[1] LI Tao TAO Shan LI Qian GUO DongyanFAN Yu,.Protective Effect and Mechanism of Qizhu Capsule on CCl4 induced Chronic Liver Injury in Rats [J]. Modern Traditional Chinese Medicine, 2021, (06): 16-21. [doi: 10.13424/j.cnki.mtcm.2021.06.004]
Protective Effect and Mechanism of Qizhu Capsule on CCl4
induced Chronic Liver Injury in Rats
- Article number:
- 1672-0571(2021)06-0016-06
- Keywords:
- Key words:Qizhu capsule; Carbon tetrachloride; Chronic liver injury; Oxidative stress injury; Inflammatory response; TNF-α/NF- κB pathway
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract：Objective To observe the protective effect of Qizhu Capsule on chronic liver injury induced by carbon tetrachloride (CCl4) in rats and explore its mechanism.Methods SD rats were randomly divided into blank control group，model group，positive control group and high，medium and low dose treatment groups of Qizhu capsule.Except for the blank control group，rats in other groups were intraperitoneally injected with 40% CCl4 olive oil solution，twice/Week for 8 weeks.A rat model of CCl4 induced chronic liver injury was established.From the first day after successful modeling，the drug was continuously administered by gavage until the end of the 8th week.The liver organ index was calculated，the contents of ALT and AST in serum were detected by microplate method，and the pathological changes of liver were observed by HE staining；The activities of SOD,GSH-Px and the content of MDA in liver tissue were detected by biochemical method；TNF-α,NF-κB expression and content changes in liver tissue was detected by Western blot.Results Compared with the model control group，each dose of Qizhu capsule group could significantly improve the pathological changes of liver tissue，reduce fiber deposition，reduce the activities of ALT and AST in serum，significantly increase the activities of SOD and GSH-Px，reduce the content of MDA and significantly inhibit TNF-α and NF-κB expression in liver tissue (P＜0.05).Conclusion Qizhu capsule has obvious protective effect on CCl4 induced chronic liver injury in rats，and its mechanism may be related to reducing oxidative stress injury and regulating TNF-α/ NF- κB pathway inhibition is related to the symptomatic response. Abstract：Objective To observe the protective effect of Qizhu Capsule on chronic liver injury induced by carbon tetrachloride (CCl4) in rats and explore its mechanism.Methods SD rats were randomly divided into blank control group，model group，positive control group and high，medium and low dose treatment groups of Qizhu capsule.Except for the blank control group，rats in other groups were intraperitoneally injected with 40% CCl4 olive oil solution，twice/Week for 8 weeks.A rat model of CCl4 induced chronic liver injury was established.From the first day after successful modeling，the drug was continuously administered by gavage until the end of the 8th week.The liver organ index was calculated，the contents of ALT and AST in serum were detected by microplate method，and the pathological changes of liver were observed by HE staining；The activities of SOD,GSH-Px and the content of MDA in liver tissue were detected by biochemical method；TNF-α,NF-κB expression and content changes in liver tissue was detected by Western blot.Results Compared with the model control group，each dose of Qizhu capsule group could significantly improve the pathological changes of liver tissue，reduce fiber deposition，reduce the activities of ALT and AST in serum，significantly increase the activities of SOD and GSH-Px，reduce the content of MDA and significantly inhibit TNF-α and NF-κB expression in liver tissue (P＜0.05).Conclusion Qizhu capsule has obvious protective effect on CCl4 induced chronic liver injury in rats，and its mechanism may be related to reducing oxidative stress injury and regulating TNF-α/ NF- κB pathway inhibition is related to the symptomatic response.
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Memo
基金项目：国家自然科学基金青年项目（81703925）；陕西省科技厅社发研究计划项目（2016SF-367）；陕西中医药大学创新团队项目（2019-YL14）；陕西省中药基础与新药研究重点实验室开放基金项目（2017KF03）