[1]占永久１,孟一峰１,陈佳２,等.加味六味地黄汤对人毛乳头细胞增殖及 ＶＥＧＦ表达的影响[J].现代中医药,2019,(05):098-102.[doi:10.13424/j.cnki.mtcm.2019.05.030]
　Zhan Yongjiu Meng Yifeng Chen Jia Yan Xuemei Xu Feng Zhu Xiaojing.Effect of Modified Liuwei Dihuang Decoction on the Proliferation of Human Dermal Papilla Cells and the Expression of Vascular Endothelial Growth Factor[J].Modern Traditional Chinese Medicine,2019,(05):098-102.[doi:10.13424/j.cnki.mtcm.2019.05.030]
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加味六味地黄汤对人毛乳头细胞增殖及 ＶＥＧＦ表达的影响()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
098-102
栏目:
方药研究
出版日期:
2019-09-16

文章信息/Info

Title:
Effect of Modified Liuwei Dihuang Decoction on the Proliferation of Human Dermal Papilla Cells and the Expression of Vascular Endothelial Growth Factor
文章编号:
1672-0571(2019)05-0098-05
作者:
占永久１; 孟一峰１; 陈佳２; 严雪妹１; 徐丰１; 朱晓静１
苏州市中西医结合医院
Author(s):
Zhan Yongjiu 1 Meng Yifeng 1 Chen Jia 2 Yan Xuemei 1 Xu Feng 1 Zhu Xiaojing 1
1. Suzhou TCM and Western Hospital, Suzhou China, 215000; 2. Shanghai Dermatology Hospital, Shanghai China, 200443
关键词:
加味六味地黄汤; 毛乳头细胞; 细胞增殖; ＶＥＧＦ
Keywords:
Modified Liuwei Dihuang Decoction;  dermal papilla cells;  cell proliferation;  VEGF
分类号:
R285.6
DOI:
10.13424/j.cnki.mtcm.2019.05.030
文献标志码:
A
摘要:
观察加味六味地黄汤对人毛乳头细胞增殖和分泌血管内皮生长因子（ＶＥＧＦ）的影响。方法 分别采用低、中、高浓度的含加味六味地黄汤大鼠血清作用于体外培养的人毛乳头细胞，用四甲基偶氮唑蓝 （ＭＴＴ）法测定细胞增殖活性；ＥＬＩＳＡ法检测细胞培养上清液中 ＶＥＧＦ蛋白含量。结果 与对照组相比，加味六味 地黄汤对细胞增殖的影响不明显，无统计学意义（Ｐ! ０．０５）；在分泌 ＶＥＧＦ方面，中、高浓度的加味六味地黄汤 组促分泌作用明显，且具有量效关系，差异有统计学意义（Ｐ" ０．０５，Ｐ" ０．０１）。结论 加味六味地黄汤对人毛 乳头细胞增殖无显著影响，但能明显促进毛乳头细胞 ＶＥＧＦ的分泌，加味六味地黄汤可能是通过增加毛乳头细 胞 ＶＥＧＦ的分泌来促进毛发的生长
Abstract:
To observe the effect of Modified Liuwei Dihuang Decoction on the proliferation and secretion of vascular endothelial growth factor ( VEGF) in human dermal papilla cells. Methods: Human dermal papilla cells cultured in vitro were treated with rat serum containing Modified Liuwei Dihuang Decoction at low, medium and high concentrations. Cell proliferation activity was measured by MTT method, and the content of VEGF protein in cell culture supernatant was detected by ELISA method. Results: Compared with the control group, the effect of Modified Liuwei Dihuang Decoction on cell proliferation was not significant ( P > 0. 05) ; in the secretion of vascular endothelial growth factor, the medium and high concentration of Modified Liuwei Dihuang Decoction group had obvious secretion promoting effect, and had dose - effect relationship, the difference was statistically significant ( P < 0. 05, P < 0. 01) . Conclusion: Modified Liuwei Dihuang Decoction has no significant effect on the proliferation of human dermal papilla cells, but it can significantly promote the secretion of VEGF in human dermal papilla cells. Modified Liuwei Dihuang Decoction may promote hair growth by increasing the secretion of VEGF in dermal papilla cells.

参考文献/References:

[1]王纪云.六味地黄丸治愈脱发1例[J].山西中医,1999,15(1):24.
[2]张理保.六味地黄丸治疗斑秃22例[J].湖南中医杂志,2001,17(4):44.
[3]曹玉杰.中西医结合治疗斑秃疗效观察[J].中国民间疗法,2014,22(10):64.
[4]占永久,詹业顺,张晓杰.“止血”治脱发———“发为血之余”之理论发挥[J].中华中医药杂志,2014,29(5):1524-1527.
[5]何天林.重用补骨脂治疗脱发1例[J].中国社区医师,2007,9(166):58.
[6]占永久.旱莲草“乌须发”作用浅析[J].中医药导报,2015,8:84-85.
[7] Xiong Y, Liu Y, Song Z, et al. ldentification of Wnt/ beta- catenin signaling pathway in dermal papilla cells of human scalp hair follicles: TCF4 regulates the proliferation and secretory activity of dermal papilla cell [ J] . J Dermatol,2014,41 ( 1) :84 -91.
[8]林恩,祝宁侠,蔡博治,等.LncRNAs和miRNAs在毛乳头细胞衰老与毛囊再生中的作用[J].中国美容整形外科杂志,2018,29(7):435-437.
[9]王磊,范卫新.毛囊生物学基础知识[J]中国医学文摘?皮肤科学,2016,33(4):409-414.
[10]李维珊,柯昌能,齐德丽.EGF?bFGF对促进人毛乳头细胞生长及分化的影响[J].临床医学工程,2014.2(21):139-140.
[11]朱海琴,范卫新,张卉.甘草提取物对人毛乳头细胞增殖和分泌血管内皮生长因子的影响[J].临床皮肤科杂志,2007,36(4):201-203.
[12] Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF mediated angiogenesis[ J] . J Clin Invest,2001,107( 4) :409 -417.
[13]范卫新,朱文元.55种中药对小鼠触须毛囊体外培养生物学特性的研究[J]临床皮肤科杂志,2001,30(2):81-84.
[14]张兴洪,范卫新.何首乌?女贞子等中药煎剂对体外培养的猪毛囊毛发生长的影响[J].中华皮肤科杂志,2005,38(2):102-104.
[15]孙宇,杨淑霞,涂平,等.女贞子等对人头皮毛囊体外培养影响的研究[J].中华皮肤科杂志,2005.38(5):303-305.
[16]吴巧云.红花?当归和生侧柏叶煎剂对鼠毛囊生长影响的体外研究[D].杭州:浙江大学,2006.
[17]李东海,陈罗娣,齐庆,等.补发1号方促毛乳头细胞增殖及其分子机制的实验研究[J].中国现代医学杂志,2012,22(31):25-29.
[18]何红梅,朱红霞,刘强,等.何首乌提取物对C57BL/6J小鼠毛囊生长和毛发生长周期的影响[J].中国实验方剂学杂志,2012,18(23):216-219.
[19]徐莲,吴岚,王雪,等.黄芪?何首乌?女贞子?菟丝子混合提取物对体外培养毛囊生长的影响及药理作用研究[J].现代生物医学进展,2014,22(8):4201-4204.
[20]王任,袁婷,周雯,等.桑柏生发方对毛发再生过程中VEGF等细胞因子的影响[J].中医药导报,2016,22(15):24-27.
[21]万力生,范红霞.中医治疗脱发的方药统计与述评[J].浙江中西医结合杂志,2000,10(9):564-565.
[22]沈璐,陈科力.中医药治疗脱发的研究与分析[J].中南民族大学学报(自然科学版),2011,30(1):42-45.
[23]何天林.重用补骨脂治疗脱发1例[J].中国社区医师,2007,9(166):58.

备注/Memo

备注/Memo:
基金项目：苏州市科技发展计划项目（ＳＹＳＤ２０１５１５６）
更新日期/Last Update: 2019-09-15