[1]岳 明,马　丽,仝　敏,等.ＨＰＬＣ法测定花仙宫血胶囊中人参皂苷Ｒｇ１的含量[J].现代中医药,2019,(03):095-98,119.[doi:10.13424/j.cnki.mtcm.2019.03.030]
　Yue Ming Ma Li Quan Min Zhang Enhu.Determination of Ginsenoside Rg1 in Huaxian Gongxue Capsules by HPLC[J].Modern Traditional Chinese Medicine,2019,(03):095-98,119.[doi:10.13424/j.cnki.mtcm.2019.03.030]
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ＨＰＬＣ法测定花仙宫血胶囊中人参皂苷Ｒｇ１的含量()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
095-98,119
栏目:
方药研究
出版日期:
2019-05-30

文章信息/Info

Title:
Determination of Ginsenoside Rg1 in Huaxian Gongxue Capsules by HPLC
文章编号:
1672-0571(2019)03-0095-05
作者:
岳 明; 马　丽; 仝　敏; 张恩户
1.咸阳市中心医院,陕西咸阳712000;2.陕西中医药大学,陕西咸阳712046
Author(s):
Yue Ming 1 Ma Li 1 Quan Min 1 Zhang Enhu 2
1. Xianyang Central Hospital, Xianyang China, 712000; 2. Shaanxi University of Chinese Medicine, Xianyang China, 712046
关键词:
花仙宫血胶囊; 人参皂苷Rg1; 高效液相色谱
Keywords:
Huaxian Gongxue Capsules;  ginsenoside Rg1;  High Performance Liquid Chromatography( HPLC)
分类号:
R284
DOI:
10.13424/j.cnki.mtcm.2019.03.030
文献标志码:
A
摘要:
目的测定花仙宫血胶囊中人参皂苷Rg1的含量,建立花仙宫血胶囊质量控制的含量测定标准?方法采用高效液相色谱法(HPLC),色谱柱:Kromosil ODS C18(5μm,200×4.6mm);流动相:乙腈-0.05%磷酸溶液(24:76);检测波长:203nm;流速:1.0mL/min?结果人参皂苷Rg1在0.85-8.50μg线性范围间呈良好线性关系(r=0.9994,n=6);平均加样回收率为105.80%(RSD=0.74%,n=6)?结论HPLC法测定花仙宫血胶囊中人参皂苷Rg1方法准确?操作简便
Abstract:
To determine the content of ginsenoside Rg1 in Huaxian Gongxue Capsules and establish the quality control standard for Huaxian Gongxue Capsules. Methods: high performance liquid chromatography ( HPLC) was adopted, chromatographic column: Kromosil ODS C18 ( 5 micron, 200 x 4. 6 mm) ; mobile phase: acetonitrile -0. 05% phosphoric acid solution ( 24: 76) ; detection wavelength: 203 nm; flow rate: 1. 0 ml/ min. Results: There was a good linear relationship for ginsenoside Rg1 ranging 0. 85 - 8. 50 UG ( r = 0. 9994, n = 6) , and the average recovery was 105. 80% ( RSD = 0. 74%, n = 6) . Conclusion: Determination of ginsenoside Rg1 in Huaxian Gongxue Capsules by HPLC is accurate, simple, stable and reliable. It can be used for the content control of Huaxian Gongxue Capsules.

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更新日期/Last Update: 2019-05-15