[1]赵秋芳 汪雪梅 李强 张唯.基于PI3K-AKT信号通路探讨蒺藜皂苷对Graves眼病细胞凋亡的影响*[J].现代中医药,2024,(06):096-101.[doi:10.13424/j.cnki.mtcm.2024.06.017]
　ZHAO Qiufang WANG Xuemei LI Qiang ZHANG Wei.Exploration of the Effect of Saponins from Tribulus Terrestrison Apoptosis of Graves’ Ophthalmopathy Cells Basedon the PI3K-AKT Signaling Pathway[J].Modern Traditional Chinese Medicine,2024,(06):096-101.[doi:10.13424/j.cnki.mtcm.2024.06.017]
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基于PI3K-AKT信号通路探讨蒺藜皂苷对Graves眼病细胞凋亡的影响*()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年06期
页码:
096-101
栏目:
出版日期:
2024-11-20

文章信息/Info

Title:
Exploration of the Effect of Saponins from Tribulus Terrestrison Apoptosis of Graves’ Ophthalmopathy Cells Basedon the PI3K-AKT Signaling Pathway
文章编号:
1672-0571(2024)04-0087-10
作者:
赵秋芳 汪雪梅 李强 张唯
陕西中医药大学附属医院眼科，陕西 咸阳 712000
Author(s):
ZHAO Qiufang WANG Xuemei LI Qiang ZHANG Wei
Ophthalmology Department, Affiliated Hospital of Shaanxi University of Chinese Medicine, Shaanxi Xianyang 712000,China
关键词:
关键词：蒺藜皂苷; PI3K; AKT; Graves 眼病; Nthy-ori-3-1
Keywords:
Key words:Saponins from Tribulus terrestris;  PI3K;  AKT;  Graves’ ophthalmopathy;  Nthy-ori-3-1
分类号:
R581.9
DOI:
10.13424/j.cnki.mtcm.2024.06.017
文献标志码:
A
摘要:
摘 要：目的 探究蒺藜皂苷对Graves病体外细胞模型人甲状腺正常细胞Nthy-ori-3-1凋亡的影响及其分子机制。方法 M22 诱导人甲状腺滤泡上皮细胞株（Nthy-ori-3-1）建立Graves体外细胞模型，设对照组、模型组、蒺藜皂苷处理组（5、10、20 ng·mL-1）。CCK-8评估各组Nthy-ori-3-1细胞增殖能力；ELISA评估各组细胞培养液中cAMP变化；TUNEL评估各组细胞凋亡情况；免疫荧光评估各组细胞Bcl-2、Bax和Caspase-3变化；Western Blot评估PI3K、AKT、p-PI3K、p-AKT蛋白变化。结果 高浓度蒺藜皂苷促进Nthy-ori-3-1细胞凋亡，抑制Nthy-ori-3-1细胞增殖；高浓度蒺藜皂苷抑制M22 诱导Nthy-ori-3-1细胞cAMP表达；高浓度蒺藜皂苷促进Bax和Caspase-3表达，抑制Bcl-2表达；高浓度蒺藜皂苷抑制p-PI3K和p-AKT表达。结论 高浓度蒺藜皂苷抑制PI3K-AKT信号通路促进Graves细胞凋亡。
Abstract:
Abstract：Objective To investigate the effect and molecular mechanism of saponins from Tribulus terrestris on apoptosis of human thyroid normal cells Nthy-ori-3-1 in an in vitro cell model of Graves’ disease. Methods M22 induced human thyroid follicular epithelial cell line (Nthy-ori-3-1) to establish an in vitro Graves cell model, with control group, model group, and Tribulus terrestris saponin treatment group (5, 10, 20 ng·mL-1). CCK-8 evaluation of the proliferation ability of Nthy-ori-3-1 cells in each group; ELISA evaluation of cAMP changes in cell culture media of each group; TUNEL evaluation of apoptosis in each group of cells; Immunofluorescence evaluation of changes in Bcl-2, Bax, and Caspase-3 in each group of cells; Western Blot was used to evaluate changes in PI3K, AKT, p-PI3K, and p-AKT proteins. Results The high concentration of saponins from Tribulus terrestris promotes apoptosis of Nthy-ori-3-1 cells and inhibits their proliferation; High concentration saponins from Tribulus terrestris inhibit cAMP expression induced by M22 in Nthy-ori-3-1 cells; High concentration saponins from Tribulus terrestris promote the expression of Bax and Caspase-3, while inhibiting the expression of Bcl-2; High concentration saponins from Tribulus terrestris inhibit the expression of p-PI3K and p-AKT. Conclusion High concentration saponins from Tribulus terrestris inhibit the PI3K-AKT signaling pathway and promote apoptosis in Graves’ cells.

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备注/Memo

备注/Memo:
基金项目：陕西省重点研发计划项目（2017SF-350）
更新日期/Last Update: 2024-11-21