[1]赖晶 倪琳 蔺瑞丽 靳婉君 刘富强 宋平顺 马萧 滕宝霞.基于ITS序列PCRRFLP鉴别 北柴胡混伪品藏柴胡[J].现代中医药,2023,(05):105-112.[doi:10.13424/j.cnki.mtcm.2023.05.020]
　LAI Jing NI Lin LIN Ruili JIN Wanjun LIU Fuqiang SONG Pingshun MA Xiao TENG Baoxia.Identification of Mixed and Counterfeit Zang Chaihu from Bei Chaihu Based on ITS Sequence PCR-RFLP[J].Modern Traditional Chinese Medicine,2023,(05):105-112.[doi:10.13424/j.cnki.mtcm.2023.05.020]
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基于ITS序列PCRRFLP鉴别 北柴胡混伪品藏柴胡
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年05期
页码:
105-112
栏目:
出版日期:
2023-09-20

文章信息/Info

Title:
Identification of Mixed and Counterfeit Zang Chaihu from Bei Chaihu Based on ITS Sequence PCR-RFLP
文章编号:
1672-0571(2023)05-0105-08
作者:
赖晶 倪琳 蔺瑞丽 靳婉君 刘富强 宋平顺 马萧 滕宝霞
甘肃省药品检验研究院/国家中药材及饮片质量控制重点实验室，甘肃 兰州 730013
Author(s):
LAI Jing NI Lin LIN Ruili JIN Wanjun LIU Fuqiang SONG Pingshun MA Xiao TENG Baoxia
Gansu Provincial Institute for Drug Control/National Key Laboratory for Quality Control of Traditional Chinese Medicine and Dried Pieces，Lanzhou 730013,China
关键词:
关键词：藏柴胡; 北柴胡; ITS; PCR-RFLP; 分子鉴定
Keywords:
Key words:Zang Chaihu; Bei Chaihu; ITS; PCRRFLP; Molecular identification
分类号:
R282.5
DOI:
10.13424/j.cnki.mtcm.2023.05.020
文献标志码:
A
摘要:
摘 要：目的 基于ITS序列用PCR-RFLP方法鉴别北柴胡药材掺伪藏柴胡的方法。方法 分析并筛选出藏柴胡特有的限制性内切酶AseⅠ，用Primer Premier 5.0设计特异性引物，对引物PCR扩增条件和酶切试验进行优化，并对该方法的准确性进行了考察。结果 PCR扩增的目的片段为331 bp，且建立的PCR反应方法对不同的酶均具有适应性。限制性内切酶Ase Ⅰ将藏柴胡切成79 bp和252 bp两个片段，而北柴胡及其他柴胡均不能被酶切，且掺伪检出限为1%。结论 PCR-RFLP方法实现了准确鉴别北柴胡中的混伪品藏柴胡。
Abstract:
Abstract：Objective To identify the adulteration of Bupleurum chinense with Zang Bupleurum chinense based on ITS sequence using PCRRFLP method.Methods Analysis and screening were conducted to identify the specific restriction endonuclease Ase Ⅰ in Tibetan Chaihu.Primer Premier 5.0 was used to design specific primers，optimize the PCR amplification conditions and enzyme digestion test，and evaluate the accuracy of this method.Results The target fragment amplified by PCR was 331 bp，and the established PCR reaction method was adaptable to different enzymes.Restrictive endonuclease Ase Ⅰ cleaves Zang Bupleurum into 79 bp and 252 bp fragments，while Northern Bupleurum and other Bupleurum cannot be cleaved，and the detection limit for adulteration is 1%.Conclusion The PCR-RFLP method has achieved accurate identification of the counterfeit Zang Chaihu in Beichaihu.

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备注/Memo

备注/Memo:
基金项目：国家药监局中药材及饮片质量控制重点实验室项目(2021GSMPA-KL03)
更新日期/Last Update: 2023-09-22