozotocin.They were randomly divided into model group (model group)，Didang Qigui Decoction high dose group (DQG group)，Didang Qigui Decoction constant group (DQG group)，metformin group (Met group)，Didang Qigui Decoction plus metformin group (DQZX group).After 6 weeks of intragastric administration，fasting blood glucose (FBG) and glycosylated hemoglobin (HbA1c) of rats in each group were detected.At the same time，HE staining was used to observe the tissue morphology of pancreas，PAS staining was used to observe the synthesis of liver glycogen，and immunohistochemical method was used to detect IRS-1 and GSK-3β protein expression in the liver of type 2 diabetic rats.Results After 6 weeks of drug intervention，compared with model group，FBG and HbA1c in DQZ group and DQZX group decreased in varying degrees (P＜0.05 or P＜0.01)，the expression of IRS-1 in liver tissue increased (P<0.01)，and the expression of GSK-3β was decreased (P＜0.05 or P＜0.01).Conclusion Didang Qigui decoction may up regulate the expression of IRS-1and down regulate GSK-3β level.It can enhance insulin signal transduction and promote liver glycogen synthesis，so as to play a hypoglycemic effect.The combination of metformin and Didang Qigui decoction can enhance its hypoglycemic effect in the treatment of T2DM.
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Memo
基金项目：陕西省教育厅科研项目（19JK0227）；伤寒学与经方辨治疑难病一流创新团队（2019-QN04）；陕西省伤寒学与经方治疗辨治疑难病研究室资助项目