[1] HAN Sirong QU Jie YANG Jingfeng YANG Jun TAN Yingying LI Xiaohui CHEN Liming,.Effects of Didang Qigui Decoction on PI3K and Akt Gene and Protein in Liver Tissue of Type 2 Diabetic Rats [J]. Modern Traditional Chinese Medicine, 2022, 1(02): 20-24. [doi: 10.13424/j.cnki.mtcm.2022.02.004]
Effects of Didang Qigui Decoction on PI3K and Akt Gene and Protein in Liver Tissue of Type 2 Diabetic Rats
- Article number:
- 1672-0571(2022)02-0020-06
- Keywords:
- Key words：Didang Qigui decoction; Type 2 diabetes mellitus; PI3K/Akt; Liver; Insulin resistance
- ZTFLH:
- R259
- Code:
- A
- Abstract:
- Abstract：Objective To study the effects of Didang Qigui decoction on PI3K and Akt gene and protein in liver of type 2 diabetic rats.Methods High glucose and high-fat diet combined with intraperitoneal injection of small dose of streptozotocin were used to establish type 2 diabetes mellitus rats.They were randomly divided into model group，high dose group of Didang Qigui decoction (group DQG),Didang Qigui decoction constant group (group DQZ)，metformin group (group Met),Didang Qigui decoction plus metformin group (DQZX group).After 6 weeks of gavage，the levels of fasting blood glucose (FBG)，glycosylated hemoglobin (HbA1c) and liver insulin were measured；RT-PCR was used to detect the expression of PI3K and Akt mRNA in liver tissue；The expression of PI3K and Akt protein in liver tissue was detected by western blot.Results The levels of FBG，HbA1c and insulin in liver tissue decreased in different degrees in Didang Qigui decoction constant group and Didang Qigui decoction plus metformin group (P<0.05 or P<0.01)，and the expressions of PI3K and Akt genes and proteins in liver tissue increased significantly (P<0.01).Conclusion Didang Qigui decoction can regulate the expression of PI3K and Akt in the insulin signaling pathway of type 2 diabetic rats，regulate glucose metabolism disorder and improve insulin resistance.
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Memo
基金项目：陕西省教育厅科研项目（19JK0227）；伤寒学与经方辨治疑难病一流创新团队（2019-QN04）；陕西省伤寒学与经方治疗辨治疑难病研究室项目