[1]朱瑞康李江李文韬 颜延凤.前胡汤对急性支气管炎大鼠气道神经源性炎症改善作用机制研究[J].现代中医药,2021,(03):039-46.[doi:10.13424/j.cnki.mtcm.2021.03.009]
　ZHURuikang,LIJiang,LIWentao,et al.MechanismofQianhuDecoctiononAirwayNeurogenicInflammationinRatswithAcuteBronchitis[J].Modern Traditional Chinese Medicine,2021,(03):039-46.[doi:10.13424/j.cnki.mtcm.2021.03.009]
点击复制

前胡汤对急性支气管炎大鼠气道神经源性炎症改善作用机制研究
分享到：

《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
039-46
栏目:
出版日期:
2021-05-20

文章信息/Info

Title:
MechanismofQianhuDecoctiononAirwayNeurogenicInflammationinRatswithAcuteBronchitis
文章编号:
1672-0571(2021)03-0039-08
作者:
朱瑞康1李江1李文韬1 颜延凤2
１．南京中医药大学，江苏 南京 ２１００２３；
２．南京中医药大学附属南京中西医结合医院，江苏 南京 ２１００１４
Author(s):
ZHURuikang1; LIJiang1; LIWentao1; YANYanfeng2
1.NanjingUniversityofTraditionalChineseMedicine,Nanjing210023,China; 2.NanjingIntegratedTraditionalChineseandWesternMedicineHospitalAffiliatedto NanjingUniversityofTraditionalChineseMedicine,Nanjing210014,China
关键词:
急性支气管炎; 前胡汤; 神经源性炎症; Ｐ物质; 信号通路
Keywords:
Acutebronchitis; QianhuDecoction; Neurogenicinflammation; SubstanceP; Signalpathway
分类号:
R965
DOI:
10.13424/j.cnki.mtcm.2021.03.009
文献标志码:
A
摘要:
目的 探讨临床验方前胡汤对支气管炎神经源性炎症的相关调控机制?方法 采用烟熏法建立大鼠 急性支气管炎模型,将大鼠随机分为对照组?模型组?急支糖浆组?惠菲宁组及前胡汤高?低剂量组?检测大鼠血 清 P物质(SP)?神经激肽 A(NKA)?神经激肽 B(NKB)?降钙素基因相关肽(CGRP)?神经生长因子(NGF)水平, 测定支气管肺泡灌洗液中神经源性炎症因子和肺组织中 SP和 NGF水平,肺组织中 SP受体神经激肽受体 l(NK -1)?辣椒素受体亚型 1(VR1)及 p38MAPK的 mRNA和蛋白表达水平?结果 模型组大鼠神经源性炎症物质水 平显著升高,前胡汤可明显抑制模型大鼠体内神经源性炎症物质水平,抑制作用具有一定的剂量相关性(p< 0.05)?造模可以明显增加肺组织中 NK-1及 p38MAPK的 mRNA和蛋白表达,但对于 VR1的 mRNA和蛋白表 达未见显著影响?前胡汤对 NK-1和 p38MAPK的表达量有明显抑制作用(p<0.05),对 VR1的 mRNA和蛋白 表达均未见明显影响?结论 急性支气管炎大鼠伴有神经源性炎症物质水平显著升高,前胡汤对气道神经源性 炎症具有较好的改善作用,其作用机理与调节 NGF/SP/p38MAPK信号通路有关?
Abstract:
Objective:ToexploretheregulatorymechanismofQianhuDecoctiononneurogenicinflammationofbronchitis.Methods:Theratmodelofacutebronchitiswasestablishedbysmoking.Theratswererandomlydividedintocontrolgroup,modelgroup,Jizhisyrupgroup,Huifeininggroup,QianhuDecoctionhigh-doseandlow-dosegroups.ThelevelsofsubstanceP(SP),neurokininA(NKA),neurokininB(NKB),calcitoningene-relatedpeptide(CGRP)andnervegrowthfactor(NGF)inserum,thelevelsofneurogenicinflammatoryfactorsinbronchoalveolarlavagefluid,SPandNGFinlungtissue,SPreceptorneurokininreceptorL(NK-1),capsaicinreceptorsubtype1(VR1)andp38MAPKmRNAandproteinoflungtissueweremeasured.Results:Thelevelsofneurogenicinflammatorysubstancesinthemodelgroupweresignificantlyinreased.QianhuDecoctioncouldsignificantlyinhibitthelevelsofneurogenicinflammatorysubstancesinthemodelrats,andtheinhibitioneffectshadacertaindosecorrelation(p<0.05).ThemRNAandproteinexpressionofNK-1andp38MAPKinlungtissueweresignificantlyincreased,butthemRNAandproteinexpressionofVR1werenotsignificantlyaffected.QianhuDecoctionsignificantlyinhibitedtheexpressionofNK-1andp38MAPK(p<0.05),buthadnosignificanteffectsonthemRNAandproteinexpressionofVR1.Conclusion:Thelevelsofneurogenicinflammatorysubstancesinratswithacutebronchitisaresignificantlyincreased.QianhuDecoctioncanimproveairwayneurogenicinflammation,anditsmechanismisrelatedtotheregulationofNGF/SP/p38MAPKsignalingpathway

参考文献/References:

[1]GuoLJ,ZhaoA,ChenRJ,etal.AssociationbetweenAmbientAirPollutionandOutpatientVisitsforAcuteBronchitisinaChineseCity[J].BiomedicalandEnvironmentalSciences,2014,27(11):833-840.
[2]SinghM,KoyfmanA.AreAntibioticsEffectiveintheTreatmentofAcuteBronchitis?[J].AnnalsofEmergency Medicine,2015,65(5):566-567.
[3]各廷秋,张念志,张润.急性气管 -支气管炎中西医 治疗 进 展 [J].辽 宁 中 医 药 大 学 学 报,2016(11): 65-68.
[4]BarnesPJ.Neurogenicinflammationintheairways[J].RespirationPhysiology,2001,125(1-2):145-154.
[5]叶新民,刘春丽,钟南山,等.感染后咳嗽及其神经源 性炎症机制研究进展[J].国际呼吸杂志,2010,30 (23):317-320.
[6]李文韬,颜延凤.自拟前胡汤治疗咳喘肺热喘嗽证探析[J].广西中医药,2020,43(1):37-38.
[7]彭德祺.自拟前胡汤的临床应用[J].四川中医,2007, 25(9):119-120.
[8]袁颖,高静琰,薛明明,等.疏风解毒胶囊对于急性支 气管炎大鼠模型的保护作用[J].中华中医药杂志, 2017,(32):278-281.
[9]孙涛,彭成,谢晓芳,等.川贝母止嗽颗粒对大鼠急性支 气管炎的影响[J].中药药理与临床,2013,29(3): 150-153.
[10]TakedaM,MiyakeM,MutoT,etal.ProliferationofsensoryCfibersandsubsequentneurogenicinflammationinratairwayinducedbyinhaledlipopolysaccharide[J].NeuroToxicology,2011,32(6):954-962.
[11]LeeLY.RespiratorysensationsevokedbyactivationofbronchopulmonaryC-fibers[J].RespiratoryPhysiology&Neurobiology,2009,167(1):26-35.
[12]LacroixJS,LandisBN.Neurogenicinflammationoftheupperairwaymucosa.[J].Rhinology,2008,46(3):163-165.
[13]YangH,LiSZ.TransientReceptorPotentialAnkyrin1(TRPA1)ChannelandNeurogenicInflammationinPathogenesisofAsthma[J].MedicalenceMonitorInternationalMedicalJournalofExperimental&ClinicalResearch,2016,(22):2917-2923.
[14]GronebergDA,QuarcooD,FrossardN,etal.Neurogenicmechanismsinbronchialinflammatorydiseases[J].Allergy(Oxford),2004,59(11):1139-1152.
[15]MurakiM,IwanagaT,HaraguchiR,etal.Continuedinhalationoflidocainesuppressesantigen-inducedairwayhyperreactivityandairwayinflammationinovalbumin-sensitizedguineapigs[J].InternationalImmunopharmacology,2008,8(5):725-731.
[16]BozkurtTE,LarssonO,AdnerM.StimulationofcannabinoidCB1receptorspreventsnerve-mediatedairwayhyperreactivityinNGF-inducedinflammationinmouseairways[J].EuropeanJournalofPharmacology,2016,776:132-138.
[17]MutohT,BonhamAC,JoadJP.SubstancePinthenucleusofthesolitarytractaugmentsbronchopulmonaryCfiberreflexoutput.[J].AmericanJournalofPhysiologRegulatoryIntegrative&ComparativePhysiology,2000,279(4):R1215-1223.
[18]OConnorTM,OConnellJ,OBrienDI,etal.TheroleofsubstancePininflammatorydisease.JCellPhysiol,2004,201(2):167-180.
[19]韦杰.气道神经源性炎症介质在感染后慢性咳嗽发病机制中的作用[J].白求恩医学杂志,2012,10(5):373-374.
[20]孟 倩,宋 春 梅,陈 丽 军.清 金 化 痰 汤 调 节 ERK/p38MAPK信号通路改善哮喘大鼠模型气道炎症的研究[J].四川中医,2019,37(10):51-55.
[21]Payan,DonaldG.SubstanceP:AModulatorofNeuroendocrine-ImmuneFunction[J].HospitalPractice,1989,24(2):67-80.
[22]Mezey,Eva,Toth,etal.DistributionofmRNAforvanilloidreceptorsubtype1(VR1),andVR1-likeimmunoreactivity,inthecentralnervoussystemoftheratandhuman.[J].ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,2000,97(7).:365-3660
[23]SekiN,ShirasakiH,KikuchiM ,etal.ExpressionandlocalizationofTRPV1inhumannasalmucosa[J].Rhinology,2006,44(2):128-134.
[24]DinhQT,GronebergDA,MingomatajE,etal.ExpressionofsubstancePandvanilloidreceptor(VR1)intrigeminalsensoryneuronsprojectingtothemousenasalmucosa[J].Neuropeptides,2003,37(4):245-250.
[25]HamkeM,HerpferI,LiebK,etal.SubstancePinducesexpressionofthecorticotropin-releasingfactorreceptor1byactivationoftheneurokinin-1receptor[J].BrainResearch,2006,1102(1):135-144.

备注/Memo

备注/Memo:
基金项目：南京市卫生科技发展专项资金资助项目（ＹＫＫ１７１９２、ＹＫＫ１８１７２）
更新日期/Last Update: 2021-06-08