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[1] SHI Xinbo WANG Lei CHEN Jiaxin LIU Hongbo SONG Zhongxing LI Guolong DU Shengqi,.Study on Purification of Polysaccharides from the Stem of Huangjing [J]. Modern Traditional Chinese Medicine, 2023, (04): 101-104. [doi: 10.13424/j.cnki.mtcm.2023.04.020]

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Study on Purification of Polysaccharides from the Stem of Huangjing

Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN] Volumes & Issues: 2023 04 pagination: 101-104 Column: Publication date: 2023-07-20

Article number:: 1672-0571(2023)04-0101-04

Author(s):: SHI Xinbo¹ WANG Lei¹ CHEN Jiaxin¹ LIU Hongbo¹ SONG Zhongxing¹; 2 LI Guolong¹ DU Shengqi²; 1.Shaanxi University of Chinese Medicine/Shaanxi Province Ministry of Traditional Chinese Medicine Resources Industrialization Collaborative Innovation Center,Shaanxi Xianyang 712083,China;
2.Shaanxi Xingshengde Pharmaceutical Co.,Ltd.,Shaanxi Tongchuan 727031,China

Keywords:: Keywords:Huangjingstem; Polysaccharides; Refinement; Depigmentation; Deproteinization 黄精是百合科植物滇黄精Polygonatum kingianum Coll.et Hemsl.、黄精 Polygonatum sibiricum Red. 或多花黄精 Polygonatum cyrtonema Hua.

ZTFLH:: R282

DOI:: 10.13424/j.cnki.mtcm.2023.04.020

Code:: A

Abstract:: Abstract：Objective To refine the polysaccharides in the stem of Huangjing and provide a research basis for the comprehensive utilization of Huangjing stem resources.Methods Ethanol precipitation method was used to prepare crude polysaccharides from Huangjing stem;Using the decolorization rate and retention rate of polysaccharides as indicators,05% hydrogen peroxide and 2% activated carbon were used for decolorization research;With the protein removal rate and polysaccharide retention rate as indicators,the deproteinization of polysaccharide from the decolored rhizome of Polygonatum sibiricum was studied by using the Sevage method and Trichloroacetic acid method.Results The results showed that the crude polysaccharide from Huangjing stem was prepared using ethanol precipitation method,with a yield of 2.80% and a polysaccharide content of 11.33% in the crude polysaccharide;The decolorization effect of 0.5% hydrogen peroxide is better than that of 2% activated carbon,with an average decolorization rate of 78.88% and an average polysaccharide loss rate of 7.74%;Compared with the Sevage method,the protein removal rate of the Trichloroacetic acid method was 73.12%,and the polysaccharide loss rate was 9.34%.Conclusion The decolorization and deproteinization process with hydrogen peroxide and Trichloroacetic acid is feasible,simple and convenient,and can provide a reference for the resource utilization and development of non medicinal parts of Huangjing.

References:

[1]焦劼,陈黎明,孙瑞泽,等.不同产地黄精主要化学成分比较及主成分分析[J].中药材,2016,39(3)：519-522.
[2]李晓明.黄精化学成分及药理作用的研究[J].生物化工,2018,4(2)：138-139,145.
[3]李亚霖,周芳,曾婷,等.药用黄精化学成分与活性研究进展[J].中医药导报,2019,25(5)：86-89.
[4]张峰,高群,孔令雷,等.黄精多糖抗肿瘤作用的实验研究[J].中国实用医药,2007,2(21)：95-96.
[5]段华,王保奇,张跃文.黄精多糖对肝癌H22移植瘤小鼠的抑瘤作用及机制研究[J].中药新药与临床药理,2014,25(1)：5-7.
[6]Zhang H,Cai XT,Tian QH,et al.Microwave-assisted degradation of polysaccharide from Polygonatum sibiricum and antioxidant activity[J].Journal of Food Science,2019,84(4)：754-761.
[7]Li L,Thakur K,Liao BY,et al.Antioxidant and antimicrobial potential of polysaccharides sequentially extracted from Polygonatum cyrtonema Hua[J].International Journal of Biological Macromolecules,2018,114：317-323.
[8]贺维涛,年婧,赵重博.不同产地黄精多糖含量、多糖红外光谱及抗氧化活性研究[J].现代中医药,2022,42(6)：51-55.
[9]Zheng SY.Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model[J].Scientific Reports,2020,10：2246.
[10]王艺,彭国庆,江新泉,等.黄精多糖对糖尿病大鼠模型的保护机制研究[J].中医药导报,2017,23(2)：8-16.
[11]陆建美,闫鸿丽,王艳芳,等.滇黄精及其活性成分群对α-糖苷酶活性抑制作用研究[J].中国现代中药,2015,17(3)：200-203.
[12]Liu N,Dong ZH,Zhu XS,et al.Characterization and protective effect of Polygonatum sibiricum polysaccharide against cyclophosphamide-induced immunosuppression in Balb/c mice[J].International Journal of Biological Macromolecules,2018,107：796-802.
[13]宋丽艳,谷建梅,刘秀波.中药资源开发利用现状及可持续发展对策[J].中华中医药学刊,2009,27(1)：86-87.
[14]张丽,常青,王艳,等.响应面法优化提取黄精茎秆中总多糖、总皂苷、总黄酮的工艺研究[J].中医药导报,2019,25(8)：64-67.
[15]王结鑫,褚海清,赵雨,等.枸杞多糖的精制工艺优选[J].湖南中医杂志,2021,37(2)：150-152,158.
[16]童红,申刚.黄精药材中黄精多糖的含量测定[J].中国药业,2007,16(9)：20-21.
[17]屈小娟,田元,于吉臣,等.舒悠颗粒质量控制研究[J].现代中医药,2022,42(5)：39-46.
[18]贺维涛,赵重博,年婧.纤维素酶辅助提取陕产珠子参多糖工艺及抗氧化活性研究[J].陕西中医药大学学报,2022,45(6)：40-44.
[19]张丽华,叶世莉,刘思美,等.白及多糖的超滤陶瓷膜分离工艺研究[J].陕西中医药大学学报,2022,45(6)：34-39.
[20]李国龙,唐志书,宋忠兴,等.无机陶瓷膜微滤精制山茱萸水提液的实验研究[J].西北药学杂志,2015,30(3)：224-227.
[21]陈存武,张莉,王玉领,等.黄精多糖提取液的活性炭脱色研究[J].中国林副特产,2008(3)：1-3.
[22]梁引库,吴三桥.黄精多糖脱色和脱蛋白工艺研究[J].食品科技,2012,37(12)：166-169.
[23]贺立虎,周济铭,高鹏.黄精多糖脱蛋白工艺的研究[J].陕西农业科学,2008,54(3)：53-55.
[24]苑翼楠,周威.“药食同源”类食品国内外研究现状及展望[J].现代食品,2021(14)：118-121.
[25]杨冰峰,胥峰,李淑立,等.黄精化学成分·生理功能及产业发展研究进展[J].安徽农业科学,2021,49(11)：8-12.
[26]张从敬,陈子怡,牟欣尚,等.黄精产业发展现状与趋势[J].农业科技通讯,2021(3)：10-12.

Memo

基金项目：陕西省科技统筹创新工程计划项目（2016KTTSSF02-01）

Last Update: 2023-07-29