[1]李磊 吴东良 阎玥 史琦 司东旭 李友林.基于网络药理学探讨徐长卿-紫草-地肤子 治疗湿疹的作用机制[J].现代中医药,2022,1(01):040-47.[doi:10.13424/j.cnki.mtcm.2022.01.007]
　LI Lei WU Dongliang YAN Yue SHI Qi SI Dongxu LI Youlin.Study on Mechanism of Xuchangqing-Zicao-Difuzi in Treatment of Eczema Based on Network Pharmacology[J].Modern Traditional Chinese Medicine,2022,1(01):040-47.[doi:10.13424/j.cnki.mtcm.2022.01.007]
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基于网络药理学探讨徐长卿-紫草-地肤子 治疗湿疹的作用机制
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
1
期数:
2022年01期
页码:
040-47
栏目:
方药纵横
出版日期:
2022-01-20

文章信息/Info

Title:
Study on Mechanism of Xuchangqing-Zicao-Difuzi in Treatment of Eczema Based on Network Pharmacology
文章编号:
1672-0571(2022)01-0040-08
作者:
李磊1; 2 吴东良1; 2 阎玥2 史琦2 司东旭1; 2 李友林2
1.北京中医药大学，北京 100029；
2.中日友好医院国家中医药管理局重点研究室, 中医药防治过敏性疾病北京市重点实验室，北京 100029
Author(s):
LI Lei1; 2 WU Dongliang1; 2 YAN Yue2 SHI Qi2 SI Dongxu11; 2 LI Youlin2
1.Beijing University of Traditional Chinese Medicine, Beijing 100029,China;
2.China Japan Friendship Hospital, Key Research Office of the State Administration of Traditional Chinese Medicine,Beijing Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Allergic Diseases,Beijing 100029,China
关键词:
关键词：湿疹; 徐长卿; 紫草; 地肤子; 网络药理学; 信号通路
Keywords:
Key words：Eczema;  Xuchangqing;  Zicao;  Difuzi;  Network pharmacology;  Signal pathway
分类号:
R275.9
DOI:
10.13424/j.cnki.mtcm.2022.01.007
文献标志码:
A
摘要:
摘 要：目的 通过网络药理学探讨徐长卿-紫草-地肤子治疗湿疹的有效成分及作用机制。方法 首先，利用TCMSP平台检索徐长卿、紫草、地肤子的化学成分和作用靶点，在DrugBank、OMIM、GeneCards和TTD四个数据库筛选出治疗湿疹的靶标；其次，通过Uniprot数据库对靶点进行基因注释，利用Venny 2.1在线工具获取药物-疾病交集基因；再者，在Cytoscape3.7.1软件上构建徐长卿-紫草-地肤子与湿疹的疾病靶点网络，再用STRING构建PPI蛋白相互作用网络；最后，通过DAVID进行KEGG通路富集分析和GO功能富集分析。结果 徐长卿、紫草、地肤子共筛选出白薇苷B、乙酰紫草素、11,14二十碳二烯酸甲酯等20个有效成分及PTGS2、ADRB2等49个靶点，在疾病库中共检索到湿疹的有效靶点1702个，取二者交集获得19个交集靶点。通过GO功能富集分析得到24个GO条目(P＜0.05)，KEGG富集分析得到10条信号通路(P＜0.05)，主要涉及花生四烯酸代谢等信号途径。结论 初步展示了徐长卿-紫草-地肤子可能通过花生四烯酸代谢等信号通路改善湿疹的作用机制，以期对临床及相关实验研究提供一定参考。
Abstract:
Abstract：Objective To explore the effective components and mechanism of Xuchangqing-Zicao-Difuzi in the treatment of eczema through network pharmacology. Methods Firstly, TCMSP platform was used to search the chemical components and action targets of Cynanchum paniculatum, Arnebia and Fructus rehmanniae, and the targets for the treatment of eczema were screened in four databases:DrugBank,OMIM,GeneCards and TTD; Secondly, the target gene was annotated through Uniprot database, and the drug disease intersection gene was obtained by Venny 2.1 online tool; Moreover, in Cytoscape 3 7.1 construct the disease target network of Xuchangqing-Zicao-Difuzi and eczema on the software, and then construct the PPI protein interaction network with string; Finally, KEGG pathway enrichment analysis and go function enrichment analysis were carried out by David. Results A total of 20 active components such as baicalin B, acetylshikonin, 11,14 eicosadienoic acid methyl ester and 49 targets such as PTGS2 and ADRB2 were screened by Xuchangqing,Zicao and Difuzi. A total of 1702 effective targets of eczema were retrieved in the disease library, and 19 intersection targets were obtained. 24 GO items were obtained by GO functional enrichment analysis (P<0.05), and 10 signal pathways were obtained by KEGG enrichment analysis (P<0.05), mainly involving arachidonic acid metabolism and other signal pathways. Conclusion It is preliminarily demonstrated that the action mechanism of Xuchangqing-Zicao-Difuzi may improve eczema through signal pathways such as arachidonic acid metabolism, so as to provide some reference for clinical and related experimental research.

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备注/Memo

备注/Memo:
基金项目：中医药行业科研专项（2015468001）
更新日期/Last Update: 2022-02-18