[1] HAO Yelu NIU Wenmin,.Effect of Early Intervention of Volatile Oil from Acorus Tatarinowii on Behavior of PTSD Model Rats [J]. Modern Traditional Chinese Medicine, 2022, 1(01): 28-34. [doi: 10.13424/j.cnki.mtcm.2022.01.005]
Effect of Early Intervention of Volatile Oil from Acorus
Tatarinowii on Behavior of PTSD Model Rats
- Article number:
- 1672-0571(2022)01-0028-07
- Keywords:
- Key words：Post traumatic stress disorder; Early intervention; Volatile oil of Acorus tatarinowii; Nasal sniffing; Olfactory nerve conduction pathway; Hippocampus; Gucocorticoid receptor
- ZTFLH:
- R285
- Code:
- A
- Abstract:
- Abstract：Objective To observe the effect of early intervention of volatile oil from Acorus tatarinowii on behavior of PTSD model rats, and to explore the related mechanism of hippocampal GR gene. Methods PTSD rat model was established by single prolonged stress. The rats were randomly divided into control group, model group, olfactory nerve transection + model group, model + Acorus tatarinowii olfactory group, olfactory nerve transection + model + Acorus tatarinowii olfactory group. RTC was used to detect the irritability of rats, the anxiety level of rats was detected by opening experiment and elevated cross maze experiment,and the expression level of GR protein in rat hippocampus was measured by Western blot. Results The percentage of central activity distance of PTSD model rats in opening experiment was significantly increased after the early intervention of volatile oil of Acorus tatarinowii (P<0.05), the percentage of open arm entry times in elevated cross maze test increased significantly (P＜0.05), and the score in rejection response test decreased (P>0.05), indicating that the early intervention of volatile oil of Acorus tatarinowii reduces the irritability and anxiety level of PTSD model rats, and its effect depends on the integrity of olfactory pathway structure.In addition, through the early intervention of volatile oil of Acorus tatarinowii, the expression of GR protein in hippocampus of PTSD model rats is down-regulated. Conclusion The olfactory pathway of volatile oil of Acorus tatarinowii plays an important role in the early intervention of PTSD. The mechanism may be related to hippocampal GR gene.
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Memo
基金项目：中国人民解放军联勤保障部队第九四〇医院院内青年培育项目（2021yxky067）