[1] ZHAO Jinli ZHONG Qi LI Changxiang XING Hongxia ZHAO Yan QU Huihua KONG Hui,.Therapeutic Effect and Mechanism of Compound Shenbai Decoction on ICR Mice with Chronic Eczema [J]. Modern Traditional Chinese Medicine, 2022, 1(02): 25-30. [doi: 10.13424/j.cnki.mtcm.2022.02.005]
Therapeutic Effect and Mechanism of Compound Shenbai Decoction on ICR Mice with Chronic Eczema
- Article number:
- 1672-0571(2022)02-0025-06
- Keywords:
- Key words：Compound Shenbai decoction; Effect evaluation; Mechanism of action; Chronic eczema; Eczema
- ZTFLH:
- R275.9
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the therapeutic effect and mechanism of Professor WANG Qingguo's empirical formula compound Shenbai decoction on ICR mice with chronic eczema.Methods Mice were randomly divided into 6 groups:blank group，model group，positive drug control group，low dose group，medium dose group and high dose group，with 7 mice in each group.The model group was sensitized with 2,4-dinitrochlorobenzene (DNCB) to establish the mouse ear chronic eczema model；The low，medium and high dose groups of compound Shenbai decoction were intervened with low，medium and high dose of compound Shenbai decoction (the concentrations were 0.16 g·mL-1，0.32 g·mL-1 and 0.64 g·mL-1 respectively)，and the positive drug group was intervened with compound dexamethasone acetate cream.The ear weight and thickness of each group of mice were observed，and the pathological changes were observed by HE method.The content of cytokines in serum and tissue was detected by ELISA.Results Compared with the model group，the high，medium and low dose groups of compound Shenbai decoction can reduce the degree of ear swelling of chronic eczema model mice，reduce the content of IL-4 and increase IFN-γ in serum of chronic eczema model mice；Reduce the contents of IL-4 and IL-17 in local tissues of mouse chronic eczema model.Conclusion Compound Shenbai decoction has obvious therapeutic effect on chronic eczema in mice，mainly by reducing inflammatory reaction and adjusting the imbalance of Th1/Th2 ratio，that is，through direct anti-inflammatory effect and indirect immune regulation.
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Memo
基金项目：第四批全国中医（临床、基础）优秀人才研修项目（国中医药人教发[2017]24 号）；中央高校基本科研业务费专项资金资助；横向课题（2180071720099）