[1]陈紫莹 黄姣丽 廖映红 陈佳婷 孙旭高 陈星 肖遂.复方铁苋工艺优化及抑菌成分研究[J].现代中医药,2024,(04):100-108.[doi:10.13424/j.cnki.mtcm.2024.04.019]
　CHEN Ziying HUANG Jiaoli LIAO Yinghong CHEN JiatingSUN Xugao CHEN Xing XIAO Sui.Optimization of Fufang Tiexian Process andStudy on Antibacterial Components[J].Modern Traditional Chinese Medicine,2024,(04):100-108.[doi:10.13424/j.cnki.mtcm.2024.04.019]
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复方铁苋工艺优化及抑菌成分研究()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年04期
页码:
100-108
栏目:
出版日期:
2024-08-28

文章信息/Info

Title:
Optimization of Fufang Tiexian Process andStudy on Antibacterial Components
文章编号:
1672-0571(2024)04-0100-09
作者:
陈紫莹 黄姣丽 廖映红 陈佳婷 孙旭高 陈星 肖遂
华南农业大学林学与风景园林学院，广东 广州 510642
Author(s):
CHEN Ziying HUANG Jiaoli LIAO Yinghong CHEN JiatingSUN Xugao CHEN Xing XIAO Sui
School of Forestry and Landscape Architecture,South China Agricultural University, Guangzhou 510642, China
关键词:
关键词：铁苋菜; 五倍子; 诃子; UPLC-Q-TOF-MS; 嗜水气单胞菌; 工艺优化; 谱效关系
Keywords:
Key words:Acalypha australis Linn.; gallnut; Terminalia chebula Retz.;  UPLC-Q-TOF-MS;  Aeromonas hydrophila;  Process optimization;  Spectral effect relationship
分类号:
R284.1
DOI:
10.13424/j.cnki.mtcm.2024.04.019
文献标志码:
A
摘要:
摘 要：目的 基于抑菌药效优化复方铁苋最佳配伍及提取工艺，并揭示该中药复方的抑菌物质。方法 以抗嗜水气单胞菌药效为考察指标筛选复方铁苋最佳配伍，以没食子酸的含量和抑菌活性为评价指标，通过单因素实验和响应面法优化复方铁苋的提取工艺；对不同提取工艺的复方铁苋建立化学指纹图谱并指认“共有峰”，通过相关分析建立指纹图谱与抑菌药效间的“谱效关系”，并采用UPLC-Q-TOF-MS技术进行成分鉴定，找出抑制嗜水气单胞菌的关键成分。结果 复方铁苋以铁苋菜∶五倍子∶诃子=2∶2∶1（w/w/w）为最佳配伍，以料液比为1∶25，浸泡22 min后煎煮60 min为最佳提取工艺，该条件下复方铁苋提取物对嗜水气单胞菌具有最佳抑制效果；从复方铁苋指纹图谱中找出4个与抑菌活性呈高度正相关的物质：诃子酸＞诃子鞣酸＞没食子酸-4-O-（6-O-没食子酰）葡萄糖苷＞没食子酸（相关系数在0.499~0.846之间）。结论 铁苋菜、五倍子、诃子在最佳配伍及工艺条件下具有明显抑制嗜水气单胞菌的活性，通过“谱效关系”快速找出该中药复方中潜在的抑菌物质，为复方铁苋进一步开发为防治嗜水气单胞菌的药物提供科学依据。
Abstract:
Abstract：Objective To optimize the optimal combination and extraction process of Fufang Tiexian based on its antibacterial efficacy, and to reveal the antibacterial substances of this traditional Chinese medicine compound.Methods The optimal combination of Fufang Tiexian was selected based on the efficacy of anti Pseudomonas aeruginosa as the evaluation index, and the content of gallic acid and antibacterial activity were used as the evaluation index. The extraction process of Fufang Tiexian was optimized through single factor experiments and response surface methodology; Establish chemical fingerprints of Fufang Tiexian with different extraction processes and identify “common peaks”. Through correlation analysis, establish a “spectrum effect relationship” between the fingerprint and antibacterial efficacy, and use UPLC-Q-TOF-MS technology for component identification to identify the key components that inhibit Aeromonas hydrophila. Results The optimal combination of Fufang Tiexian was found to be Acalypha australis Linn.∶gallnut∶Terminalia chebula Retz.=2∶2∶1 (w/w/w), with a solid-liquid ratio of 1∶25. The optimal extraction process was to soak for 22 minutes and then boil for 60 minutes. Under these conditions, the extract of Fufang Tiexian had the best inhibitory effect on Aeromonas hydrophila; Four substances highly positively correlated with antibacterial activity were identified from the fingerprint of Fufang Tiexian∶chebulic acid＞chebulic acid＞gallic acid 4-O-(6-O-gallic acid) glucoside＞gallic acid (correlation coefficient between 0.499 and 0.846). Conclusion Acalypha australis Linn, Chinese gallnut, and Terminalia have significant inhibitory activity against Pseudomonas aeruginosa under the optimal combination and process conditions. Through the “spectrum effect relationship”, potential antibacterial substances in this traditional Chinese medicine compound can be quickly identified, providing scientific basis for the further development of Fufang Tiexian as a drug for the prevention and treatment of Pseudomonas aeruginosa.

参考文献/References:

［1］DOUKAS,ATHANASSOPOULOU,KARAGOUNI,et al.Short communication Aeromonas hydrophila infection in cultured sea bass,Dicentrarchus labrax L.,and Puntazzo puntazzo Cuvier from the Aegean Sea［J］.Journal of Fish Diseases,1998,21(4):317-320.
［2］KOZISKA A,PKALA A.Characteristics of disease spectrum in relation to species,serogroups,and adhesion ability of motile aeromonads in fish［J］.The Scientific World Journal,2012,2012:949358.
［3］SHARPLESS M,SHARMA R.Aeromonas hydrophila endogenous endophthalmitis in a patient with underlying adenocarcinoma of the colon［J］.BMJ Case Reports,2021,14(4):e241317.
［4］KHALIL W,GANTOIS C,Lemnos L,et al.Aeromonas hydrophila is a deceptive pathogen requiring reconsideration of antibiotic prophylaxis［J］.Surgical Infections,2021,22(9):987-988.
［5］江丽莉,朱延焱,余璟璐,等.2例外伤感染嗜水气单胞菌致坏死性筋膜炎的病例特点及临床转归［J］.浙江医学教育,2018,17(5):55-57.
［6］NHINH DT,LE DV,VAN VAN K,et al.Prevalence,virulence gene distribution and alarming the multidrug resistance of Aeromonas hydrophila associated with disease outbreaks in freshwater aquaculture［J］.Antibiotics,2021,10(5):532.
［7］HOSSAIN S,DE SILVA BCJ,DAHANAYAKE PS,et al.Characterization of virulence properties and multi‐drug resistance profiles in motile Aeromonas spp.isolated from zebrafish (Danio rerio)［J］.Letters in Applied Microbiology,2018,67(6):598-605.
［8］刘华钢,申庆荣,刘丽敏.中药抗菌研究进展［J］.时珍国医国药,2010,21(2):463-465.
［9］梁曾恩妮.铁苋菜主要功能成分及其体外抑菌研究［D］.长沙:湖南农业大学,2008.
［10］杨香玉,李思迪,麻成金,等.五倍子及其复方对痤疮致病细菌的抑制作用和配伍配比研究［J］.中华中医药杂志,2021,36(1):467-472.
［11］王涛,陈家顺,姚康,等.诃子及其提取物研究进展及畜牧业应用潜力展望［J］.饲料工业,2023,44(22):29-33.
［12］孟祥锋.广谱抑菌中草药诃子的抑菌活性分析及有效组分的初步分离检测［D］.大连:辽宁师范大学,2009.
［13］王帅兵,张雅城.5种中药提取物及其组合对嗜水气单胞菌的体外抑制作用［J］.饲料研究,2023,46(22):80-85.
［14］肖遂.基于谱效关系的中药铁苋菜抑菌物质辨识方法研究［D］.北京:中国农业科学院,2013.
［15］彭金菊,马驿,梁淑鋆,等.抗菌中药及复方对嗜水气单胞菌的体外抑制效果［J］.安徽农业科学,2009,37(28):3623-3625.
［16］夏国文,杜小东,胡庆丰.没食子酸对嗜水气单胞菌及温和气单胞菌的体外抑菌作用研究［J］.辽宁中医杂志,2015,42(5):1127-1129.
［17］张晓莉,刘宇灵,樊丽姣,等.基于体外抗氧化活性的参芪通脉益智方提取工艺评价及其谱效关系的初步研究［J］.中国中药杂志,2016,41(9):1635-1639.
［18］刘丁丽.胡颓子多酚的提取鉴定及抗氧化活性研究［D］.长沙:中南林业科技大学,2021.
［19］崔小敏,万兆新,任慧,等.基于UPLC-Q-Orbitrap HRMS技术的白毛银露梅化学成分研究［J］.中药材,2020,43(8):1901-1906.
［20］阚玉红,谢笔钧,孙智达.胭脂红番石榴叶黄酮提取物的抑菌活性及其机理［J］.中国调味品,2021,46(12):159-166,188.
［21］樊晓荃,付娟,胡军华,等.UPLC-Q-TOF-MS/MS快速分析六味地黄苷糖片化学成分［J］.中草药,2021,52(21):6473-6484.
［22］孙国东,霍金海,王改丽,等.基于UPLC-Q-TOF/MS技术的核桃楸皮成分分析［J］.中草药,2017,48(4):657-667.
［23］韩贤威.不同产地西青果与诃子成分含量的比较及抗细菌毒力物质研究［D］.大连:辽宁师范大学,2019.
［24］李文兰,孙志,程斌,等.金匮肾气丸的紫外光谱-质谱/质谱研究［J］.光谱学与光谱分析,2008,28(8):1914-1918.
［25］林启凰,鲍红娟,张岗.UPLC-MS/MS法分析通奶草乙酸乙酯部位成分及其体外抗菌活性［J］.中成药,2020,42(7):1931-1935.
［26］唐明敏,田恒康,杨文宁,等.桑叶水提物及其含药血清化学成分分析［J］.中国实验方剂学杂志,2016,22(9):25-29.
［27］程鼎.翻白草多酚的分离鉴定及生物活性研究［D］.南昌:南昌大学,2021.
［28］姜廷良,霍海如.重视中药多组分整合作用的研究［J］.世界科学技术,2003,5(2):1-4,75.
［29］肖佳妹,杨岩,周晋,等.基于总量统计矩理论的中药质量标志物研究策略［J］.中草药,2019,50(19):4589-4594.
［30］BORGES A,FERREIRA C,SAAVEDRA MJ,et al.Antibacterial activity and mode of action of ferulic and Gallic acids against pathogenic bacteria［J］.Microbial Drug Resistance,2013,19(4):256-265.
［31］向丽,周铁军,叶迎春,等.诃子鞣质对白色念珠菌及其生物被膜的影响［J］.重庆医学,2013,42(2):134-137.
［32］周梦宇,周本宏,郭咸希.石榴皮鞣质作为天然抑菌剂的研究进展［J］.中国药师,2018,21(11):2037-2040,2044.
［33］彭齐,谢丽玲,谢俊.中药抑菌机制的研究方法［J］.生物技术进展,2015,5(1):66-69.
［34］余苏云,陆茵.中药起效作用机制探讨［J］.中国药理学与毒理学杂志,2018,32(5):347-354.
［35］李娟,张学顺,傅春升.中药抗菌作用的研究进展［J］.中国药业,2014,23(2):90-93.

备注/Memo

备注/Memo:
基金项目：广东省自然科学基金面上项目（2021A1515012153）
更新日期/Last Update: 2024-07-15