[1] Sun Lijun Sun Yuru Jiang Mei Li Cuijuan Zhang Qi Sun Yaoguang,.Effect of Chinese Herbal Medicine for Invigorating Kidney on Expression of ERK1 in Hippocampus of Rats with Kidney Deficiency [J]. Modern Traditional Chinese Medicine, 2018, (06): 144-148. [doi: 10.13424/j.cnki.mtcm.2018.06.047]
Effect of Chinese Herbal Medicine for Invigorating Kidney on
Expression of ERK1 in Hippocampus of Rats with Kidney Deficiency
- Article number:
- 1672－0571(2018)06－0144－05
- Keywords:
- kidney － invigorating herbs; kidney storing will; constitution of kidney deficiency; learning and memo- ry; ERKI
- ZTFLH:
- R285. 5
- Code:
- A
- Abstract:
- To observe the effect of kidney － invigorating herbs on learning and memory a- bility and expression of ERK1 protein in hippocampus of rats with kidney － deficiency syndrome，and to explore the intrinsic molecular mechanism of improving learning and memory ability of kidney － deficien- cy syndrome from the perspective of synaptic plasticity． Method: The rat model of congenital insufficiency and acquired intimidation of kidney deficiency was established by "cat scare rat" model． The rats were divided into four groups: model group，kidney － invigorating group，heart － tonifying group and liver － soothing group． The kidney － invigorating group，heart － tonifying group and liver － soothing group were given Liuwei Dihuang Pills，Tianwang Buxin Bolus and Xiaoyao pills respectively． The blank group wasproduced from normal pregnant rats． Morris water maze test was used to test the learning and memory a- bilities of rats in each group． The body weight and brain weight were used to compare the growth and development． The expression of ERK1 positive cells in hippocampus was detected by immunohistochem- istry． Result: Compared with the blank group，the total distance of the model group increased significant- ly． The target area retention time，body weight，brain weight and ERK1 positive expression in the hip- pocampus were significantly reduced within 30s． Compared with the model group，the total distance of the kidney － invigorating group，heart － tonifying group and liver － soothing group were all significantly decreased，the target area retention time，body weight，brain weight and hippocampus ERK1 positive expression increased significantly within 30s; Compared with the heart － tonifying group and liver － soothing group，the total distance of kidney － invigorating group decreased significantly，and the reten- tion time，body weight，brain weight and positive expression of ERK1 in hippocampus increased signifi- cantly in 30 seconds． Conclusion: Kidney － invigorating herbs can improve the learning and memory of rats with kidney deficiency by increasing the expression of learning memory protein ERK1 in hippocam- pus; the expression of ERK1 in hippocampus is related to the theory of“kidney storing will”．
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Memo
收稿日期:2018 －09 －10 编辑:文颖娟*
基金项目:陕西省科技厅社发项目(2018SF －312)