[1] ZHANG Lan LI NanGUO TingREN BoYANG Jianbing CUI Zheng,.Effect and Mechanism of Bushen Gengnian Formulaon the Function of Neuroendocrine Immune Networkin Rats with Perimenopausal Syndrome [J]. Modern Traditional Chinese Medicine, 2024, (05): 88-94. [doi: 10.13424/j.cnki.mtcm.2024.05.017]
Effect and Mechanism of Bushen Gengnian Formulaon the Function of Neuroendocrine Immune Networkin Rats with Perimenopausal Syndrome
- Article number:
- 1672-0571(2024)04-0087-10
- ZTFLH:
- R256.5
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the effects of the Bushen Gengnian formula on behavioral indicators，thymus，uterus，adrenal index，serum and hypothalamic NE and 5-HT content，and uterine HSP70 and ERα36 protein expression in rats with perimenopausal syndrome，and to explore its mechanism of action on the neuroendocrine immune network function of PMS rats.Methods The PMS rat model was replicated by ovariectomy.Rats with disrupted estrous cycles for 5 consecutive days were randomly divided into a model group，a western medicine group，and a large，medium，and small dose group of Bushen Gengnian formula，with 10 rats in each group.The control group consisted of 10 rats.The western medicine group was given a 1 mL/100 g dose of diethylstilbestrol aqueous solution by gavage，while the Bushen Gengnian formula three dose groups were given 0.015 mL·g-1、0.010 mL·g-1、0.005 mL·g-1 doses of decoction by gavage，respectively.The control group and model group were given 1 mL/100g of physiological saline by gavage for 45 consecutive days.After the intervention，the general condition of each group of rats was observed，and an open field experiment was conducted.The elevated cross maze experiment was used to test the behavioral changes of rats，and the thymus，uterus，and adrenal indices of each group of rats were calculated.The serum，hypothalamic NE，and 5-HT contents of each group of rats were detected by radioimmunoassay and chemiluminescence，respectively，Western blot was used to detect the expression of HSP70 and ERα36 proteins in the uterus of rats in each group.Results Compared with the model group，the three doses of Western medicine and Bushen Gengnian formula intervention can improve the general condition of rats；Compared with the model group，Western medicine and the Bushen Gengnian formula at high and medium doses significantly increased the central area residence time，total movement distance，arm opening residence time，and number of arm opening entries in rats (P<0.05)，increased thymus，uterus，and adrenal indices (P<0.05)，decreased serum and hypothalamic NE and 5-HT levels in rats (P<0.05)，and upregulated HSP70 and ERα36 protein expression in rats’uterus (P<0.05).Conclusion The Bushen Gengnian formula may enhance the thymus，uterus，and adrenal indices of PMS rats，reduce their serum and hypothalamic NE and 5-HT levels，upregulate their uterine HSP70 and ER α36 protein expression，comprehensively regulate the neuroendocrine immune network function of PMS rats，improve anxiety like behavior，and exert therapeutic effects on PMS.
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Memo
基金项目：国家自然科学基金资助项目（81603656）；陕西中医药大学附属医院院级项目（2020MS015）；陕西中医药大学青年基金项目（2015QN34）；全国第六批名老中医药专家学术经验继承项目（国中医药人教发［2017］29号）；全国名老中医药专家传承工作室建设项目（国中医药人教函［2022］75号）