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[1] LAI Jing NI Lin LIN Ruili JIN Wanjun LIU FuqiangSONG Pingshun MA Xiao TENG Baoxia,.Identification of Mixed and Counterfeit Zang Chaihu fromBei Chaihu Based on ITS Sequence PCR-RFLP [J]. Modern Traditional Chinese Medicine, 2023, (05): 105-112. [doi: 10.13424/j.cnki.mtcm.2023.05.020]

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Identification of Mixed and Counterfeit Zang Chaihu fromBei Chaihu Based on ITS Sequence PCR-RFLP

Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN] Volumes & Issues: 2023 05 pagination: 105-112 Column: Publication date: 2023-09-20

Article number:: 1672-0571(2023)05-0105-08

Author(s):: LAI Jing NI Lin LIN Ruili JIN Wanjun LIU FuqiangSONG Pingshun MA Xiao TENG Baoxia; Gansu Provincial Institute for Drug Control/National Key Laboratory for Quality Control ofTraditional Chinese Medicine and Dried Pieces，Lanzhou 730013,China

Keywords:: Key words:Zang Chaihu; Bei Chaihu; ITS; PCRRFLP; Molecular identification

ZTFLH:: R282.5

DOI:: 10.13424/j.cnki.mtcm.2023.05.020

Code:: A

Abstract:: Abstract：Objective To identify the adulteration of Bupleurum chinense with Zang Bupleurum chinense based on ITS sequence using PCRRFLP method.Methods Analysis and screening were conducted to identify the specific restriction endonuclease Ase Ⅰ in Tibetan Chaihu.Primer Premier 5.0 was used to design specific primers，optimize the PCR amplification conditions and enzyme digestion test，and evaluate the accuracy of this method.Results The target fragment amplified by PCR was 331 bp，and the established PCR reaction method was adaptable to different enzymes.Restrictive endonuclease Ase Ⅰ cleaves Zang Bupleurum into 79 bp and 252 bp fragments，while Northern Bupleurum and other Bupleurum cannot be cleaved，and the detection limit for adulteration is 1%.Conclusion The PCR-RFLP method has achieved accurate identification of the counterfeit Zang Chaihu in Beichaihu.

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Memo

基金项目：国家药监局中药材及饮片质量控制重点实验室项目(2021GSMPA-KL03)

Last Update: 2023-09-22