[1]郭建平 夏勤 顿文亮 吴?岚　黄书亮/sup>.槐花散治疗溃疡性结肠炎肠风下血证作用机制研究[J].现代中医药,2020,(05):009-14/21.[doi:10.13424/j.cnki.mtcm.2020.05.003]
　Guo Jianping,Xia Qin,Dun Wenliang,et al.A Study on the Effect and Mechanism of Huaihua Powder in the Treatment of Ulcerative Colitis by Emulating the Intestinal Subwind Blood Syndrome[J].Modern Traditional Chinese Medicine,2020,(05):009-14/21.[doi:10.13424/j.cnki.mtcm.2020.05.003]
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槐花散治疗溃疡性结肠炎肠风下血证作用机制研究()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
009-14/21
栏目:
出版日期:
2023-11-20

文章信息/Info

Title:
A Study on the Effect and Mechanism of Huaihua Powder in the Treatment of Ulcerative Colitis by Emulating the Intestinal Subwind Blood Syndrome
文章编号:
1672-0571(2020)05-0009-07
作者:
郭建平1 夏勤1 顿文亮1 吴?岚2　黄书亮3/sup>
1.南京市中医院药剂科,江苏南京210001;
2.南京市中医院肛肠科,江苏南京210001;
3.南京市中医院病理科,江苏南京210001
Author(s):
Guo Jianping;  Xia Qin;  Dun Wenliang;  Wu Kunlan;  Huang Shuliang
1.Department of Pharmacy in Nanjing Hospital of Traditional Chinese Medicine, Nanjing Jiangsu 210001;
2.Department of Proctology in Nanjing Hospital of Traditional Chinese Medicine, Nanjing Jiangsu 210001;
3.Department of pathology in Nanjing Hospital of Traditional Chinese Medicine, Nanjing Jiangsu 210001
关键词:
肠风下血; 槐花散; 溃疡性结肠炎; TNF-α; MPO
Keywords:
intestinal subwind blood syndrome;  Huaihua powder;  ulcerative colitis;  TNF - α;  MPO
分类号:
R574.62
DOI:
10.13424/j.cnki.mtcm.2020.05.003
文献标志码:
A
摘要:
建立溃疡性结肠炎大鼠模型，研究槐花散治疗肠风下血证，初步揭示槐花散治疗肠风下血证的机制。方法通过高效液相色谱法对槐花散水煎液及乙酸乙酯各萃取各部位芦丁、槲皮苷、柚皮苷和新橙皮苷进行定量，对其质量进行控制，并以５％右旋葡聚糖硫酸钠建立溃疡性结肠炎大鼠模型，以ＤＡＩ评分、炎症因子和炎性细胞浸润等情况评价各部位的治疗作用。结果槐花散合煎液、乙酸乙酯层及水层部位可显著降低血清中肿瘤坏死因子（ＴＮＦ－α）、髓过氧化酶（ＭＰＯ）炎症因子水平；显著降低ＤＡＩ分值；组织切片结果显示给药后可缓解炎症细胞浸润，且合煎液抗炎、抗溃疡和止血作用最强。结论肠风下血证经典方槐花散可有效治疗溃疡性结肠炎大鼠，主要通过减轻炎症细胞浸润，降低炎症水平来发挥作用；溃疡性结肠炎大鼠模型也许可作为肠风下血证的研究模型用于相关经典处方的进一步开发研究。
Abstract:
Objective A rat model of ulcerative colitis is established for the study of Huaihua powder ( sophora japonica) in the treatment of intestinal subwind blood syndrome to preliminarily reveal its mechanism. Methods HPLC( high performance liquid chromatography) is used to quantify the Huaihua powder decoction and the extracted parts from rutin, quercetin, naringin and neohesperidin by ethyl acetate and control its quality. In addition, UC rat model is established by 5% of dextran sodium sulfate and evaluate the the therapeutic effect of each part with DAI scores, inflammatory factors and nflammatory cell infiltration. Result The levels of tumor necrosis factor ( TNF - α) and medullary peroxidase ( MPO) inflammatory factors in serum are significantly reduced in the decoction, ethyl acetate and water layer and the DAI score is significantly reduced. The results of tissue sections indicate that the drug could relieve inflammatory cell infiltration, and the anti - inflammatory, anti - ulcer and hemostatic effects become the strongest combining with the decoction. Conclusion The classical prescription of Huaihua powder can effectively treat the rats with ulcerative colitis by reducing the infiltration of inflammatory cells and the level of inflammation. The rat model of ulcerative colitis may also be used as a model of subintestinal wind blood syndrome for the further development of relevant classic prescriptions.

参考文献/References:

[1]杨小波,罗云坚.古代中医对溃疡性结肠炎相关认识的启示[J].新中医,2007,39(5):103.
[2]温明银,李荆玉,毕世梅.“肠风下血”证治漫谈[J].黑龙江中医药,1997,40(2):33-34.
[3]王高峰,黄天生,朱生睴.朱生睴辨证治疗溃疡性结肠炎经验[J].中医杂志,2010,51(S1):100-101.
[4]中国中西医结合学会消化系统疾病专业委员会.溃疡性结肠炎中西医结合诊疗指南(草案)[J].中国中西医结合消化杂志,2011,19(1):61-65.
[5]王琼,李信平.槐花汤加味治疗溃疡性结肠炎大出血验案[J].中外医疗,2008,27(25):88.
[6]李惠,原桂东,金亚宏,等.槐花饮片及其提取物止血作用的实验研究[J].中国中西医结合杂志,2004,24(11):1007-1009.
[7]丁航,刘慧明,梁统,等.侧柏叶中黄酮类化合物H2O2诱导的人红细胞氧化作用的影响[J].实用临床医学,2003,4(3):23-24.
[8]吴婷,丁安伟,张丽.荆芥现代研究概况[J].江苏中医药,2004,25(10):64-67.
[9]梁统,覃燕梅,梁念慈.侧柏叶提取物抗炎作用的研究[J].中国药科大学学报,2001,32(3):66-68.
[10]张改先.荆芥穗的化学成分?药理作用及临床应用[J].山西职工医学院学报,2011,21(1):57-58.
[11]潘宪伟,赵余庆.侧柏叶和果实中黄酮类和萜类物质的现代药学研究进展[J].中草药,2012,43(8):1641-1646.

备注/Memo

备注/Memo:
基金项目：江苏省协同创新项目（Ｈ１４０２）
更新日期/Last Update: 2020-09-17