[1] ZHAO Qiufang WANG Xuemei LI Qiang ZHANG Wei,.Exploration of the Effect of Saponins from Tribulus Terrestrison Apoptosis of Graves’ Ophthalmopathy Cells Basedon the PI3K-AKT Signaling Pathway [J]. Modern Traditional Chinese Medicine, 2024, (06): 96-101. [doi: 10.13424/j.cnki.mtcm.2024.06.017]
Exploration of the Effect of Saponins from Tribulus Terrestrison Apoptosis of Graves’ Ophthalmopathy Cells Basedon the PI3K-AKT Signaling Pathway
- Article number:
- 1672-0571(2024)04-0087-10
- Keywords:
- Key words:Saponins from Tribulus terrestris; PI3K; AKT; Graves’ ophthalmopathy; Nthy-ori-3-1
- ZTFLH:
- R581.9
- Code:
- A
- Abstract:
- Abstract：Objective To investigate the effect and molecular mechanism of saponins from Tribulus terrestris on apoptosis of human thyroid normal cells Nthy-ori-3-1 in an in vitro cell model of Graves’ disease. Methods M22 induced human thyroid follicular epithelial cell line (Nthy-ori-3-1) to establish an in vitro Graves cell model, with control group, model group, and Tribulus terrestris saponin treatment group (5, 10, 20 ng·mL-1). CCK-8 evaluation of the proliferation ability of Nthy-ori-3-1 cells in each group; ELISA evaluation of cAMP changes in cell culture media of each group; TUNEL evaluation of apoptosis in each group of cells; Immunofluorescence evaluation of changes in Bcl-2, Bax, and Caspase-3 in each group of cells; Western Blot was used to evaluate changes in PI3K, AKT, p-PI3K, and p-AKT proteins. Results The high concentration of saponins from Tribulus terrestris promotes apoptosis of Nthy-ori-3-1 cells and inhibits their proliferation; High concentration saponins from Tribulus terrestris inhibit cAMP expression induced by M22 in Nthy-ori-3-1 cells; High concentration saponins from Tribulus terrestris promote the expression of Bax and Caspase-3, while inhibiting the expression of Bcl-2; High concentration saponins from Tribulus terrestris inhibit the expression of p-PI3K and p-AKT. Conclusion High concentration saponins from Tribulus terrestris inhibit the PI3K-AKT signaling pathway and promote apoptosis in Graves’ cells.
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Memo
基金项目：陕西省重点研发计划项目（2017SF-350）