[1]张晶 吴晓晨 张家旺 谢雨婕 钟华胜.全真一气汤对肾气虚型哮喘大鼠转录因子FOXP3/RORγt及Th17/Treg免疫失衡的影响[J].现代中医药,2023,(06):096-102.[doi:10.13424/j.cnki.mtcm.2023.06.020]
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全真一气汤对肾气虚型哮喘大鼠转录因子FOXP3/RORγt及Th17/Treg免疫失衡的影响()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年06期
页码:
096-102
栏目:
出版日期:
2023-12-08

文章信息/Info

文章编号:
1672-0571(2023)06-0096-07
作者:
张晶1 吴晓晨1 张家旺1 谢雨婕1 钟华胜2
1.福建中医药大学附属第二人民医院,福建 福州 350003;
2.龙岩市上杭县医院,福建 上杭 364200
关键词:
关键词:全真一气汤支气管哮喘辅助型T细胞17/调节性T细胞维甲酸相关孤独核受体-γt叉头状转录因子细胞因子
分类号:
R256.12
DOI:
10.13424/j.cnki.mtcm.2023.06.020
文献标志码:
A
摘要:
摘 要:目的 观察全真一气汤对肾气虚型哮喘大鼠Th17/Treg及相关细胞因子(IL-6、IL-17、IL-10、IL-35)和转录因子维甲酸相关孤独核受体-γt(OrPhan nuclear recePtor gamma t,RORγt)、叉头状转录因子(forkhead transcriPtion factor P3,FOXP3)的影响,探讨全真一气汤调控哮喘气道炎症的机制。方法 将24只SD雄性大鼠随机分为空白组、模型组、全真一气汤低剂量治疗组(8.33g·kg-1)和全真一气汤高剂量治疗组(33.32 g·kg-1),每组6只。模型组与低、高剂量治疗组予卵蛋白1mg、氢氧化铝凝胶10 mg的混悬液1 mL致敏,1%的卵清蛋白超声雾化吸入以激发哮喘,进行惊恐刺激,负重游泳直至力竭等进行肾气虚型哮喘大鼠造模,造模成功后开始药物干预。空白组和模型组予生理盐水3 mL灌服,治疗组按低、高剂量予全真一气汤3 mL灌服,每日1次,连服30 d。实时荧光定量聚合酶链式反应(Real time Quanti tative PCR,qRT-PCR)检测各组大鼠肺组织中FOXP3、RORγt mRNA水平,流式细胞术检测外周血Th17、Treg细胞百分比,酶联免疫吸附实验(Enzyme Linked Immunosorbent Assay,ELISA)检测血清IL-6、IL-17、IL-10、IL-35细胞因子含量。结果 与空白组相比,模型组血Th17细胞比例、血清IL-6、IL-17含量及相关肺组织中RORγt mRNA和蛋白表达水平显著升高(P<0.05),模型组Treg细胞比例、血清IL-10、IL-35含量及相关肺组织中FOXP3 mRNA和蛋白表达水平明显降低(P<0.05);与模型组相比,全真一气汤低、高剂量组血Th17细胞百分比、血清IL-6、IL-17含量及肺组织RORγt mRNA和蛋白的表达明显减少(P<0.05),高剂量组血Treg细胞百分比显著升高(P<0.05),但全真一气汤低剂量组血Treg细胞百分比无显著变化(P>0.05),高剂量组血清IL-10含量增加(P<0.05),全真一气汤低剂量组IL-10含量亦增加,但差异无统计学意义(P>0.05),两组血清IL-35含量、FOXP3 mRNA及蛋白的表达同时明显升高(P<0.05);与全真一气汤低剂量组相比,高剂量组Th17细胞百分比明显降低(P>0.05),IL-17亦降低(P>0.05),IL-6含量及RORγt表达减少(P<0.05),高剂量组IL-35含量及Treg相关的FOXP3表达增加(P<0.05)。结论 全真一气汤低剂量组、全真一气汤高剂量组全真一气汤均可降低Th17细胞及相关促炎因子IL-6、IL-17的含量,抑制RORγt的表达;高剂量组可以升高Treg细胞百分比,而全真一气汤低剂量组对Treg细胞百分比影响则不明显,但都可以上调FOXP3的表达,促进相关抑炎因子IL-10、IL-35分泌。摘 要:目的 观察全真一气汤对肾气虚型哮喘大鼠Th17/Treg及相关细胞因子(IL-6、IL-17、IL-10、IL-35)和转录因子维甲酸相关孤独核受体-γt(OrPhan nuclear recePtor gamma t,RORγt)、叉头状转录因子(forkhead transcriPtion factor P3,FOXP3)的影响,探讨全真一气汤调控哮喘气道炎症的机制。方法 将24只SD雄性大鼠随机分为空白组、模型组、全真一气汤低剂量治疗组(8.33g·kg-1)和全真一气汤高剂量治疗组(33.32 g·kg-1),每组6只。模型组与低、高剂量治疗组予卵蛋白1mg、氢氧化铝凝胶10 mg的混悬液1 mL致敏,1%的卵清蛋白超声雾化吸入以激发哮喘,进行惊恐刺激,负重游泳直至力竭等进行肾气虚型哮喘大鼠造模,造模成功后开始药物干预。空白组和模型组予生理盐水3 mL灌服,治疗组按低、高剂量予全真一气汤3 mL灌服,每日1次,连服30 d。实时荧光定量聚合酶链式反应(Real time Quanti tative PCR,qRT-PCR)检测各组大鼠肺组织中FOXP3、RORγt mRNA水平,流式细胞术检测外周血Th17、Treg细胞百分比,酶联免疫吸附实验(Enzyme Linked Immunosorbent Assay,ELISA)检测血清IL-6、IL-17、IL-10、IL-35细胞因子含量。结果 与空白组相比,模型组血Th17细胞比例、血清IL-6、IL-17含量及相关肺组织中RORγt mRNA和蛋白表达水平显著升高(P<0.05),模型组Treg细胞比例、血清IL-10、IL-35含量及相关肺组织中FOXP3 mRNA和蛋白表达水平明显降低(P<0.05);与模型组相比,全真一气汤低、高剂量组血Th17细胞百分比、血清IL-6、IL-17含量及肺组织RORγt mRNA和蛋白的表达明显减少(P<0.05),高剂量组血Treg细胞百分比显著升高(P<0.05),但全真一气汤低剂量组血Treg细胞百分比无显著变化(P>0.05),高剂量组血清IL-10含量增加(P<0.05),全真一气汤低剂量组IL-10含量亦增加,但差异无统计学意义(P>0.05),两组血清IL-35含量、FOXP3 mRNA及蛋白的表达同时明显升高(P<0.05);与全真一气汤低剂量组相比,高剂量组Th17细胞百分比明显降低(P>0.05),IL-17亦降低(P>0.05),IL-6含量及RORγt表达减少(P<0.05),高剂量组IL-35含量及Treg相关的FOXP3表达增加(P<0.05)。结论 全真一气汤低剂量组、全真一气汤高剂量组全真一气汤均可降低Th17细胞及相关促炎因子IL-6、IL-17的含量,抑制RORγt的表达;高剂量组可以升高Treg细胞百分比,而全真一气汤低剂量组对Treg细胞百分比影响则不明显,但都可以上调FOXP3的表达,促进相关抑炎因子IL-10、IL-35分泌。

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备注/Memo

备注/Memo:
基金项目:福建省自然基金面上项目(2020J01245)
更新日期/Last Update: 2023-11-23