[1] WANG Jiayong ZHU Xiaojun WANG Suxian GAO Wei YAN Ziqiang,.Mechanism of Polygonatum Sibiricum Polysaccharides RegulatingMacrophage Polarization and Inhibiting Migration andInvasion of Esophageal Cancer Cell Line Eca109 [J]. Modern Traditional Chinese Medicine, 2024, (06): 88-95. [doi: 10.13424/j.cnki.mtcm.2024.06.016]
Mechanism of Polygonatum Sibiricum Polysaccharides RegulatingMacrophage Polarization and Inhibiting Migration andInvasion of Esophageal Cancer Cell Line Eca109
- Article number:
- 1672-0571(2024)04-0079-04
- Keywords:
- Key words:Polygonatum sibiricum polysaccharide; Esophageal cancer; Tumor associated macrophage polarization; Attack; Transfer
- ZTFLH:
- R571
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the effect and mechanism of Polygonatum sibiricum polysaccharides (PSP) on macrophage polarization and invasion and migration of esophageal cancer cell line Eca109. Methods The Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of PSP on the survival rate of RAW264.7 macrophages; Interleukin-4 (IL-4) (20 ng·mL-1)/interleukin-13 (IL-13) (20 ng·mL-1) induced polarization of RAW264.7 cells into M2 macrophages, and PSP low, medium, and high (50, 100, 200 μg·mL-1) interventions were administered for 24 hours. Cell culture supernatant was collected to prepare conditioned medium. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA levels of M2 macrophage markers Arginase-1 (Arg-1), mannose receptor 1 (CD206), and Chitinase 3-like 3 (Ym-1); Use flow cytometry to detect the expression of M2 macrophage surface antigen CD206; The effect of scratch test conditions and culture medium on the migration ability of Eca109 cells; Transwell experiment detects the effect of conditioned medium on the invasive ability of Eca109 cells; Western blot assay was used to detect the expression of protein tyrosine kinase 3 (JAK3), phosphorylated protein tyrosine kinase 3 (p-JAK3), signal transducer and activator of transcription 6 (STAT6), and phosphorylated signal transducer and activator of transcription 6 (p-STAT6) proteins in cells. Results The results showed that PSP had no significant effect on the survival rate of RAW264.7 cells at concentrations below 200 μg·mL-1 (P≥0.05).PSP (50, 100, 200 μg·mL-1) showed a dose-dependent decrease in the proportion of CD206+ cells and reduced the levels of Arg-1, CD206, and Ym-1 mRNA (P<0.05). PSP macrophage conditioned medium significantly inhibits the invasion and migration ability of Eca109 cells. PSP (50, 100, 200 μg·mL-1) significantly reduced the protein expression ratios of p-JAK3/JAK3 and p-STAT6/STAT6 (P<0.05). Conclusion PSP can regulate macrophage M2 polarization and inhibit the invasion and migration of esophageal cancer cell line Eca109, and its mechanism of action may be related to the inhibition of the JAK3/STAT6 signaling pathway.
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Memo
基金项目：国家自然科学基金项目（81703882）；上海市药学会项目（2019-YY-09）