[1] Li Cuijuan, Yang Jian, Gong Zhendong, et al. A Study on the Change Rule of Parotid Gland AQP1 in Rats with Normal Constitution - Kidney Deficiency Constitution and Kidney Deficiency Syndrome [J]. Modern Traditional Chinese Medicine, 2020, (06): 9-14. [doi: 10.13424/j.cnki.mtcm.2020.06.003]
A Study on the Change Rule of Parotid Gland AQP1 in Rats with Normal Constitution - Kidney Deficiency Constitution and Kidney Deficiency Syndrome
- Article number:
- 1672-0571(2020)06-0009-06
- Keywords:
- kidney deficiency constitution; kidney deficiency syndrome; saliva flow; saliva osmotic pressure; AQP1
- ZTFLH:
- R2-03
- Code:
- A
- Abstract:
- Objective To investigate the change rules of parotid saliva flow, osmotic pressure, and AQP1 during the dynamic evolution among rats with normal constitution, kidney deficiency constitution and kidney deficiency syndrome. Methods According to the TCM theory of “ kidney injury fear ” , an animal model of kidney deficiency in the offspring rats was made by means of cats - scaring - pregnant mice. On this basis, an animal model of kidney deficiency syndrome was made by intramuscular injection of hydrocortisone injection. The normal control group, kidney deficiency constitution and kidney deficiency syndrome were observed in terms of their changes of salivary flow, osmotic pressure and parotid gland AQP1. Results ①General comparison: During the modeling period, the rats in the normal control group gained significant weight, good mental state, thick and shiny fur, normal diet, and good bowel movements. Compared with the normal control group, the rats in the kidney -deficiency physique group were slightly lighter, weakened, with hair gloss. Some rats had hair loss, poor food intake, soft stools and excessive urination. The rats in the kidney -deficiency syndrome group have the lightest average weight, sparse hair, all have hair loss, and they are timid and easy to be shocked. They like to hug together to keep warm with low spirt less activity, less food intake, more irregular stools, and more urination. ②Saliva flow test: Compared with the normal control group, the saliva secretion and flow of the kidney deficiency constitution group were decreased, which was statistically significant ( P <0.05) , and the saliva flow of the kidney deficiency syndrome group was significantly reduced, which was statistically significant ( P <0.01) . ) ; ③ Saliva osmotic pressure test: Compared with the normal control group, the saliva osmotic pressure of the kidney - deficiency constitution group and the kidney – deficiency syndrome group were lower with significant statistical difference ( P <0. 01) ; ④Parotid gland tissue AQP1 immunohistochemical test: Compared with the normal control group, the expression of AQP1 in the parotid gland of the kidney -deficiency constitution group and the kidney - deficiency syndrome group was reduced, which was statistically significant ( P <0.01) ; Compared with the kidney -deficiency constitution group, the expression of AQP1 in the kidney -deficiency syndrome group was reduced, but there was no statistical difference ( P >0. 05) ; ⑤ Western blot detection of parotid gland tissue AQP1: Compared with the normal control group, the expression of AQP1 in the parotid gland of the kidney deficiency constitution group and the kidney deficiency syndrome group decreased, which was statistically significant ( P <0. 01) ; Compared with the kidney deficiency constitution group, the expression of AQP1 in the kidney deficiency syndrome group decreased, with statistical difference ( P <0.05) . Conclusion Along with the dynamic evolution process of “ normal constitution, kidney deficiency constitution and kidney deficiency syndrome” , rats＇saliva flow, osmotic pressure and parotid gland AQP1 expression have all changed, and with the aggravation of kidney deficiency, the changes in corresponding indicators are more obvious. It shows that the secretion of saliva is indeed closely related to the ups and downs of renal function, and its internal mechanism may be related to the expression of parotid gland AQP1.
References:
[1]于健宁.陶筱娟辨治干燥综合征经验[J].中医杂志,2012,53(12):1062-1063.
[2]刘娜,李翠娟,巩振东,等.从肾论治干燥综合征探析[J].吉林中医药,2019,39(8):984-986,1007.
[3]王鹏飞,姜萍,刘英.从肾论治干燥综合征[J].南京中医药大学学报,2019,35(4):384-386.
[4]魏方,安颂歌,陈琳,等.玉液汤治疗老年性口腔干燥症经验体会[J].中国中医药现代远程教育,2019,17(16):60-62.
[5]马武开,贾二涛,王莹,等.干燥综合征模型小鼠涎腺AQP1?AQP5表达的研究[J].山东医药,2011,51(24):46-48.
[6]杨军,肖林,孙远,等.人涎腺水通道蛋白的定位[J].第三军医大学学报,2002,24(8):967-969.
[7]李翠娟,孙理军,巩振东.代谢组学与“正常体质-病理体质-证候”动态演变关系的研究思路探讨[J].湖北中医杂志,2011,33(10):46-47.
[8]李翠娟,刘子蠧.“正常体质-肾虚体质-肾虚证候”大鼠腮腺病理组织学及唾液BUN?UA?CREA变化研究[J].现代中医药,2015,35(6):108-110,122.
[9]李翠娟,刘子蠧,孙理军,等.“正常体质-肾虚体质-肾虚证候”唾液生化指标变化研究[J].中国中医药信息杂志,2016,23(6):47-50.
[10]巩振东,李翠娟,刘子蠧,等.“正常体质-肾虚体质-肾虚证候”唾液代谢组学研究[J].中华中医药杂志,2017,32(11):5084-5087.
[11]李碧霞.乌梅喷雾剂对Wistar大鼠颌下腺放射性损伤后功能影响的研究[D].广州:广州中医药大学,2017.
[12]肖林,杨军,胡静,等.水通道蛋白-1,5在干燥综合征小鼠颌下腺中的表达[J].中国口腔颌面外科杂志,2005,3(4):325-329.
Memo
基金项目:国家自然科学基金项目(81202614);陕西省教育厅重点实验室科研计划项目(17JS032,18JS030);陕西省中医药管理局科研项目(2019-ZZ-JC016),第四批全国中医基础/临床优秀人才研修项目(国中医药人教发[2017]24号);陕西省中医药管理局中医藏象理论重点研究室建设项目(SZDYJS-2);咸阳市中医体质与疾病防治重点实验室重点研发计划项目(2019K01-53);陕西中医药大学中医经典理论研究创新团队建设项目(2019-YL01);2020年陕西中医药大学校级科研课题研究生创新项目(2020CX08)