Pre Next

PDF Download

[1] Yue Ming Ma Li Quan Min Zhang Enhu,.Determination of Ginsenoside Rg1 in Huaxian Gongxue Capsules by HPLC [J]. Modern Traditional Chinese Medicine, 2019, (03): 95-98,119. [doi: 10.13424/j.cnki.mtcm.2019.03.030]

Copy

Determination of Ginsenoside Rg1 in Huaxian Gongxue Capsules by HPLC

Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN] Volumes & Issues: 2019 03 pagination: 95-98,119 Column: Publication date: 2019-05-30

Article number:: 1672-0571(2019)03-0095-05

Author(s):: Yue Ming 1 Ma Li 1 Quan Min 1 Zhang Enhu 2; 1. Xianyang Central Hospital, Xianyang China, 712000; 2. Shaanxi University of Chinese Medicine, Xianyang China, 712046

Keywords:: Huaxian Gongxue Capsules; ginsenoside Rg1; High Performance Liquid Chromatography( HPLC)

ZTFLH:: R284

DOI:: 10.13424/j.cnki.mtcm.2019.03.030

Code:: A

Abstract:: To determine the content of ginsenoside Rg1 in Huaxian Gongxue Capsules and establish the quality control standard for Huaxian Gongxue Capsules. Methods: high performance liquid chromatography ( HPLC) was adopted, chromatographic column: Kromosil ODS C18 ( 5 micron, 200 x 4. 6 mm) ; mobile phase: acetonitrile -0. 05% phosphoric acid solution ( 24: 76) ; detection wavelength: 203 nm; flow rate: 1. 0 ml/ min. Results: There was a good linear relationship for ginsenoside Rg1 ranging 0. 85 - 8. 50 UG ( r = 0. 9994, n = 6) , and the average recovery was 105. 80% ( RSD = 0. 74%, n = 6) . Conclusion: Determination of ginsenoside Rg1 in Huaxian Gongxue Capsules by HPLC is accurate, simple, stable and reliable. It can be used for the content control of Huaxian Gongxue Capsules.

References:

[1]李明,李玺,权乾坤,等.老年痴呆模型中人参皂苷Rg1预处理对大鼠脑片中突触素表达的影响[J].西部医学,2018,30(4):485-488.
[2]李明,李玺,权乾坤,等.人参皂苷Rg1对冈田酸诱导的AD样脑片模型的影响[J].西部医学,2016,28(6):763-767.
[3]胡楚璇,刘洁,郭小东.人参皂苷Rg1镇痛抗炎实验研究[J].中药材,2013,36(3):464-467.页)
[4] Jia L, Wang W Y, Zhou L M, et al. Antimotion sicknesseffects of ginsenosides combined with dexamethasone inrats[ J] . Zhong xi yi jie he xue bao = Journal of Chineseintegrative medicine, 2010, 8( 4) : 358.
[5] Radad K, Gille G, Moldzio R, et al. Ginsenosides Rb _{1 and Rg 1 effects onmesencephalic dopaminergic cells stressed with glutamate [J] . Brain research, 2004, 1021( 1) : 41 -53.

[6] Lee Y J, Chung E, Youl Lee K, et al. Ginsenoside -Rg1, one of the major active molecules from Panaxginseng , is a functional ligand of glucocorticoid receptor[ J] . Molecular and cellular endocrinology, 1997,133( 2) : 135 -140.

[7] Chan R Y K, Chen W F, Dong A, Guo D, et al. Estrogen - like activity of ginsenoside Rg1 derived Panax notoginseng[ J] . Journal of Clinical Endocrinology & Metabolism, 2002, 87( 8) : 3691 -3695,424.

[8]王培培,王雪丹,张勇,等.植物性雌激素对子宫肌瘤模型大鼠血清中E2?P及EGF的影响[J].卫生职业教育,2013,31(22):81-82.

[9]李海,陈建海,王金花,等.山茶籽对去卵巢大鼠子宫和相关激素水平的影响[J].中国老年学杂志,2013,33(19):4783-4785.

[10]吉梅,陈超,林流芳,等.植物性雌激素对子宫内膜及异位子宫内膜的影响[J].生殖与避孕,2012,32(3):185-189.

[11] Leung K W, Wong A S. Pharmacology of ginsenosides: aliterature review[ J] . Chin Med, 2010, doi: 10. 1186 /1749 -8546 -5 -20,

[12] LIU H F, YANG J L, DU F F, et al. Absorption and disposition of ginsenosides after oral administration of Panaxnotoginseng extract to rats[ J. Drug MetabDispos, 2009, 37( 12) :2290 -2298.

[13]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:481-482.}

Last Update: 2019-05-15