[1]韦庄怡,任小朋,张泳,等.清腹通肠冲剂对急性肺损伤大鼠TNF-α?NF-κBp65的影响[J].现代中医药,2020,(06):020-24,41.[doi:10.13424/j.cnki.mtcm.2020.06.005]
 Wei Zhuangyi,Ren Xiaopeng,Zhang Yong,et al.Effect of Qingfu Tongchang Granule on TNF - α and NF – κB p65 in ALI Rats[J].Modern Traditional Chinese Medicine,2020,(06):020-24,41.[doi:10.13424/j.cnki.mtcm.2020.06.005]
点击复制

清腹通肠冲剂对急性肺损伤大鼠TNF-α?NF-κBp65的影响()
分享到:

《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
020-24,41
栏目:
出版日期:
2023-11-20

文章信息/Info

Title:
Effect of Qingfu Tongchang Granule on TNF - α and NF – κB p65 in ALI Rats
文章编号:
1672-0571(2020)06-0020-06
作者:
韦庄怡任小朋张泳高山邢明亮
陕西中医药大学附属医院,陕西咸阳712000
Author(s):
Wei Zhuangyi Ren Xiaopeng Zhang Yong Gao Shan Xing Mingliang
The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang Shaanxi, 712000
关键词:
清腹通肠冲剂TNF-αNF-κBp65脓毒症急性肺损伤
Keywords:
Qingfu Tongchang Granules TNF - α NF - κBp65 pyemia ALI
分类号:
R256.1
DOI:
10.13424/j.cnki.mtcm.2020.06.005
文献标志码:
A
摘要:
目的观察清腹通肠冲剂对脓毒症大鼠急性肺损伤(ALI)的作用?方法通过40只大鼠建立尾静脉注射LPS建立脓毒症大鼠模型,随机分为4组(n=10):空白组?模型组?地塞米松对照组?清腹通肠组?各组分别于造模2日后检测肺组织湿干重比(W/D)?通过HE染色法光镜下观察肺组织病理变化;ELISA法检测血清中TNF-α含量?Western-blot法测量肺组织中NF-κBp65表达情况?结果清腹通肠组血清中TNF-α水平明显低于模型组(P<0.05)?清腹通肠组肺组织W/D?NF-κBp65表达量及肺组织损伤评分均明显低于模型组(P<0.05)?结论清腹通肠冲剂可以通过减轻肺组织炎症反应程度,有效保护肺组织损伤?
Abstract:
Objective To observe the effect of Qingfu Tongchang( clearing lung and dredging intestine) Granule on ALI ( acute lung injury) in rats with pyemia. Methods A pyemia rat model was established by injecting LPS into the tail vein of 40 rats, and then they were randomly divided into 4 groups ( n =10) : blank group, model group, dexamethasone control group, and Qingfu Tongchang group. 2 days after modeling, the pathological changes of lung tissue were observed under light microscope by HE staining method and serum TNF - α content was detected by ELISA method. Western – blot method was used to measure the expression of NF - κB p65 in lung tissue. Results The serum TNF - α level in the Qingfu Tongchang group was significantly lower than that of the model group ( P <0. 05) . The lung tissue W/ D, NF - κB p65 expression and lung tissue injury scores of the Qingfu Tongchang group were significantly lower than those of the model group ( P <0. 05) . Conclusion Qingfu Tongchang Granule can effectively protect lung tissue damage by reducing the degree of lung inflammation.

参考文献/References:

[1]中国严重脓毒症/脓毒性休克治疗指南(2014)[J].全科医学临床与教育,2015,13(4):365-367.
[2] Protective effect and mechanism of recessin D1 on acute lung injury in sepsis [D] SHANDONG UNIVERSITY,2017.
[3] Shi H, Ren CS. Basis and clinical research of acute lung injury/acute respiratory distress syndrome, Chin J Lung Dis(Electronic Edition), August 2013,6(4):350-355.
[4]李旭,陈岩,白雪松,等.中药治疗急性肺损伤/急性呼吸窘迫综合征作用机制研究进展[J].辽宁中医药大学学报,2017,19(1):166-170.
[5]葛金林,余雯文,曾余丰,等.汉黄芩素对急性肺损伤小鼠的保护作用[J].温州医科大学学报,2019,49(1):38-42.
[6]郭丽纳,孙燕妮.大黄素对急性肺损伤保护作用研究进展[J].中国中医急症,2017,26(4):663-665.
[7]程晓平.基于NRF2/ARE信号通路探讨红藤水提物对炎症反应的调控作用[D].福州:福建中医药大学,2017.
[8]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1069-1072.
[9] Murakami K, McGuire R, Cox RA, et al. Heparin nebulization attenuates acute lung injury in Sepsis following smoke inhalation in sheep[ J] . Shock,2002,18( 3) :236 -241.
[10] Mrozek J D, Smith K M, Bing D R, et al. Exogenous surfactant and partial liquid ventilation: physiologic and pathologic effects,1997,156(41) :1058-65.
[11]魏婧婧,杨秀惠.古代医家对热喘的辨证施治概述[J].河北中医,2008,30(6):665-666.
[12]庄先飞,俞苏岚,樊梦霖,等.中药复方防治急性肺损伤的作用机制研究进展[J].中国实验方剂学杂志,2012,18(17):326-331.
[13]乐海浪,罗国强.创伤后早期炎症因子TNF-a?IL-1?IL-6的研究进展[J].现代诊断与治疗,2014,25(4):763-765.
[14]林丹丹,刘海燕.白细胞介素-1与肿瘤[J].免疫学杂志,2010,26(12):1099-1103,1106.
[15]郭隽,张立实,彭双清.NF-κB在MT对LPS致肝脏炎症损伤保护效应中的作用[J].毒理学杂志,2014,28(5):364-369.
[16]王晓晨,吉爱国.NF-κB信号通路与炎症反应[J].生理科学进展,2014,45(1):68-71.

相似文献/References:

[1]郭建平 夏勤 顿文亮 吴?岚 黄书亮/sup>.槐花散治疗溃疡性结肠炎肠风下血证作用机制研究[J].现代中医药,2020,(05):009.[doi:10.13424/j.cnki.mtcm.2020.05.003]
 Guo Jianping,Xia Qin,Dun Wenliang,et al.A Study on the Effect and Mechanism of Huaihua Powder in the Treatment of Ulcerative Colitis by Emulating the Intestinal Subwind Blood Syndrome[J].Modern Traditional Chinese Medicine,2020,(06):009.[doi:10.13424/j.cnki.mtcm.2020.05.003]

备注/Memo

备注/Memo:
基金项目:陕西省中医药管理为中医科研项目中医临床研究课题(JCMS045)
通讯作者:任小朋,主任医师?E-mail:1055613232@qq.com
更新日期/Last Update: 2020-11-26