[1] QUAN Lina WANG LuWANG Jiawen YAO Hanbo LIU Hang,.Study on Extraction Technology and in Vitro Antioxidant Activity of Total Saponins from Polygonatum Multiflorum [J]. Modern Traditional Chinese Medicine, 2022, 1(05): 47-51. [doi: 10.13424/j.cnki.mtcm.2022.05.008]
Study on Extraction Technology and in Vitro Antioxidant Activity
of Total Saponins from Polygonatum Multiflorum
- Article number:
- 1672-0571(2022)05-0047-05
- Keywords:
- Key words:Polygonatum multiflorum; Extraction process; Total saponins; Response surface analysis; Antioxidant
- ZTFLH:
- R284.2
- Code:
- A
- Abstract:
- Abstract：Objective To optimize the extraction process of total saponins of Polygonatum multiflorum and study the antioxidant activity of total saponins of Polygonatum multiflorum.Methods Adopted Box Behnken design (BBD) response surface method，taking ethanol concentration，ethanol multiple and extraction time as influencing factors，and total saponin content as evaluation index，optimized the extraction process of total saponins of Polygonatum multiflorum；the antioxidant activity of total saponins was studied by 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and 2,2′-diazo-bis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS).Results The optimal extraction process of total saponins from Polygonatum multiflorum was 15 times 61% ethanol for 74 min.Polygonatum Polygonatum saponins have certain scavenging effects on DPPH and ABTS free radicals，with IC50 values of 91.90 respectively μg·mL-1 and 60.42 μg·mL-1.Conclusion The extraction process of total saponins of Polygonatum multiflorum optimized by response surface methodology is stable，reliable and predictable；the total saponins of Polygonatum multiflorum have certain antioxidant activity
References:
[1]国家药典委员会.中华人民共和国药典[S]一部.北京:中国医药科技出版社,2020：319.
[2]范佐旺,柯晓燕,陈靓雯,等.多花黄精的化学成分及药理研究进展[J].中医药信息,2020,37(5):119-126.
[3]陈辉,冯珊珊,孙彦君,等.3种药用黄精的化学成分及药理活性研究进展[J].中草药,2015,46(15):2329-2338.
[4]黄莺,徐维平,魏伟,等.黄精皂苷对慢性轻度不可预见性应激抑郁模型大鼠行为学及血清中微量元素的影响[J].安徽医科大学学报,2012,47(3):286-289.
[5]耿甄彦,徐维平,魏伟,等.黄精皂苷对抑郁模型小鼠行为及脑内单胺类神经递质的影响[J].中国新药杂志,2009,18(11):1023-1026.
[6]刘品华,冯亚娟,潘焦,等.蒸制时间对滇黄精抗氧化活性及吸附胆酸盐的影响[J].西南农业学报,2020,33(3):547-554.
[7]巫永华,张建萍,赵节昌,等.大孔树脂纯化黄精多酚及其抗氧化性与组成分析[J].农业工程学报,2020，36(1)：318-326.
[8]陈杨杨,胡慧玲,奉关妹,等.多花黄精炮制前后对游泳力竭小鼠抗疲劳抗氧化的影响[J].中药药理与临床,2021,37(2):92-96.
[9]李彦伟.超高压提取黄精多糖工艺优化、结构分析及抗氧化性研究[D].大连：大连理工大学,2019.
[10]何晓梅,李超,李永忠,等.不同产地黄精多糖含量测定及其体外抗氧化活性研究[J].皖西学院学报，2018,34(5):89-92.
[11]赵文莉,赵晔,Yiider Tseng.黄精药理作用研究进展[J].中草药，2018，49(18):4439-4445.
[12]吴良发,宁火华,岳翃熠,等.正交设计研究黄精炮制中美拉德反应产物的抗氧化活性[J].中国药师,2015(6):916-919.
[13]Guorong Du,Mingjun Li,Fengwang Ma,Dong Liang.Antioxidant capacity and the relationship with polyphenol and Vitamin C in Actinidia fruits[J].Food Chemistry,2008,113(2):557-562.
[14]白海娜,王振宇,刘瑞海,等.白藜芦醇与黑木耳多糖协同清除ABTS自由基活性的研究[J].现代食品科技,2014,30(3):64-68.
[15]范金波,蔡茜彤,冯叙桥,等.咖啡酸体外抗氧化活性的研究[J].中国食品学报,2015,15(3):65-73
[16]赵丽蓉,罗汉,相英龙,等.Box-Benhken响应面法优化微波提取黄精总皂苷的条件[J]中国现代中药,2018,20(8):1010-1015.
[17]王冬梅,朱玮,张存莉,等.卷叶黄精总皂苷含量测定方法及提取工艺研究[J].西北林学院学报,2006,21(3):107-110.
[18]包瑞敏,张智,杜亚飞,等.黄精总皂苷提取工艺优化及其对α-淀粉酶及α-葡萄糖苷酶抑制活性[J].食品工业科技,2020,41(16):163-168,175.
[19]祝凌丽,徐维平.黄精总皂苷和多糖的药理作用及其提取方法的研究进展[J].安徽医药,2009,13(7):719-722.
[20]尤新军,郭蕊,王琳,等.黄精总皂苷超声提取工艺研究[J].西北林学院学报,2010,25(3):163-166.
Memo
基金项目：陕西省科技厅重点研发计划项目社会发展领域（2017SF-335、2016SF-373）