[1] DU Xu ZHANG Rongchao LIU Juan ZHANG Haobin LI Yujie HU Wenjing,.Effects of Inverse Moxibustion on PERK and ATF4 in Hypothalamic Endoplasmic Reticulum Stress Pathway in Sub-health Rats [J]. Modern Traditional Chinese Medicine, 2022, 1(02): 31-35. [doi: 10.13424/j.cnki.mtcm.2022.02.006]
Effects of Inverse Moxibustion on PERK and ATF4 in Hypothalamic Endoplasmic Reticulum Stress Pathway in Sub-health Rats
- Article number:
- 1672-0571(2022)02-0031-05
- Keywords:
- Key words：Inverse moxibustion; Sub-health status; Hypothalamus; PERK; ATF4
- ZTFLH:
- R245.8
- Code:
- A
- Abstract:
- Abstract：Objective To explore some mechanisms of inverse moxibustion in preventing sub-health in rats from the stress pathways PERK and ATF4 of hypothalamic endoplasmic reticulum.Methods Clean healthy male SD rats were randomly divided into blank group，model group and inverse moxibustion group.In the inverse moxibustion group，Baihui，Zusanli and Shenshu points were selected for inverse moxibustion (moxibustion pretreatment) for 21 days，and then the sub-health model of rats was prepared by restraint stress combined with swimming exercise (21 days)；The blank group did not receive any treatment.The rats in each group were subjected to open field experiment on the 23rd and 45th days；On the 45th day，the changes of hypothalamic protein kinase like Er kinase (PERK) and transcription activator 4 (ATF4) were measured by immunohistochemistry (SABC).Results After the 23rd day，the open field test score of the reverse moxibustion group was significantly different from that of the model group (P<0.01)，and there was no significant difference from that of the blank group (P>0.05).After the 45th day，the open field test score of the model group was significantly lower than that of the blank group (P<0.01)；The score of inverse moxibustion group was higher than that of model group and lower than that of blank group (P<0.01).After the 45th day，compared with the blank group，the content of PERK in model group and inverse moxibustion group increased significantly，there was a very significant difference in model group (P<0.01)，and there was a significant difference in inverse moxibustion group (P<0.05)；Compared with the model group，the inverse moxibustion group was lower，with significant difference (P<0.05).Compared with the blank group，the content of ATF4 in model group and inverse moxibustion group was also significantly higher (P<0.05).Compared with the model group，the content of ATF4 in inverse moxibustion group was also lower (P<0.05).Conclusion The prevention of sub-health state by inverse moxibustion may be related to the regulation of hypothalamic endoplasmic reticulum stress pathway PERK and ATF4.
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Memo
基金项目：陕西省中医药管理局基础类研究项目（2019-ZZ-JC019）；陕西中医药大学博士启动科研项目（17102031829）