[1]李志超 谭国庆 徐展望 薛海鹏.基于生物信息学探讨甲状腺功能亢进 与骨质疏松间的作用关系[J].现代中医药,2021,(05):053-58.[doi:10.13424/j.cnki.mtcm.2021.05.009]
　LI Zhichao?TAN Guoqing?XU Zhanwang?XUE Haipeng.Study on The Relationship Between Hyperthyroidism and Osteoporosis Based on Bioinformatics[J].Modern Traditional Chinese Medicine,2021,(05):053-58.[doi:10.13424/j.cnki.mtcm.2021.05.009]
点击复制

基于生物信息学探讨甲状腺功能亢进 与骨质疏松间的作用关系
分享到：

《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年05期
页码:
053-58
栏目:
临床精粹
出版日期:
2021-09-23

文章信息/Info

Title:
Study on The Relationship Between Hyperthyroidism and Osteoporosis Based on Bioinformatics
文章编号:
1672-0571(2021)05-0053-06
作者:
李志超1 谭国庆2 徐展望2 薛海鹏2
1.山东中医药大学，山东 济南 250000；
2.山东中医药大学附属医院，山东 济南 250000
Author(s):
LI Zhichao1?TAN Guoqing2?XU Zhanwang2?XUE Haipeng2
1.Shandong University of Traditional Chinese Medicine，Jinan 250000,China； 2.Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan 250000,China
关键词:
骨质疏松; 甲状腺功能亢进; 生物信息学; 疾病间作用关系
Keywords:
Osteoporosis; Hyperthyroidism; Bioinformatics; Relationship between diseases
分类号:
R581.1?
DOI:
10.13424/j.cnki.mtcm.2021.05.009
文献标志码:
A
摘要:
目的 通过生物信息学相关技术方法探讨甲状腺功能亢进与骨质疏松之间的相互关系。方法 分别以骨质疏松及甲状腺功能亢进为关键词在相关疾病数据库(Disgenet、TTD、OMIM、Drugbank、Genecards)中筛选相关基因，去重整合后将两组预测靶基因进行映射得到关键靶点，通过STRING网站获得靶点PPI互相作用网络，根据degree值筛选出核心靶点并通过 DAVID 数据库进行GO富集分析以及 KEGG 通路分析。结果 通过相关疾病数据库搜索筛选出与骨质疏松症和甲状腺功能亢进相关靶点分别为875个和221个，映射出关键靶点99个，以degree≥35筛选出核心靶点16个并导入DAVID数据库获得44项GO富集结果（P<0.05）及45条KEGG信号通路（P<0.05）。结论 INS、IL6、ALB、TNF、IGF1等核心靶点作用于Jak-STAT通路、PI3K-Akt通路、HIF-1通路、细胞因子和细胞因子受体相互作用通路、IBD通路等多条信号通路同时对骨质疏松及甲状腺功能亢进起到调控作用，两种复杂代谢性疾病虽然各自包含众多作用靶点，但仍在分子机制上有着密切联系。应用生物信息学具有较高的置信度和参考价值，为探索疾病间关系提供了新方向与新思路。
Abstract:
Objective To explore the relationship between hyperthyroidism and osteoporosis by bioinformatics related techniques.Methods Relevant genes were screened in the related disease database (Disgenet,TTD,OMIM,Drugbank,Genecards) with osteoporosis and hyperthyroidism as key words，After de integration，the two groups of predicted target genes were mapped to obtain the key targets，the target PPI interaction network was obtained through the STRING website，the core targets were screened according to the degree value，and the go enrichment analysis and KEGG pathway analysis were carried out through the DAVID database.Results 875 and 221 targets related to osteoporosis and hyperthyroidism were screened through the search of related disease database，and 99 key targets were mapped.16 core targets were screened by degree ≥35 and imported into DAVID database.44 go enrichment results (P<0.05) and 45 KEGG signal pathways (P<0.05) were obtained.Conclusion INS，IL6，ALB，TNF，IGF1 and other core targets act on JAK STAT pathway，PI3K-Akt pathway，HIF-1 pathway，cytokine and cytokine receptor interaction pathway，IBD pathway and other signal pathways，and regulate osteoporosis and hyperthyroidism at the same time.Although the two complex metabolic diseases contain many targets，But it is still closely related to the molecular mechanism.Applied bioinformatics has high confidence and reference value，which provides a new direction and new ideas for exploring the relationship between diseases.

参考文献/References:

［1］Williams GR,Bassett JHD.Thyroid diseases and bone health\[J\].Journal of Endocrinological Investigation,2018,41(1):99-109.
［2］王晓平,段丽菊,陈晓白,等.五指毛桃水提液体外抗菌作用的实验研究\[J\].时珍国医国药,2010,21(7)：1692-1693.Bassett JH,Williams GR.Role of thyroid hormones in skeletal development and bone maintenance\[J\].Endocrine Reviews,2016,37(2):135-187.
［3］时鹤,崔岱.自身免疫性甲状腺疾病与骨代谢\[J\].中华骨质疏松和骨矿盐疾病杂志,2020,13(1):68-72.
［4］Wang QQ,Zhang BB,Xu YL,et al.The relationship between serum osteocalcin concentration and glucose metabolism in patients with type 2 diabetes mellitus\[J\].International Journal of Endocrinology,2013,2013:842598．
［5］Williams JP,Blair HC,Mcdonald JM,et al.Regulation of osteoclastic bone resorption by glucose\[J\].Biochemical and Biophysical Research Communications,1997,235(3):646-651．
［6］刘茜，王菊梅，董林，等.糖化血红蛋白联合空腹血糖检测在甲状腺功能亢进伴糖代谢异常患者中的诊断价值\[J\].中国糖尿病杂志，2018,26(2):107-111.
［7］邹冬梅,冯平.血清TSH、25(OH)D、TNF-α在糖尿病患者中的水平变化及与骨质疏松的相关性\[J\].国际检验医学杂志,2020,41(12):1520-1523.
［8］Marcocci C,Leo M,Altea MA.Oxidative stress in Graves&apos；disease\[J\].European Thyroid Journal,2012,1(2):80-87.
［9］吴世木,谢光云.甲状腺功能失调患者血清IL-6检测的临床意义\[J\].华夏医学,2000,13(2):142-143.
［10］卢亚敏,张明明,李天沛.老年甲亢性肝损害患者炎症因子的变化及意义\[J\].中国老年学杂志,2016,36(20):5036-5037. 李哲立.血清白蛋白、血红蛋白与骨质疏松的关系\[D\].福州:福建医科大学,2016.
［11］潘伟,唐华.1280例甲亢患者血液生化指标变化的探讨\[J\].现代诊断与治疗,2013,24(8):1804-1805.
［12］陈立业,蔡迎峰,刘保新,等.补肾活血方对绝经后骨质疏松症患者JAK/STAT信号通路的影响\[J\].现代医院,2020,20(6):895-897，902.
［13］Park E S.Involvement of JAK/STAT in the thyrotropin signaling pathway\[J\].Molecular endocrinology (Baltimore，Md.),2000,14(5).
［14］史东梅,董明,陆颖,等.PI3K/Akt信号通路与骨破坏：问题与机制\[J\].中国组织工程研究,2020,24(23):3716-3722.
［15］徐金玲,赵林双,王敏.血管紧张素Ⅱ1型受体自身抗体在甲亢大鼠心肌肥大中的作用及其与PI3K/Akt信号通路的关系\[J\].第二军医大学学报,2014,35(2):154-160
［16］张洁,王思瑶,陆春晖,等.雷公藤多苷调控PI3K/AKT/mTOR通路对Graves病小鼠甲状腺功能和氧化应激状态的作用研究\[J\].实用药物与临床,2020,23(8):688-692.
［17］Neri S.Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications:A Fundamental Biosafety Aspect\[J\].International Journal of Molecular Sciences,2019,20(10):E2406.
［18］Qu B,Hong Z，Gong K,et al.Inhibitors of Growth 1b Suppresses Peroxisome Proliferator-Activated Receptor-β/δ Expression Through Downregulation of Hypoxia-Inducible Factor 1α in Osteoblast Differentiation\[J\].DNA and Cell Biology,2016,35(4) :184-191.
［19］樊丽娅,施秉银,张煜.Graves甲亢患者治疗前后血清人HIF-1α及EPO水平的变化\[C\]//中华医学会第十二次全国内分泌学学术会议论文集.西安,2013:335.
［20］嘉士健,雷行华,廖建坤.浅谈控制炎症性肠病在治疗骨质疏松症中的重要作用\[J\].中医临床研究,2015,7(2):125-126.
［21］Paschou SA,Palioura E,Kothonas F,et al.The effect of anti-TNF therapy on thyroid function in patients with inflammatory bowel disease\[J\].Endocrine Journal,2018,65(11):1121-1125.

相似文献/References:

[1]覃 瑶** 黄崇刚 莫宗成 王 欣 王 敏 黄文涛 罗维早 ***.不同比例淫羊藿、蛇床子及川续断提取物配方的抗骨质疏松模型活性研究[J].现代中医药,2017,(06):122.[doi:10.13424/j.cnki.mtcm.2017.06.043]
　Qin Yao Huang Chonggang Mo Zongcheng Wang Xin Wang Min Huang Wentao Luo Weizao.Activity Study on Anti － osteoporosis Model with Different Proportions of Epimedium，Fructus Cnidii and Dipsacus Asper Extracts[J].Modern Traditional Chinese Medicine,2017,(05):122.[doi:10.13424/j.cnki.mtcm.2017.06.043]

备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目(81473709)；曹贻训全国名老中医传承工作室建设项目（国中医药人教函\[2018\]134号）；徐展望山东省名老中医药专家传承工作室建设项目(山东省卫生健康委员会，鲁卫函\[2019\]92号)
更新日期/Last Update: 2021-09-24