[1] ZHI Dongli LIU Huan HE Wei HU Yangcheng XU Yan,.Targeting AK1,CYP1B1,and MGAM with Maxing Shigan Decoction for Treatment of Community Acquired Pneumonia in Children [J]. Modern Traditional Chinese Medicine, 2023, (04): 83-90. [doi: 10.13424/j.cnki.mtcm.2023.04.017]
Targeting AK1,CYP1B1,and MGAM with Maxing Shigan Decoction for Treatment of Community Acquired Pneumonia in Children
- Article number:
- 1672-0571(2023)04-0083-08
- Keywords:
- Key words:Maxing Shigan decoction; Treatment; Community acquired pneumonia; Network pharmacology; Transcriptome
- ZTFLH:
- R246.4
- Code:
- A
- Abstract:
- Abstract：Objective To elucidate the main target of Maxing Shigan decoction in the treatment of children’s community acquired pneumonia through Transcriptome analysis combined with network pharmacology and experimental verification methods.Methods R 3.5.0 were analyzed and plotted using limma,factoextra,dplyr,tible,tidyr,and ggplot2 packages.The components and targets of Maxing Shigan decoction were obtained using TCMSP,TCMID,and PharmaMapper databases.Using Elisa for IL-6 and TNF-α Detection and Western Blot detection of AK1,CYP1B1,and MGAM.Results There were 335 differentially expressed genes between the community acquired pneumonia group and the control group;Maxing Shigan decoction can reduce the inflammatory damage of A549 cells;Maxing Shigan decoction can increase the level of AK1,reduce the levels of CYP1B1 and MGAM,and thus play a role in regulating community acquired pneumonia.Conclusion Maxing Shigan decoction can regulate the targets and inflammation levels related to community acquired pneumonia.
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Memo
基金项目：国家自然科学基金项目（82205190）