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[1] SONG JianYUAN Minhui LIU Zhenghui CUI Binghui CHU Yupeng HUANG Yujuan HUI Jianping SHEN Shuwen YU Yong MENG Kaiqiang,.Effect of Jinguo Weikang Capsule on Wnt/Lgr5 Pathway Regulationof PLGC Rats Based on “Toxin Stasis Combined Obstruction” [J]. Modern Traditional Chinese Medicine, 2021, (04): 27-31. [doi: 10.13424/j.cnki.mtcm.2021.04.006]

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Effect of Jinguo Weikang Capsule on Wnt/Lgr5 Pathway Regulationof PLGC Rats Based on “Toxin Stasis Combined Obstruction”

Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN] Volumes & Issues: 2021 04 pagination: 27-31 Column: Publication date: 2023-11-20

Article number:: 1672-0571(2021)04-0022-05

Author(s):: SONG Jian¹YUAN Minhui¹ LIU Zhenghui¹ CUI Binghui¹ CHU Yupeng ¹HUANG Yujuan¹ HUI Jianping ¹ SHEN Shuwen¹ YU Yong² MENG Kaiqiang¹; 1.Shaanxi University of Traditional Chinese Medicine，Shaanxi Xianyang 712046，China;
2. Xi’an Hospital of Traditional Chinese Medicine,Xi’an 712021,China

Keywords:: “Toxin Stasis Combined Obstruction” theory; Jinguo Weikang Capsule; Precancerous lesions of gastric cancer; Rats; Wnt/Lgr5 signal pathway

ZTFLH:: R285.5

DOI:: 10.13424/j.cnki.mtcm.2021.04.006

Code:: A

Abstract:: Objective To study the molecular mechanism of the regulation of Wnt/Lgr5 signaling pathway in the model of gastric cancer rats based on the theory of “Toxin Stasis Combined Obstruction”.Methods SD male rats were randomly divided into blank group，model group，Jingguo Weikang Capsule (high，medium and low dose) treatment group and vitamin enzyme treatment group. We used MNNG combined with the comprehensive model making method of starvation and satiety to copy the pathological model of PLGC rats，and confirmed that the model was successfully copied and treated continuously for 6 weeks，The Wnt/Lgr5 signaling pathway gene and protein expression level in gastric mucosa of rats were detected by Western blot. Results: Compared with the model group，the expression levels of Wnt1 mRNA,β-catenin mRNA，Lgr5mRNA in the gastric mucosa epithelium of the rats in the treatment group was treated with Jinguo Weikang Capsule were significantly reduced (P<0.01).Conclusion Jinguo Weikang Capsule can effectively treat PLGC and reduce the risk of gastric cancer. It may be related to the reduction of Wnt1，β-catenin mRNA，Lgr5mRNA expression and gene transcription in gastric mucosa of rats by regulating Wnt/Lgr5 signaling pathway.

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Memo

基金项目：陕西省教育厅专项科学研究计划项目（18JK0225）；国家级大学生创新创业训练计划项目（201910716024）

Last Update: 2021-08-02