[1] DUO Jiezhuoma HUANG Yanfeng MEI Zhinan ZHOU Shengjun PENG Yi CHEN Weiwu,.Research of Twenty Five Flavor Pearl Tablet Function and Mechanism on Anti PM2.5 [J]. Modern Traditional Chinese Medicine, 2022, 1(03): 43-48. [doi: 10.13424/j.cnki.mtcm.2022.03.008]
Research of Twenty Five Flavor Pearl Tablet Function and Mechanism on Anti PM2.5
- Article number:
- 1672-0571(2022)03-0043-06
- Keywords:
- Key words：Twenty five flavor pearl tablets; Nano activated carbon particles; Zebrafish; Macrophages; Intestinal peristalsis
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract：Objective To observe whether twenty five flavor pearl tablets have the effect of anti fine particle nano activated carbon (PM2.5 analog) and explore its potential mechanism.Methods Zebrafish were treated with different concentrations of 25 flavor pearl tablets (62.5，125，250，500 and 1000 μg·mL-1） to determine the maximum tolerance concentration of zebrafish to 25 flavor pearl tablets.Further，intravenous injection (iv) and intramuscular injection (im) of nano activated carbon were used to simulate the functional evaluation model of macrophages phagocytizing nano activated carbon particles and nano activated carbon particles secreted into the intestine in zebrafish.Twenty five flavor pearl tablets were given to intervene，and the number of macrophages phagocytizing nano activated carbon，phagocytosis promotion rate and the number of nano activated carbon secreted into the intestine in zebrafish were counted.The evaluation model of intestinal peristalsis of zebrafish was established by giving Nile Red aqueous solution to explore the effect of 25 flavor pearl tablets on intestinal peristalsis function of zebrafish.Finally，transgenic zebrafish with fluorescent neutrophils were injected with lipopolysaccharide (LPS) into the yolk sac to establish the zebrafish inflammation model.The number of neutrophils in the yolk sac of zebrafish in the control group and the treatment group were observed and counted.Results The maximum tolerance concentration of zebrafish to twenty five flavor pearl tablets was 250 μg·mL-1；Compared with the control group，the number of macrophages phagocytizing nano activated carbon fine particles increased and the phagocytosis rate of macrophages increased in the treatment group.In the administration group，the secretion of nano activated carbon particles in zebrafish intestine did not change，but the promotion rate of intestinal peristalsis increased significantly.There was no significant improvement in neutrophil infiltration in zebrafish yolk sac in the administration group.Conclusion Twenty five flavor pearl tablet has anti PM2 function.The effect of activated carbon fine particles may be related to promoting the phagocytosis of fine particles by macrophages and the clearance of intestinal fine particles.
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Memo
基金项目：国家新药创制科技重大专项（2017ZX09031059）