[1]许伟英孙永宁** 王子杨 刘美志 屠艳婕 曹燕折哲.黄芩咳喘散治疗支气管哮喘临床研究和网络药理学效应机制探讨[J].现代中医药,2024,(05):051-61.[doi:10.13424/j.cnki.mtcm.2024.05.011]
　XU WeiyingSUN Yongning WANG Ziyang LIU MeizhiTU YanjieCAO YanZHE Zhe.Clinical Study and Network Pharmacology MechanismExplorationof Huangqin Kechuan San in theTreatment of Bronchial Asthma[J].Modern Traditional Chinese Medicine,2024,(05):051-61.[doi:10.13424/j.cnki.mtcm.2024.05.011]
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黄芩咳喘散治疗支气管哮喘临床研究和网络药理学效应机制探讨()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年05期
页码:
051-61
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Clinical Study and Network Pharmacology MechanismExplorationof Huangqin Kechuan San in theTreatment of Bronchial Asthma
文章编号:
1672-0571(2024)04-0056-04
作者:
许伟英1孙永宁1** 王子杨2 刘美志2 屠艳婕2 曹燕1折哲1
1.上海中医药大学附属市中医医院，上海 200071；
2.上海中医药大学研究生院，上海 201203
Author(s):
XU Weiying1SUN Yongning1 WANG Ziyang2 LIU Meizhi2TU Yanjie2CAO Yan1ZHE Zhe1
1.Affiliated Municipal Hospital of Traditional Chinese Medicine of Shanghai University of Chinese Medicine，Shanghai 200071，China；
2.Graduate School of Shanghai University of Chinese Medicine，Shanghai 201203，China
关键词:
关键词：黄芩咳喘散; 支气管哮喘; 临床疗效; 网络药理学靶点; 分子对接
Keywords:
Key words:Huangqin Kechuan San; Bronchial asthma; Clinical efficacy; Network pharmacology targets; Molecular docking
分类号:
R256.11
DOI:
10.13424/j.cnki.mtcm.2024.05.011
文献标志码:
A
摘要:
摘 要：目的 基于网络药理学研究黄芩咳喘散防治支气管哮喘的有效性及其效应物质探讨。方法 选取符合纳入标准的患者共94例，随机分为治疗组和对照组各47例。所有患者均接受常规药物治疗。治疗组给予黄芩咳喘散三伏贴，对照组给予其模拟剂三伏贴。对比两组患者治疗前后中医症候积分评分情况、ACT评分、半年内疾病发作次数、血清嗜酸性粒细胞（EOS）等情况。基于网络药理学和分子对接等探讨黄芩咳喘散与支气管哮喘可能的效应物质及作用机制。结果 数据显示：①治疗组患者临床治疗总有效率高于对照组(P＜0.05)；②治疗组患者咳嗽气促、喘息咯痰、食少便溏、神疲乏力单项症状评分、ACT评分、半年内疾病发作次数均低于对照组(均P＜0.05)；③两组患者EOS水平均降低，且治疗组各指标值明显低于对照组(均P＜0.05)；④网络药理学筛选黄芩咳喘散有效活性成分潜在作用靶点与支气管哮喘的交集靶点26个，关键有效成分包括四羟基黄酮、亚油酸乙酯、棕榈酸等，涉及核心靶点包括SIRT1、STAT3、ESR1、PPARA等，可能涉及免疫、脂肪酸代谢、激素类反应、细胞坏死因子反应等生物过程。⑤分子对接显示黄芩咳喘散中有效活性成分四羟基黄酮（5，7，2′，6′-Tetrahydroxyflavone）通过氢键分别与支气管哮喘关键靶点SIRT1的氨基酸ILE411、GLU410、LYS408、SER370、LYS375，与STAT3的氨基酸ASP334、LYS574，与ESR1的氨基酸LEU346及PPARA的氨基酸MET320、LEU331、THR334结合。结论 黄芩咳喘散治疗支气管哮喘肺脾气虚证患者具有可靠的临床疗效，在改善临床症状、减少疾病发作次数等具有积极作用。经网络药理学分析黄芩咳喘散可能通过SIRT1、STAT3、ESR1等靶点作用于PI3K-Akt、JAK-STAT、NF-κB等通路从减轻气道炎症反应、抑制气道重塑等方面发挥治疗作用，进一步分子对接后提示该药的有效活性成分四羟基黄酮与哮喘疾病靶点SIRT1、STAT3、ESR1及PPARA具有良好的结合能力。
Abstract:
Abstract：Objective Based on network pharmacology，this study aims to investigate the effectiveness of Huangqin Kechuan San in preventing and treating bronchial asthma，as well as to explore its effective substances.Methods A total of 94 patients who met the inclusion criteria were randomly divided into a treatment group and a control group，with 47 patients in each group.All patients received routine medication treatment.The treatment group was given Huangqin Kechuan San Sanfu Paste，while the control group was given its analog Sanfu Paste.Compare the TCM symptom score，ACT score，frequency of disease attacks within six months，and serum eosinophil count (EOS) between two groups of patients before and after treatment.Exploring the possible effector substances and mechanisms of Huangqin Kechuan San in relation to bronchial asthma based on network pharmacology and molecular docking.Results The data showed that：① The total effective rate of clinical treatment in the treatment group was higher than that in the control group (P<0.05)；② The single symptom scores，ACT scores，and frequency of disease attacks within six months in the treatment group were lower than those in the control group (all P<0.05)，including cough，shortness of breath，wheezing，phlegm production，reduced appetite，loose stools，and fatigue.The EOS levels of both groups of patients decreased，and the values of various indicators in the treatment group were significantly lower than those in the control group (both P<0.05)；④Network pharmacology screening identified 26 potential targets of active ingredients in Huangqin Kechuan San that intersect with bronchial asthma.Key active ingredients include tetrahydroxyflavonoids，ethyl linoleate，palmitic acid，etc.Core targets include SIRT1，STAT3，ESR1，PPARA，etc.，which may involve biological processes such as immunity，fatty acid metabolism，hormone reactions，and cell necrosis factor reactions.⑤ Molecular docking showed that the effective active ingredient tetrahydroxyflavone (5，7，2′，6′-Tetrahydroxyflavone) in Huangqin Kechuan San was hydrogen bonded to the amino acids ILE411 and ILE411 of the key target SIRT1 in bronchial asthma GLU410、LYS408、SER370、LYS375.It binds to the amino acids ASP334 and LY2574 of STAT3，the amino acid LEU346 of ESR1，and the amino acids MET320，LEU331，and THR334 of PPARA.Conclusion Huangqin Kechuan San has reliable clinical efficacy in treating patients with bronchial asthma and lung spleen deficiency syndrome，and has a positive effect in improving clinical symptoms and reducing the frequency of disease attacks.According to network pharmacology analysis，Huangqin Kechuan San may exert therapeutic effects by targeting PI3K Akt，JAK-STAT，NF-κΒ，and other pathways through SIRT1，STAT3，ESR1，etc.，to alleviate airway inflammation and inhibit airway remodeling.Further molecular docking suggests that the active ingredient of the drug，tetrahydroxyflavone，has good binding ability with asthma disease targets SIRT1，STAT3，ESR1，and PPARA.

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备注/Memo

备注/Memo:
基金项目：上海市卫生健康委员会科研课题（202040261）
更新日期/Last Update: 2024-09-20