[1] CHEN Ziying HUANG Jiaoli LIAO Yinghong CHEN JiatingSUN Xugao CHEN Xing XIAO Sui,.Optimization of Fufang Tiexian Process andStudy on Antibacterial Components [J]. Modern Traditional Chinese Medicine, 2024, (04): 100-108. [doi: 10.13424/j.cnki.mtcm.2024.04.019]
Optimization of Fufang Tiexian Process andStudy on Antibacterial Components
- Article number:
- 1672-0571(2024)04-0100-09
- Keywords:
- Key words:Acalypha australis Linn.; gallnut; Terminalia chebula Retz.; UPLC-Q-TOF-MS; Aeromonas hydrophila; Process optimization; Spectral effect relationship
- ZTFLH:
- R284.1
- Code:
- A
- Abstract:
- Abstract：Objective To optimize the optimal combination and extraction process of Fufang Tiexian based on its antibacterial efficacy, and to reveal the antibacterial substances of this traditional Chinese medicine compound.Methods The optimal combination of Fufang Tiexian was selected based on the efficacy of anti Pseudomonas aeruginosa as the evaluation index, and the content of gallic acid and antibacterial activity were used as the evaluation index. The extraction process of Fufang Tiexian was optimized through single factor experiments and response surface methodology; Establish chemical fingerprints of Fufang Tiexian with different extraction processes and identify “common peaks”. Through correlation analysis, establish a “spectrum effect relationship” between the fingerprint and antibacterial efficacy, and use UPLC-Q-TOF-MS technology for component identification to identify the key components that inhibit Aeromonas hydrophila. Results The optimal combination of Fufang Tiexian was found to be Acalypha australis Linn.∶gallnut∶Terminalia chebula Retz.=2∶2∶1 (w/w/w), with a solid-liquid ratio of 1∶25. The optimal extraction process was to soak for 22 minutes and then boil for 60 minutes. Under these conditions, the extract of Fufang Tiexian had the best inhibitory effect on Aeromonas hydrophila; Four substances highly positively correlated with antibacterial activity were identified from the fingerprint of Fufang Tiexian∶chebulic acid＞chebulic acid＞gallic acid 4-O-(6-O-gallic acid) glucoside＞gallic acid (correlation coefficient between 0.499 and 0.846). Conclusion Acalypha australis Linn, Chinese gallnut, and Terminalia have significant inhibitory activity against Pseudomonas aeruginosa under the optimal combination and process conditions. Through the “spectrum effect relationship”, potential antibacterial substances in this traditional Chinese medicine compound can be quickly identified, providing scientific basis for the further development of Fufang Tiexian as a drug for the prevention and treatment of Pseudomonas aeruginosa.
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Memo
基金项目：广东省自然科学基金面上项目（2021A1515012153）