[1] Wang Xiaojuan, Li Juan, Zheng Fanghao,.A Study on Adverse Dermatic Reactions of Huoxue Tongluo Liniment on New Zealand Rabbit Skin [J]. Modern Traditional Chinese Medicine, 2020, (06): 15-19. [doi: 10.13424/j.cnki.mtcm.2020.06.004]
A Study on Adverse Dermatic Reactions of Huoxue Tongluo Liniment on New Zealand Rabbit Skin
Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN]
Volumes & Issues:
2020 06
pagination:
15-19
Column:
Publication date:
2023-11-20
- Article number:
- 1672-0571(2020)06-0015-05
- Keywords:
- Huoxue Tongluo Liniment; Acute Toxicity; New Zealand Rabbit; Skin Administration; Skin Adverse Reaction
- ZTFLH:
- R289.6
- Code:
- A
- Abstract:
- Objective To observe the irritation and other adverse reactions caused by external application of Huoxuetongluo Liniment to intact or damaged skin of New Zealand rabbits, thereby providing a basis for clinical medication and toxicological studies for further application of hospital preparations. Methods 40 New Zealand rabbits qualified for quarantine were selected and divided into 4 groups: intact skin negative group, intact skin administration group, broken skin negative group, broken skin administration group with 10 rabbits in each, half male and half male. Both sides of the intact skin or broken skin of each group were administered at the same time, 96cm2/ side ( 8cm ×12cm/ side) , 8mL/ side/head/ time, the administration concentration was 2. 25g crude drug/ mL. Negative control group was given the same volume and range of pure water as control factors, administered 3 times a day. Then experimenters observe and record the erythema and edema at the administration site and score them, check the hematology, blood biochemistry and coagulation function indexes, and visually observethe changes of various organs and tissues after gross autopsy. Results There was no obvious irritation in the skin of the intact skin and broken skin negative control group animals; there was no obvious abnormality in general observation, and the weight showed an upward trend. There were no obvious abnormal changes in blood biochemistry and hematology. No obvious abnormal changes were seen. Both intact skin and damaged skin administration groups can produce transient and recoverable irritation. Animal behavior, glandular secretion, respiratory and fecal characteristics, weight, hematology, blood biochemistry, general anatomy, etc. were not obviously abnormal, and the weight is increasing. Conclusion There are no obvious toxic reactions and toxic target organs when the intact skin is administered with Huoxuetongluo liniment while the damaged skin has moderate skin irritation, but no obvious toxic target organs are found.
References:
[1]董辉,李先娜,孙晖.川乌毒性研究概况[J].黑龙江科技信息,2015,19(31):102.
[2]孙凤姣,张译丹,吴锦,等.生川乌醇提物致心?脑毒性的表现及其对血管内皮的影响[J].中草药,2017,48(6):1178-1182.
[3]苗鑫,张晓菲,刘丹丹,等.草乌毒性及相关模型构建的研究进展[J].中药新药与临床药理,2018,29(6):836-839.
[4]张德放,邱维彬,王坤.马钱子的毒性及中毒解救的研究[J].辽宁中医杂志,2011,38(4):713-714.
[5]林锦霞.抗炎镇痛中药马钱子外用制剂的研究进展[J].医学综述,2018,24(16):3259-3263.
[6]中华人民共和国国家药典委员会.中华人民共和国药典2015年版(一部)[M].北京:中国医药科技出版社,2015:39,50,236.
Memo
基金项目:广东省中医药局中医药强省建设专项资金-医院中药制剂开发项目(粤财社[2015]77号)
通讯作者:王晓娟,主管中药师?E-mail:wangxjjd1986@126.com
Last Update:
2020-11-26