[1] LI liang CHEN Yucheng WANG Qiang ZHOU Jie,.Effect of Mahuang Fuzi Xixin Decoction on PI3K/Akt/mTOR Signal Pathway in Yang Deficiency Non-small Cell Lung Cancer Mice [J]. Modern Traditional Chinese Medicine, 2022, 1(02): 43-48. [doi: 10.13424/j.cnki.mtcm.2022.02.008]
Effect of Mahuang Fuzi Xixin Decoction on PI3K/Akt/mTOR Signal Pathway in Yang Deficiency Non-small Cell Lung Cancer Mice
- Article number:
- 1672-0571(2022)02-0043-06
- ZTFLH:
- R256.19
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the effect of Mahuang Fuzi Xixin decoction on PI3K/Akt/mTOR signal pathway in Yang deficiency non-small cell lung cancer mice.Methods C57BL/6 mice were randomly divided into model group，chemotherapy group，Mahuang Fuzi Xixin Decoction high，medium and low dose group (traditional Chinese medicine high，medium and low group)，combined group (Mahuang Fuzi Xixin Decoction high dose group+cisplatin).After the successful establishment of the model，the drugs were administered in groups，and the expression level of PI3K/Akt/mTOR pathway related proteins in mouse tumor tissues was detected by Western blot.Results 1.Mahuang Fuzi Xixin decoction and chemotherapeutic drugs can reduce the protein expression levels of PI3K p85a and PI3K p110a.It can be considered that there is interaction and synergy between the two drugs for the time being；2.Mahuang Fuzi Xixin decoction combined with chemotherapy drugs has obvious synergistic effect on reducing the expression of Akt and p-Akt protein；3.The combination of Mahuang Fuzi Xixin decoction and cisplatin has a synergistic effect on weakening the expression of mTOR and p-mTOR proteins.Conclusion This experiment discusses the mechanism of Mahuang Fuzi Xixin decoction blocking PI3K/Akt/mTOR signal pathway in Yang deficiency non-small cell lung cancer mice from the aspect of overall efficacy.It is found that Mahuang Fuzi Xixin decoction can reduce the expression level of PI3K，Akt and mTOR protein to a certain extent，and has a certain inhibitory effect on the transmission of PI3K/Akt/mTOR signal pathway.
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Memo
基金项目：天津市教委科研计划项目（2019KJ069）