[1]刘臖 胡雨彤 刘江涛 仝警安 周海燕.马齿苋多糖对高糖诱导的人晶状体上皮细胞发生上皮间质转化的影响[J].现代中医药,2021,(01):015-19,41.[doi:10.13424/j.cnki.mtcm.2021.01.004]
　LIU Jun,HU Yu - tong,LIU Jiang - tao,et al.Effects of Portulaca Oleracea Polysaccharide on Epithelial Mesenchymal Transition of Human Lens Epithelial Cells Induced by High Glucose[J].Modern Traditional Chinese Medicine,2021,(01):015-19,41.[doi:10.13424/j.cnki.mtcm.2021.01.004]
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马齿苋多糖对高糖诱导的人晶状体上皮细胞发生上皮间质转化的影响()
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
015-19,41
栏目:
出版日期:
2023-11-20

文章信息/Info

Title:
Effects of Portulaca Oleracea Polysaccharide on Epithelial Mesenchymal Transition of Human Lens Epithelial Cells Induced by High Glucose
文章编号:
1672-0571(2021)01-0015-06
作者:
刘臖1 胡雨彤2 刘江涛3 仝警安1 周海燕4
1.陕西中医药大学附属医院,陕西咸阳712000;
2.西安航天总医院,陕西西安710100;
3.陕西中医药大学,陕西咸阳712046;
4.陕西省人民医院陕西西安710068
Author(s):
LIU Jun 1;  HU Yu - tong 2;  LIU Jiang - tao 3;  TONG Jing - an 1;  ZHOU Hai - yan 4
1. Affiliated Hospital of Shaanxi University of traditional Chinese medicine, Shaanxi Xianyang 712000, China;
2. Xi’ an Aerospace general hospital, Xi’ an 710100, China;
3. Shaanxi University of traditional Chinese medicine, Shaanxi Xianyang 712046, China;
4. People’ s Hospital of Shaanxi Province, Xi’ an 710068, China
关键词:
马齿苋多糖; 人晶状体上皮细胞; 高糖; 上皮间质转化
Keywords:
Portulaca oleracea polysaccharide;  Human lens epithelial cells;  High glucose;  Epithelial mesenchymal transition
分类号:
R96.5
DOI:
10.13424/j.cnki.mtcm.2021.01.004
文献标志码:
A
摘要:
目的 观察马齿苋多糖对高糖作用下永生系人晶状体上皮细胞(SRA01/04)上皮间质转化的影响。方法 体外培养SRA01/04细胞,将传代的细胞随机分为正常对照组、高糖组、高糖+马齿苋多糖组,分别用含5.5mmol/L葡萄糖、30mmol/L葡萄糖、30mmol/L葡萄糖+0.5mg/mL马齿苋多糖的培养基培养48h,于培养的0h、6h、12h、24h、48h在倒置显微镜下分别观察三组的细胞形态学变化;48h后Western-blot检测细胞ZO-1、E-cadherin及α-SMA、Vimentin的蛋白表达变化，结果与对照组相比,高糖组细胞随时间延长细胞变长出现成纤维改变,细胞数量也逐渐减少;高糖联合马齿苋多糖组与正常组的形态变化基本趋于一致,没有随着时间延长而发生明显的梭形改变，与对照组相比,高糖组ZO-1、E-cadherin表达下降(P<0.01),α-SMA和Vimentin的表达上升(P<0.01);高糖联合马齿苋多糖组ZO-1、E-cadherin表达下降(P<0.05),α-SMA和Vimentin的表达上升(P<0.05)?与高糖组相比,高糖联合马齿苋多糖组ZO-1、E-cadherin表达上升(P<0.01),α-SMA和Vimentin的表达下降(P<0.01)。结论 高糖可以诱导晶状体上皮细胞发生上皮间质转化,马齿苋多糖可以抑制这一过程。
Abstract:
Objective: To observe the effects of Portulaca oleracea polysaccharide on epithelial mesenchymal transition of immortalized human lens epithelial cells ( SRA01 / 04) induced by high glucose. Methods: SRA01 / 04 cells were cultured in vitro and randomly divided into normal control group, high glucose group and high glucose + Portulaca oleracea polysaccharide group. The cells were cultured in 0. 5 mg / ml Portulaca oleracea polysaccharide medium for 48 h, and the morphological changes of the three groups were observed under the inverted microscope at 0 h, 6 h, 12 h, 24h and 48 h. The protein expression of ZO -1, E - cadherin, α - SMA and vimentin were detected by Western blot after 48 h. Results: Compared with the control group, the cells in the high glucose group grew longer, and the number of cells decreased gradually; the morphological changes of the high glucose combined with purslane polysaccharide group and the normal group were basically the same, and there was no obvious spindle shape change with time. Compared with the control group, the expression of ZO -1, E - cadherin in high glucose group was decreased ( P < 0. 01) , the expression of α- SMA and vimentin was increased ( P < 0. 01) ; the expression of ZO -1 and E - cadherin in high glucose combined with Portulaca polysaccharide group was decreased ( P < 0. 05) , and the expression of α - SMA and vimentin was increased ( P < 0. 05) . Compared with the high glucose group, the expression of ZO -1 and E - cadherin increased in the high glucose combined with Portulaca polysaccharide group ( P < 0. 01) , while the expression of α - SMA and vimentin decreased ( P < 0. 01) . Conclusion: High glucose can induce epithelial mesenchymal transition of lens epithelial cells, and Portulaca oleracea polysaccharide can inhibit this process.

参考文献/References:

[1] Drinkwater JJ, Davis WA, Davis TME. A systematic review of risk factors for cataract in type 2 diabetes[ J] . Diabetes Metab Res Rev, 2019,359( 1) e3073.
[2] Li X, Wang F, Ren M, Du M, et al. The effects of c -Src kinase on EMT signaling pathway in human lens epithelial cells associated with lens diseases[ J] . BMC Ophthalmol, 2019,19( 1) :219.
[3] Han ZH, Wang F, Wang FL, et al. Regulation of transforming growth factor β - mediated epithelial - mesenchymal transition of lens epithelial cells by c - Src kinase under high glucose conditions[ J] . Exp Ther Med, 2018, 16( 2) :1520 ? 1528.
[4] Elkhayat ES, Ibrahim SR, Aziz MA. Portulene, a new diterpene from Portulaca oleracea L[ J] . J Asian Nat Prod Res,2008,10( 11 -12) :1039 ? 1043.
[5] Zhao R, Zhang T, Zhao H, et al. Effects of Portulaca oleracea L. Polysaccharides on Phenotypic and Functional Maturation of Murine Bone Marrow Derived Dendritic Cells [ J] . Nutr Cancer, 2015,67( 6) :987 ? 993.[6] Nieto MA, Huang RY, Jackson RA, etal. EMT: 2016. Cell[ J] . 2016,166( 1) :21 ? 45.
[7] Eldred JA, Dawes LJ, Wormstone IM. The lens as a model for fibrotic disease[ J] . Philos Trans R Soc Lond B Biol Sci,2011,366( 1568) :1301 ? 1319.
[8] Li X, Wang F, Ren M, et al. The effects of c - Src kinase on EMT signaling pathway in human lens epithelial cells associated with lens diseases[ J] . BMC Ophthalmol,2019;19( 1) :219.
[9] Lu Q, Yang T, Zhang M, et al. Preventative effects of Ginkgo biloba extract ( EGb761) on high glucose – cultured opacity of rat lens[ J] . Phytother Res, 2014; 28( 5) : 767- 773.
[10] Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial - mesenchymal transition[ J] . Nat Rev Mol Cell Biol,2014,15( 3) :178 ? 196.
[11] Yang X, Yan Y, Li J, et al. Protective effects of ethanol extract from Portulaca oleracea L on dextran sulphate sodium - induced mice ulcerative colitis involving anti - inflammatory and antioxidant[ J] . Am J Transl Res, 2016, 8( 5) :2138 -2148.
[12] Kim JY, Oh HM, Kwak SC, et al. Purslane suppresses osteoclast differentiation and bone resorbing activity via inhibition of Akt/ GSK3β - c - Fos - NFATc1 signaling in vitro and prevents lipopolysaccharide - induced bone loss in vivo [ J] . Biol Pharm Bull,2015,38( 1) :66 ? 74.
[13]李玉萍,刘志勇,谢国秀,等.马齿苋多糖抗糖尿病作用的实验研究[J].天然产物研究与开发,2008,(5):813-815.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81600720)
通讯作者:周海燕,博士,副主任医师?E-mail:zhy2006614@163.com
更新日期/Last Update: 2021-01-21