[1] CHAI MengyinKOU Buxin DOU Shuangshuang LIU Xiaoni,.Anti Acute Liver Injury and Anti Fatigue Effects of Huangqi Ultrafine Powder and Changes of Astragaloside IV Content [J]. Modern Traditional Chinese Medicine, 2022, 1(05): 26-32. [doi: 10.13424/j.cnki.mtcm.2022.05.005]
Anti Acute Liver Injury and Anti Fatigue Effects of Huangqi
Ultrafine Powder and Changes of Astragaloside IV Content
- Article number:
- 1672-0571(2022)05-0026-07
- Keywords:
- Key words:Astragalus; Acute liver injury; Swimming experiment; Superfine powder; Astragaloside IV
- ZTFLH:
- R283
- Code:
- A
- Abstract:
- Abstract：Objective To observe the anti acute liver injury and anti fatigue effects of Huangqi ultrafine powder and the changes of astragaloside IV content，so as to provide scientific basis for the application of Huangqi ultrafine powder.Methods Astragalus pieces were crushed into ultra-fine powder by low-temperature ultra-fine pulverization technology.The microscopic characterization and particle size characterization were observed by microscope and laser particle sizer.The content of Astragaloside IV in Astragalus pieces and Huangqi ultra-fine powder was detected by HPLC.Kunming mice were randomly divided into control group (Con)，model group (M)，Huangqi decoction group (HQY，10 g·kg-1),Huangqi ultra-fine powder high-dose group (HQFH，10 g·kg-1)，Huangqi ultra-fine powder medium dose group (HQFM，2 g·kg-1)，Huangqi ultra-fine powder low-dose group (HQFL，0.4 g·kg-1).Except for the control group and the model group，each drug group was given 7 days in advance，and the control group was given the same volume of normal saline.After the last administration，the model group and each drug group were intraperitoneally injected with 10 mL·kg-1 of 0.1% carbon tetrachloride (CCl4) olive oil.After 18 hours，orbital blood was collected，plasma was separated，and AST and ALT were detected；some liver tissues were taken to observe histopathological changes.Another Kunming mice were taken，and the grouping was the same as that of the administration.After the last administration，the mice were put into water to swim (25 ℃) according to 10% weight，and began timing.When the mouse sank into the water and no longer surfaced for 5 seconds，stop timing，calculate the swimming time of the mouse，take blood from the orbit，and detect the lactic acid content in the blood.The control group did not swim，and the lactic acid content was detected by blood sampling at the end of the experiment.Results Microscope and particle size analyzer showed that Astragalus pieces were crushed into small particles of different sizes，with particle size distribution ranging from 045 to 709 μm.Particles smaller than 80 μm are larger than 80%.Huangqi superfine powder (0.4~10 g·kg-1) can reduce liver necrosis and the increase of AST and ALT caused by 0.1% CCl4 (P<0.05).Huangqi superfine powder (0.4~10 g·kg-1) could significantly increase the swimming time of mice (P<0.05) and reduce the content of lactic acid in blood of mice (P<0.05).The liver protection and anti fatigue effects of 0.4 g·kg-1 Astragalus superfine powder are equivalent to those of 10 g·kg-1Astragalus pieces.The content of Astragaloside IV in Astragalus superfine powder was significantly increased by HPLC detection.Conclusion Compared with Astragalus pieces，Huangqi superfine powder has better liver protection and fatigue resistance，which may be related to the increased content of Astragaloside IV in Huangqi superfine powder.Abstract：Objective To observe the anti acute liver injury and anti fatigue effects of Huangqi ultrafine powder and the changes of astragaloside IV content，so as to provide scientific basis for the application of Huangqi ultrafine powder.Methods Astragalus pieces were crushed into ultra-fine powder by low-temperature ultra-fine pulverization technology.The microscopic characterization and particle size characterization were observed by microscope and laser particle sizer.The content of Astragaloside IV in Astragalus pieces and Huangqi ultra-fine powder was detected by HPLC.Kunming mice were randomly divided into control group (Con)，model group (M)，Huangqi decoction group (HQY，10 g·kg-1),Huangqi ultra-fine powder high-dose group (HQFH，10 g·kg-1)，Huangqi ultra-fine powder medium dose group (HQFM，2 g·kg-1)，Huangqi ultra-fine powder low-dose group (HQFL，0.4 g·kg-1).Except for the control group and the model group，each drug group was given 7 days in advance，and the control group was given the same volume of normal saline.After the last administration，the model group and each drug group were intraperitoneally injected with 10 mL·kg-1 of 0.1% carbon tetrachloride (CCl4) olive oil.After 18 hours，orbital blood was collected，plasma was separated，and AST and ALT were detected；some liver tissues were taken to observe histopathological changes.Another Kunming mice were taken，and the grouping was the same as that of the administration.After the last administration，the mice were put into water to swim (25 ℃) according to 10% weight，and began timing.When the mouse sank into the water and no longer surfaced for 5 seconds，stop timing，calculate the swimming time of the mouse，take blood from the orbit，and detect the lactic acid content in the blood.The control group did not swim，and the lactic acid content was detected by blood sampling at the end of the experiment.Results Microscope and particle size analyzer showed that Astragalus pieces were crushed into small particles of different sizes，with particle size distribution ranging from 045 to 709 μm.Particles smaller than 80 μm are larger than 80%.Huangqi superfine powder (0.4~10 g·kg-1) can reduce liver necrosis and the increase of AST and ALT caused by 0.1% CCl4 (P<0.05).Huangqi superfine powder (0.4~10 g·kg-1) could significantly increase the swimming time of mice (P<0.05) and reduce the content of lactic acid in blood of mice (P<0.05).The liver protection and anti fatigue effects of 0.4 g·kg-1 Astragalus superfine powder are equivalent to those of 10 g·kg-1Astragalus pieces.The content of Astragaloside IV in Astragalus superfine powder was significantly increased by HPLC detection.Conclusion Compared with Astragalus pieces，Huangqi superfine powder has better liver protection and fatigue resistance，which may be related to the increased content of Astragaloside IV in Huangqi superfine powder.
References:
[1]郭武汉,关二旗,卞科.超微粉碎技术应用研究进展[J].粮食与饲料工业，2015,12(5):38-40.
[2]郭妍婷,黄雪,陈曼,等.超微粉碎技术的应用研究进展[J].广东化工，2016,43(16):276-277.
[3]杨艳君,邹俊波,张小飞,等.超微粉碎技术在中药领域的研究进展[J].中草药,2019,50(23):5887-5891.
[4]李艺博,李娟,刘碧原,等.中药超微粉碎技术应用概况[J].中华中医药杂志,2020,35(9):4568-4570.
[5]钱珊珊,桂双英,杨满琴,等.中药超微粉碎技术的研究进展[J].陕西中医药大学学报,2019,42(3):136-140.
[6]赵凤存,刘怡菲,吴占军,等.中药超微粉碎技术的研究进展与思考[J].河北农业科学,2007,11(1):100-102.
[7]潘磊.中药材制成超微细粉的优点及应用[J].中国医药指南,2013,11(30):173-174.
[8]祝战科.新型中药材超细粉磨技术及应用[J].湖北农机化,2020（8）:144-148.
[9]国家药典委员会．中华人民共和国药典[S].一部.北京：中国医药科技出版社，2010:283.
[10]彭瑜,王永祥,秦红岩,等.基于网络药理学的黄芪治疗冠心病机制研究[J].中国中西医结合杂志,2020,40(8):935-940.
[11]马伟,李洪源,何旭,等.黄芪抗衰老作用研究进展[J].辽宁中医药大学学报,2020,22(4):21-24.
[12]靳贵林,陈正红,罗珍,等.红芪和黄芪的药理药效研究比较[J].西藏科技,2020(3):51-55.
[13]刘子菡,王喻淇,杨爱琳,等.基于网络药理学研究黄芪增强免疫功能的机制[J].中南药学,2020,18(10):1661-1667.
[14]殷园园,王秀莉,宋丹丹,等.中药微粉粒度的分析研究[J].河南科学，2014，32（10）：1992-1995.
[15]盛勇,刘彩兵,涂铭旌.超微粉碎技术在中药生产现代化中的应用优势及展望[J].中国粉体技术,2003,9(3):28-31.
[16]赵金花.中药细胞级粉碎技术在巴布剂中的应用价值分析[J].家庭医药·就医选药,2021(1):378-379.
[17]白照刚，张兴旺，李金华.中药细胞级粉碎技术在中药药剂中的应用效果观察[J].家庭医药·就医选药,2019(1):180.
[18]康桥.中药制剂中应用中药细胞级微粉碎技术分析[J].临床医药文献电子杂志,2018,5(99):165,168.
[19]张继平,杨海韵.超微粉碎技术是促进中药传统产业改造的有效途径[J].中药新药与临床药理,2003,14(3):209-211.
[20]孟琮,吴家胜,马越鸣.黄芪组分及主要成分保肝作用研究进展[J].中医学,2016,5(2):63-70.
[21]贾瑞红.黄芪的药理作用在运动领域的研究综述[J].辽宁体育科技,2016,38(1):53-55.
[22]朱尚文,魏佳,许勇镇,等.不同产地黄芪中黄芪多糖的含量及其抗疲劳、耐常压缺氧作用[J].陕西中医药大学学报,2016,39(1):86-89.
[23]周迎春.四氯化碳诱导的小鼠急性肝损伤模型的实验研究[J].黑龙江中医药,2018,47(1):90-91.
[24]付满玲,九红,袁叶飞.赶黄草总黄酮对四氯化碳所致小鼠急性化学性肝损伤的保护作用[J].现代食品科技,2021,37(6):50-56.
[25]谢磊,李由,刘新民,等.小鼠游泳耐力实验系统的建立与红景天抗疲劳作用的验证[J].中国比较医学杂志,2016,26(5):71-76.
[26]农生斌,梁华益,黄明丽,等.百香果果汁对衰老小鼠负重游泳及抗疲劳的实验研究[J].现代预防医学,2017,44(7):1281-1284.
[27]禹继洋,盛华刚.雄蜂蛹醇提物对小鼠抗运动性疲劳影响的实验研究[J].山东中医药大学学报,2021,45(3):384-387.
[28]朱凯峰.黄芪冰冻微粉对小鼠耐缺氧和抗疲劳作用的实验研究[J].黑龙江医药,2008,21(6):20-21.
[29]王谦,赖慧,陈立,等.高效液相色谱法测定3种剂型黄芪中黄芪甲苷和毛蕊异黄酮葡萄糖苷含量[J].中国药业,2019,28(6):23-26.
[30]李雅,杨永华,蔡光先.超微粉碎技术对黄芪药材主要化学成分提取率的影响[J].中成药,2008,30(2):229-231.
[31]杨泽锐,程翔燕,邓雯,等.黄芪破壁饮片煎煮、冲泡与传统饮片煎煮后5种有效成分溶出含量比较[J].中国药房,2017,28(19):2688-2692.
Memo
基金项目：北京市属医学科研院所公益发展改革试点项目（京医研2019-6）；北京市卫生系统高层次卫生技术人才培养计划（2015-3-101）