[1] Zhan Yongjiu Meng Yifeng Chen Jia Yan Xuemei Xu Feng Zhu Xiaojing,.Effect of Modified Liuwei Dihuang Decoction on the Proliferation of Human Dermal Papilla Cells and the Expression of Vascular Endothelial Growth Factor [J]. Modern Traditional Chinese Medicine, 2019, (05): 98-102. [doi: 10.13424/j.cnki.mtcm.2019.05.030]
Effect of Modified Liuwei Dihuang Decoction on the Proliferation of Human Dermal Papilla Cells and the Expression of Vascular Endothelial Growth Factor
- Article number:
- 1672-0571(2019)05-0098-05
- ZTFLH:
- R285.6
- Code:
- A
- Abstract:
- To observe the effect of Modified Liuwei Dihuang Decoction on the proliferation and secretion of vascular endothelial growth factor ( VEGF) in human dermal papilla cells. Methods: Human dermal papilla cells cultured in vitro were treated with rat serum containing Modified Liuwei Dihuang Decoction at low, medium and high concentrations. Cell proliferation activity was measured by MTT method, and the content of VEGF protein in cell culture supernatant was detected by ELISA method. Results: Compared with the control group, the effect of Modified Liuwei Dihuang Decoction on cell proliferation was not significant ( P > 0. 05) ; in the secretion of vascular endothelial growth factor, the medium and high concentration of Modified Liuwei Dihuang Decoction group had obvious secretion promoting effect, and had dose - effect relationship, the difference was statistically significant ( P < 0. 05, P < 0. 01) . Conclusion: Modified Liuwei Dihuang Decoction has no significant effect on the proliferation of human dermal papilla cells, but it can significantly promote the secretion of VEGF in human dermal papilla cells. Modified Liuwei Dihuang Decoction may promote hair growth by increasing the secretion of VEGF in dermal papilla cells.
References:
[1]王纪云.六味地黄丸治愈脱发1例[J].山西中医,1999,15(1):24.
[2]张理保.六味地黄丸治疗斑秃22例[J].湖南中医杂志,2001,17(4):44.
[3]曹玉杰.中西医结合治疗斑秃疗效观察[J].中国民间疗法,2014,22(10):64.
[4]占永久,詹业顺,张晓杰.“止血”治脱发———“发为血之余”之理论发挥[J].中华中医药杂志,2014,29(5):1524-1527.
[5]何天林.重用补骨脂治疗脱发1例[J].中国社区医师,2007,9(166):58.
[6]占永久.旱莲草“乌须发”作用浅析[J].中医药导报,2015,8:84-85.
[7] Xiong Y, Liu Y, Song Z, et al. ldentification of Wnt/ beta- catenin signaling pathway in dermal papilla cells of human scalp hair follicles: TCF4 regulates the proliferation and secretory activity of dermal papilla cell [ J] . J Dermatol,2014,41 ( 1) :84 -91.
[8]林恩,祝宁侠,蔡博治,等.LncRNAs和miRNAs在毛乳头细胞衰老与毛囊再生中的作用[J].中国美容整形外科杂志,2018,29(7):435-437.
[9]王磊,范卫新.毛囊生物学基础知识[J]中国医学文摘?皮肤科学,2016,33(4):409-414.
[10]李维珊,柯昌能,齐德丽.EGF?bFGF对促进人毛乳头细胞生长及分化的影响[J].临床医学工程,2014.2(21):139-140.
[11]朱海琴,范卫新,张卉.甘草提取物对人毛乳头细胞增殖和分泌血管内皮生长因子的影响[J].临床皮肤科杂志,2007,36(4):201-203.
[12] Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF mediated angiogenesis[ J] . J Clin Invest,2001,107( 4) :409 -417.
[13]范卫新,朱文元.55种中药对小鼠触须毛囊体外培养生物学特性的研究[J]临床皮肤科杂志,2001,30(2):81-84.
[14]张兴洪,范卫新.何首乌?女贞子等中药煎剂对体外培养的猪毛囊毛发生长的影响[J].中华皮肤科杂志,2005,38(2):102-104.
[15]孙宇,杨淑霞,涂平,等.女贞子等对人头皮毛囊体外培养影响的研究[J].中华皮肤科杂志,2005.38(5):303-305.
[16]吴巧云.红花?当归和生侧柏叶煎剂对鼠毛囊生长影响的体外研究[D].杭州:浙江大学,2006.
[17]李东海,陈罗娣,齐庆,等.补发1号方促毛乳头细胞增殖及其分子机制的实验研究[J].中国现代医学杂志,2012,22(31):25-29.
[18]何红梅,朱红霞,刘强,等.何首乌提取物对C57BL/6J小鼠毛囊生长和毛发生长周期的影响[J].中国实验方剂学杂志,2012,18(23):216-219.
[19]徐莲,吴岚,王雪,等.黄芪?何首乌?女贞子?菟丝子混合提取物对体外培养毛囊生长的影响及药理作用研究[J].现代生物医学进展,2014,22(8):4201-4204.
[20]王任,袁婷,周雯,等.桑柏生发方对毛发再生过程中VEGF等细胞因子的影响[J].中医药导报,2016,22(15):24-27.
[21]万力生,范红霞.中医治疗脱发的方药统计与述评[J].浙江中西医结合杂志,2000,10(9):564-565.
[22]沈璐,陈科力.中医药治疗脱发的研究与分析[J].中南民族大学学报(自然科学版),2011,30(1):42-45.
[23]何天林.重用补骨脂治疗脱发1例[J].中国社区医师,2007,9(166):58.
Memo
基金项目：苏州市科技发展计划项目（ＳＹＳＤ２０１５１５６）