[1] Wang Yuanchun Mu Meiling Wang Xisheng Yang uifeng Tian Yali Hui Jianrong,.Effect of Elm Oil on Skin Histopathology and P53 Gene Expression in Rats with Radioactive Skin Injury Induced by β - rays [J]. Modern Traditional Chinese Medicine, 2018, (02): 83-86. [doi: 10.13424/j.cnki.mtcm.2018.02.030]
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Modern Traditional Chinese Medicine[ISSN: 1006-6977/ CN: 61-1281/TN]
Volumes & Issues:
2018 02
pagination:
83-86
Column:
Publication date:
2018-03-10
- Article number:
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1672-0571(2018)02-0083-04
- Author(s):
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Wang Yuanchun1 Mu Meiling2 Wang Xisheng1 Yang Ruifeng1 Tian Yali1 Hui Jianrong3
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1. Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China; 2. The Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China; 3. Shaanxi University of Chinese Medicine,Xianyang 712046,China
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- Keywords:
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elm oil; radioactive skin injury; P53 gene
- ZTFLH:
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R285.5
- DOI:
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10.13424/j.cnki.mtcm.2018.02.030
- Code:
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A
- Abstract:
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Objective: To evaluate the effect of elm oil in the prevention and treatment of radioactive skin injury and probe into its pathological mechanism as well as its effect on P53 gene expression. Method: 40 female SD rats’buttock skin hair were removed by barium sulfide,10 were randomly selected as the blank group,the remaining 30 anesthetized rats were irradiated with 6 MeV linear accelerator electron beam at an absorbed dose rate of 225 cGy / min with a cumula- tive dose of 45 Gy. Then the rats were randomly divided into model group,treatment group and control group with 10 rats in each,and given corresponding treatment. The changes of the skin were observed and the histopathological changes were observed by light microscope,the expression of P53 gene was detected by immunohistochemistry. Result: Skin damage occurred in the irradiated rats,the lightest was in the treatment group,the heaviest in the model group and the middle in the control group. On the 14th day of irradiation,obvious pathological changes of the irradiated rat skin appeared,hair follicle epithelial damage and large amount of inflammatory cell infiltration were observed. On the 30th day of irradiation, part of the wound repaired in both the treatment group and the control group,with the treatment group more obvious. Fi- broblast and collagenous fiber proliferation were observed. The model group had significant skin lesions,epidermis and dermis superficial tissue necrosis,intradermal edema,telangiectasia,hemorrhage,extensive neutrophil and lymphocyte infiltration. The expression of P53 gene in the irradiated rat skin was highly expressed on the 14th day,and significantly decreased on the 30th day in the treatment group compared with the model group and the control group ( P < 0. 05) . Conclusion: elm oil has a significant preventive and curative effect on acute radioactive skin injury in rats,and its mech- anism may be related to promoting proliferation of fibroblasts and collagen fibers,reducing the expression of P53 gene and inhibiting cell apoptosis.