[1]孙理军 孙瑜嬬 姜梅 李翠娟 张琪 孙耀光.补肾中药对肾虚质大鼠海马区 ＥＲＫ１表达的影响[J].现代中医药,2018,(06):144-148.[doi:10.13424/j.cnki.mtcm.2018.06.047]
Sun Lijun Sun Yuru Jiang Mei Li Cuijuan Zhang Qi Sun Yaoguang.Effect of Chinese Herbal Medicine for Invigorating Kidney on
Expression of ERK1 in Hippocampus of Rats with Kidney Deficiency[J].Modern Traditional Chinese Medicine,2018,(06):144-148.[doi:10.13424/j.cnki.mtcm.2018.06.047]
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补肾中药对肾虚质大鼠海马区 ＥＲＫ１表达的影响(
)
《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
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- 期数:
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2018年06期
- 页码:
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144-148
- 栏目:
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药物研究
- 出版日期:
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2018-11-18
文章信息/Info
- Title:
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Effect of Chinese Herbal Medicine for Invigorating Kidney on
Expression of ERK1 in Hippocampus of Rats with Kidney Deficiency
- 文章编号:
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1672－0571(2018)06－0144－05
- 作者:
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孙理军 1 孙瑜嬬 1 姜梅 2 李翠娟 1 张琪 1 孙耀光 1
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1.陕西中医药大学，陕西 咸阳 ７１２０４６；2.陕西中医药大学研究生，陕西 咸阳 ７１２０４６
- Author(s):
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Sun Lijun 1 Sun Yuru 1 Jiang Mei 2 Li Cuijuan 1 Zhang Qi 1 Sun Yaoguang 1
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1． Shaanxi University of Chinese Medicine，Xianyang China，712046;
2． Postgraduate School of Shaanxi University of Chinese Medicine，Xianyang China，712046
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- 关键词:
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补肾中药; 肾藏志; 肾虚体质; 学习记忆; ERK1
- Keywords:
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kidney － invigorating herbs; kidney storing will; constitution of kidney deficiency; learning and memo-
ry; ERKI
- 分类号:
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R285. 5
- DOI:
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10.13424/j.cnki.mtcm.2018.06.047
- 文献标志码:
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A
- 摘要:
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目的 观察补肾中药对肾虚质大鼠学习记忆能力及海马区 ＥＲＫ１蛋白表达的影响，从突触可塑性角 度探讨其改善肾虚体质学习记忆能力的内在分子机制。方法 采用“猫吓鼠”造模法构建先天不足加后天恐吓型 肾虚质大鼠模型，分为模型组、补肾组、补心组、疏肝组 ４组，补肾组、补心组、疏肝组分别予以六味地黄丸、天王 补心丹、逍遥丸，空白组产自正常孕鼠。Ｍｏｒｒｉｓ水迷宫实验检测各组大鼠学习记忆能力；称取体重、脑重比较生 长发育情况；免疫组化法检测海马 ＥＲＫ１阳性细胞表达。结果 与空白组比较，模型组总路程显著增加，３０ｓ内目
- Abstract:
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To observe the effect of kidney － invigorating herbs on learning and memory a-
bility and expression of ERK1 protein in hippocampus of rats with kidney － deficiency syndrome，and to
explore the intrinsic molecular mechanism of improving learning and memory ability of kidney － deficien-
cy syndrome from the perspective of synaptic plasticity． Method: The rat model of congenital insufficiency
and acquired intimidation of kidney deficiency was established by "cat scare rat" model． The rats were
divided into four groups: model group，kidney － invigorating group，heart － tonifying group and liver －
soothing group． The kidney － invigorating group，heart － tonifying group and liver － soothing group were
given Liuwei Dihuang Pills，Tianwang Buxin Bolus and Xiaoyao pills respectively． The blank group wasproduced from normal pregnant rats． Morris water maze test was used to test the learning and memory a-
bilities of rats in each group． The body weight and brain weight were used to compare the growth and
development． The expression of ERK1 positive cells in hippocampus was detected by immunohistochem-
istry． Result: Compared with the blank group，the total distance of the model group increased significant-
ly． The target area retention time，body weight，brain weight and ERK1 positive expression in the hip-
pocampus were significantly reduced within 30s． Compared with the model group，the total distance of
the kidney － invigorating group，heart － tonifying group and liver － soothing group were all significantly
decreased，the target area retention time，body weight，brain weight and hippocampus ERK1 positive
expression increased significantly within 30s; Compared with the heart － tonifying group and liver －
soothing group，the total distance of kidney － invigorating group decreased significantly，and the reten-
tion time，body weight，brain weight and positive expression of ERK1 in hippocampus increased signifi-
cantly in 30 seconds． Conclusion: Kidney － invigorating herbs can improve the learning and memory of
rats with kidney deficiency by increasing the expression of learning memory protein ERK1 in hippocam-
pus; the expression of ERK1 in hippocampus is related to the theory of“kidney storing will”．
参考文献/References:
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[1０]施新猷．现代医学实验动物学［Ｍ]．北京：人民军医出 版社，２０００：３３２－３３５．
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[1３]ＳｉｌｖａＡＪ，ＦｒａｎｋｌａｎｄＰＷ，ＭａｒｏｗｉｔｚＺ，ｅｔａｌ．Ａｍｏｕｓｅｍｏｄｅｌ ｆｏｒｔｈｅｌｅａｒｎｉｎｇａｎｄｍｅｍｏｒｙｄｅｆｉｃｉｔｓａｓｓｏｃｉａｔｅｄｗｉｔｈｎｅｕｒｏ ｆｉｂｒｏｍａｔｏｓｉｓｔｙｐｅＩ［Ｊ]．ＮａｔＧｅｎｅｔ，１９９７，１５（３）：２８１ －２８４．
[1４]刘永惠，李少为，郑清莲．补肾健脑方对血管性痴呆大 鼠乙酰胆碱和海马区 ＥＲＫ１和 ＥＲＫ２表达的影响
备注/Memo
- 备注/Memo:
-
收稿日期:2018 －09 －10 编辑:文颖娟*
基金项目:陕西省科技厅社发项目(2018SF －312)
更新日期/Last Update:
2019-12-09