[1] Li Jie, Wang Yuan, et al. Effects of “ Three Olfactory Acupoints” Therapy on Learning and Memory Function and Nrf2 Expression in Vascular Dementia Rats [J]. Modern Traditional Chinese Medicine, 2020, (02): 29-32. [doi: 10.13424/j.cnki.mtcm.2020.02.007]
Effects of “ Three Olfactory Acupoints” Therapy on Learning and Memory Function and Nrf2 Expression in Vascular Dementia Rats
- Article number:
- 1672-0571(2020)02-0029-04
- Keywords:
- Three olfactory acupoints; Vascular dementia ( VD) ; Nrf2
- ZTFLH:
- R245
- Code:
- A
- Abstract:
- Objective To study the intervention effect of “ three olfactory acupoints” therapy on VD model rats and its relationship with hippocampal Nrf2 protein content. Methods Forty - five adult male SD rats were selected, weighing 200-220 g. They were randomly divided into three groups, namely the normal group, the model group ( VD model group) ,and “ three olfactory acupoints” group, with 15 in each. The model was made, and three olfactory acupoints were used for intervention treatment. Then, the water maze experiment was used to test the learning and memory ability of the rats, and the western blot method and ELISA were used to determine the hippocampal Nrf2 protein content. Results The results of the water maze test showed that, as for the average swimming distance and average escape latency, the escape latency of the model group was increased ( positioning navigation experiment) , the average time of the target platform quadrant occupied decreased, which was significantly different from the blank group ( P < 0. 01) ; after “ three olfactory acupoints” intervention, the escape latency of the rats was reduced, and the average time spent on the target platform quadrant wasincreased, which was significantly different from that of the model group ( P <0. 05) ; compared with the rats of the blank group The expression of Nrf2 in the model group increased significantly, with a significant difference ( P <0. 01) ; compared with the model group, the expression of Nrf2 increased in the “ three olfactory acupoints” group, which had a significant difference compared with the model group ( P <0. 05) . Conclusion “ Three olfactory acupoints” therapy can significantly improve the learning and memory ability of VD rats, and the mechanism is closely related to the activation of Nrf2 in the hippocampus of the brain.
References:
[1] Matthews FE, Arthur A, Barnes LE, et al. A two – decade comparison of prevalence of dementia in individuals aged 65 years and older from three geographical areas of England: results of the Cognitive Function and Ageing StudyⅠandⅡ[ J] . Lancet, 2013, 382( 9902) :1405 -1412.
[2]贾建平.临床痴呆病学[M].北京:北京大学医学出版社,2008:498.
[3] Tomohiro Morita, Asaka Higuchi, Akihiko Ozaki, at al.The possibility of vascular care for prevention of dementia[ J] . The Lancet, 2017, 389(65) :152 -153.
[4] Poornima Venkat, Michael Chopp, Alex Zacharek , at al.White matter damage and glymphatic dysfunction in a model of vascular dementia in rats with no prior vascular pathologies[ J] . Neurobiology of Aging, 2017, 50(2) : 96-106.
[5] Li H, He HJ, Wang ZX, et al. Rice protein suppresses ROS generation and stimulates antioxidant geneexpression via Nrf2 activation in adult rats[ J] . Gene, 2016, 585(2) : 256 ? 264.
[6] Khan NM, Haseeb A, Ansari MY, et al. Wogonin, a plant derived small molecule, exerts potent anti – inflammatory and chondroproprot - ective effects through the activation of ROS/ ERK/ Nrf2 signaling pathways in human Osteoarth - ritis chondrocytes[ J] . Free Radical Biology and Medicine, 2017, 106( 2) : 288 -301.
[7]刘智斌,牛文民,杨晓航,等.嗅三针治疗血管性痴呆的随机对照研究[J].针刺研究,2008,33(2):131-134.
[8]刘智斌,牛文民,杨晓航,等.嗅三针治疗血管性痴呆嗅觉功能障碍的临床研究[J].新中医,2011,43(3):99-100.
[9]刘智斌,牛文民,杨晓航,等.血管性痴呆之嗅觉功能障碍与嗅三针干预效应的临床研究[J].陕西中医学院学报,2010,33(1):44-45.
[10] Khan NM, Haseeb A, Ansari MY, et al. Wogonin, a plant derived small molecule, exerts potent anti – inflammatory and chondroproprot - ective effects through the activation of ROS/ ERK/ Nrf2 signaling pathways in human Osteoarth - ritis chondrocytes[ J] . Free Radical Biology and Medicine, 2017, 106( 2) : 288 -301.
[11] Kim J, Lim J, Kang BY, et al. Capillarisin augments anti - oxi - dative and anti - inflammatory responses by activating Nrf2/ HO - 1signaling[ J] . Neurochemistry International, 2017, 105( 5) :11 -20.
[12] Prasad S, Sajja RK, Kaisar MA, et al. Role of Nrf2 and protective effects of Metformin against t - obacco smoke -induced cerebrovascular toxicity [ J ] . Redox Biology,2017, 12( 2) : 58 -69.
[13] Zeng XP, Li XJ, Zhang QY, et al. Tert - Butylhydroquinone Protects Liver Against Ischemia/ Reerfusion Injury in Rats Through Nrf2 - Activating Anti - Oxidative Activity [ J] . Transplantati on Proceedings, 2017, 49 ( 2) : 366-372.
Memo
基金项目:国家自然科学基金面上项目(81674086);陕西中医药大学课题(2017SZKY-041) 通讯作者:王强,讲师,博士?E-mail:55307064@qq.com