[1] GAO Yang LI Ya QIN Kunming YANG Chao SUN Kanping LIU Qing ZHENG Linyu CAI Baochang JIN Junjie,.Comparative Analysis of Acute and Subchronic Toxicity of Raw and Processed Flos Lonicerae [J]. Modern Traditional Chinese Medicine, 2022, 1(06): 46-50. [doi: 10.13424/j.cnki.mtcm.2022.06.010]
Comparative Analysis of Acute and Subchronic Toxicity
of Raw and Processed Flos Lonicerae
- Article number:
- 1672-0571(2022)06-0046-05
- Keywords:
- Key words:Flos Lonicerae; Acute toxicity; Subchronic toxicity; Maximum tolerance; Maximum administration dosage
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the acute toxicity and subchronic toxicity of the raw and processed Flos Lonicerae.Methods The acute toxicity experiment was carried out by intragastric administration of the maximum tolerance dose (MTD) in mice. On the first day of the experiment, the mice were administered orally (i.g) for three times, and then observed for seven consecutive days. The weight, toxicity and death of the mice were recorded; The subchronic toxicity test was conducted by continuous intragastric administration in mice. The mice were administered orally once a day for 7 days, and the weight, toxicity and death of the mice were recorded. Results The MTD of the acute toxicity test mice of the raw and processed flos daturae was more than 120 g·kg-1, and the maximum tolerance multiple was 4800 times. Subchronic Toxicity Test of Flos Lonicerae Preparations:The daily dose of 20g mice was 276.8g, which was 184 times of the clinical dose, converted into the daily dose of 60kg person by the body surface area method. In the experiment, there was no poisoning and no death. The average weight of mice in each group showed an upward trend, and there was no significant difference. Conclusion The crude and processed products of Flos Lonicerae have no acute toxicity to mice, and the processed products of Flos Lonicerae have no sub chronic toxicity to mice. The processed products of Flos Lonicerae are safe to use in clinical dosage within 7 days, and the clinical dosage can be increased.
References:
［1］国家药典委员会.中华人民共和国药典［S］.北京：中国医药科技出版社，2020:279.
［2］上海市食品药品监督管理局.上海市中药饮片炮制规范:2008年版［M］.上海:上海科学技术出版社,2008.
［3］夏永严,李娅,秦昆明,等.正交试验优选制洋金花的炮制工艺研究［J］.中国民族民间医药，2021,30(9):58-62.
［4］黄诺嘉.毒性中药洋金花急性中毒6例报告［J］.中国药事，2007,21(2):141-142.
［5］黄弈涵,朱姝,李俊圻,等.有毒中药减毒方法探究［J］.中药材,2021,44(8):2028-2031.
［6］王佩佩,赵慧辉,李雪丽,等.浅谈中药毒性的控制［J］.中华中医药杂志，2018,33(2):433-435.
［7］姜华,李军,李欣,等.中药饮片现代炮制工艺研究概况［J］.中国民族民间医药，2020,29(8):46-50.
［8］彭新,陈晓辉,周浩,等.中药醋制入肝减毒、增效现代研究［J］.中华中医药学刊,2020,38(9):190-194.
［9］周志容,冯果,李玮,等.“汗渍法”炮制对了哥王中芫花素含量及其抗氧化能力的影响［J］.中国药房，2020,31(19):2320-2325.
［10］李江维,许润春,王鹏飞,等.去油制霜对美洲大蠊减毒增效的影响［J］.中成药，2017,39(7):1449-1454.
［11］林雨,佘亮，魏馨瑶，等.黄精炮制前后的化学成分变化及其减毒增效研究［J］.中药材，2021,44(6):1353-1359.
［12］刘佳,李强,郭莉,等.地黄提取物的急性毒性和亚慢性毒性试验研究［J］.中国畜牧兽医，2017,44(11):3372-3378.
［13］赵敏,黄俊明,谭剑斌,等.夏枯草的急性毒性和亚慢性毒性试验研究［J］.中国卫生检验杂志，2017,27(2):174-178.
［14］边林秀,张颖,田若涛,等.翅果仁的急性毒性和亚慢性毒性研究［J］.中国药物与临床，2020,20(19):3187-3190.
［15］谢小东,季露,孙钰博,等.天冬糖散急性毒性及亚慢性毒性研究［J］.中国畜牧兽医，2021,48(18):3097-3107.
［16］史芳芸,冯平,苏永霞,等.槐定碱急性与亚急性毒性研究［J］.动物医学进展，2020,41(5):44-50.
［17］何亮华,李建生,罗春煅.洋金花提取物对3种鲤科鱼类的急性毒性试验［J］.齐鲁渔业，2007,24(2):5-7.
［18］邱召娟,朱萱萱,倪文澎.洋金花伤膏的动物毒性实验研究［J］.中医药学刊，2006,24(10):1939-1940.
［19］舒波,李毅.炮制减毒原理对中药白花曼陀罗毒性成分含量的影响分析［J］.四川中医，2015,33(3):85-87.
［20］徐宁,孙民琴,张宏利,等.曼陀罗和多刺曼陀罗种子对小鼠的急性毒性研究［J］.检验检疫学刊，2015,25(2):8-10.
Memo
基金项目：江苏省科技成果转化专项资金项目（BA2018002）；南京市科技计划项目（201812021）；南京中医药大学自然基金项目(XZR2020100)