[1] LI Qiang PANG Zhonghui TAO Feifei XU Wenlong,.Functional Research of Total Saponins of Dioscorea Bicolor on Improving Osteoarthritis in Guinea Pigs by Inhibiting Degradation of Cartilage Matrix [J]. Modern Traditional Chinese Medicine, 2022, 1(06): 37-41. [doi: 10.13424/j.cnki.mtcm.2022.06.008]
Functional Research of Total Saponins of Dioscorea Bicolor
on Improving Osteoarthritis in Guinea Pigs by
Inhibiting Degradation of Cartilage Matrix
- Article number:
- 1672-0571(2022)06-0037-05
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the therapeutic effect of total saponins of Dioscorea bicolor (TRI) on guinea pigs with osteoarthritis (OA) and its possible mechanism. Methods 40 guinea pigs were divided into control group (Con), osteoarticular modeling group (OA), total saponins of Dioscorea bifida (TRI) group, and total saponins of Achyranthes bidentata (TSA) group. HE staining was used to observe the cartilage morphology of guinea pigs in each group; Safranine O'fixation green staining was used to observe the cartilage matrix of guinea pigs in each group; The expression of type Ⅱ collagen was detected by immunofluorescence; Enzyme linked immunosorbent assay (ELISA) was used to detect interleukin-1 (IL-1), tumor necrosis factor α （TNF-α）and matrix metalloproteinase 13 (MMP-13) in bone joint fluid of guinea pigs in each group; The expression of Neil like molecule-1 (Nell-1) in bone joints of guinea pigs in each group was observed by immunohistochemistry. Results Both TRI and TSA significantly alleviated the osteoarthritis injury and cartilage matrix degradation of guinea pigs in OA group, significantly promoted the expression of type II collagen and Nell-1, and decreased the inflammatory factors IL-1, TNF-αand MMP-13. The therapeutic effect of TRI group was better than that of TSA group.Conclusion The total saponins of Dioscorea septicerae may play a therapeutic role in osteoarthritis by promoting the expression of Nell-1.
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Memo
基金项目：陕西省科技厅科技攻关项目（2018K16-09-07）