[1]毛婷毛乐张屏张勇.硝酸银硅胶色谱法制备蒙药土木香中土木香内酯和异土木香内酯[J].现代中医药,2024,(03):094-98.[doi:10.13424/j.cnki.mtcm.2024.03.018]
MAO TingMAO LeZHANG PingZHANG Yong.Preparation of Tu Mu Xiang lactone and Iso Tu MuXiang lactone in Mongolian Medicine Tu Mu Xiangby Silver Nitrate Silica Gel Chromatography[J].Modern Traditional Chinese Medicine,2024,(03):094-98.[doi:10.13424/j.cnki.mtcm.2024.03.018]
点击复制
硝酸银硅胶色谱法制备蒙药土木香中土木香内酯和异土木香内酯(
)
《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
-
- 期数:
-
2024年03期
- 页码:
-
094-98
- 栏目:
-
- 出版日期:
-
2024-05-20
文章信息/Info
- Title:
-
Preparation of Tu Mu Xiang lactone and Iso Tu MuXiang lactone in Mongolian Medicine Tu Mu Xiangby Silver Nitrate Silica Gel Chromatography
- 文章编号:
-
1672-0571(2024)03-0094-05
- 作者:
-
毛婷1毛乐2张屏3张勇4
-
1.内蒙古包钢医院,内蒙古 包头 014010;
2.内蒙古科技大学包头医学院第三附属医院,内蒙 古包头 014010;
3.内蒙古医科大学,内蒙古 呼和浩特 010110;
4.内蒙古科技大学包头医学院第一附属医院,内蒙古 包头 014010
- Author(s):
-
MAO Ting1MAO Le2ZHANG Ping3ZHANG Yong4
-
1.Inner Mongolia Baogang Hospital,Inner Mongolia Baotou 014010,China;
2.Third Affiliated Hospital of BaotouMedical College,Inner Mongolia University of Science and Technology,Inner Mongolia Baotou 014010,China;
3.Inner Mongolia Medical Universit
-
- 关键词:
-
关键词:硝酸银硅胶色谱法; 土木香内酯; 异土木香内酯
- Keywords:
-
Key words:Silver nitrate silica gel chromatography; Tu Tu Xiang lactone; Iso Tu Mu Xiang lactone
- 分类号:
-
R93
- DOI:
-
10.13424/j.cnki.mtcm.2024.03.018
- 文献标志码:
-
A
- 摘要:
-
摘要:目的 探讨蒙药土木香中土木香内酯及异土木香内酯的制备方法。方法 采用硝酸银硅胶色谱法进行制备。结果 得到两个色泽优良、克数较大的结晶化合物,根据其理化性质及波谱技术鉴定为土木香内酯和异土木香内酯。结论 硝酸银硅胶色谱法对土木香内酯及异土木香内酯能实现满意分离,且产量大、纯度高、操作简单,是一种高效快速的制备方法,为土木香内酯及异土木香内酯的广泛应用提供精准可靠的参考资料。
- Abstract:
-
Abstract:Objective To explore the preparation methods of Tu Mu Xiang lactone and Iso Tu Mu Xiang lactone in Mongolian medicine.Methods The method was prepared using silver nitrate silica gel chromatography.Results As a result,two crystalline compounds with excellent color and large weight were obtained,and identified as Tu Mu Xiang lactone and Iso Tu Mu Xiang lactone based on their physical and chemical properties and spectroscopic techniques.Conclusion Silver nitrate silica gel chromatography can achieve satisfactory separation of Tu Mu Xiang lactone and Iso Tu Mu Xiang lactone,with high yield,high purity,and simple operation.It is an efficient and rapid preparation method,providing accurate and reliable reference materials for the widespread application of Tu Mu Xiang lactone and Iso Tu Mu Xiang lactone.
参考文献/References:
[1]吴明.土木香根化学成分研究与土木香倍半萜内酯的结构修饰[D].石家庄:河北医科大学,2010.
[2]赵永明.土木香化学成分的研究[D].石家庄:河北医科大学,2007.
[3]风云.土木香化学成分与药理作用研究进展[J].中国民族医药杂志,2021,27(12):48-49.
[4]ZHENG XQ,WU Z,XU J,et al.Bioactive sesquiterpenes from Inula helenium[J].Bioorganic chemist-ry,2021,114:105066.
[5]YANG CM,ZHANG LL,HUANG HK,et al.Alantolactone inhibits proliferation,metastasis and promotesapoptosis of human osteosarcoma cells by suppressing Wnt/β-catenin and MAPKs signaling pathway[J].Genes&Diseases,2022,09(2):466-478.
[6]YAN YY,ZHANG Q,ZHANG B,et al.Active ingredients of Inula helenium L.exhibits similar anti-cancer effects as isoalantolactone in pancreatic cancer cells[J].Natural Product Research:Formerly Natural Product Letters,2020,34(17):2539-2544.
[7]ZHANG B,ZENG JM,YAN YY.Ethyl acetate extract from Inula helenium L.inhibits the proliferation of pancreatic cancer cells by regulating the STAT3/AKT pathway[J].Molecular Medicine Reports,2018,17(4):5440-5448.
[8]欧阳长庚,卢文彪.利用硝酸银处理的硅胶色谱方法分离和提纯中药有效成分的思考[J].广州中医药大学学报,2004(4):319-322.
[9]张新新,杨洋,刘胜家,等.藏木香中土木香内酯和异土木香内酯的制备[J].中国医药工业杂志,2014,45(2):116-119.
[10]郭剑霞,张谨华,潘玉峰.硝酸银-硅胶柱层析法分离纯化华山松籽油亚油酸[J].食品工业,2020,41(9):226-231.
[11]王艳,周长远,杜爱玲.硅胶—硝酸银硅胶柱层析分离纯化β-倍半水芹烯[J].食品与机械,2016,32(5):165-167.
[12]程媛媛,杨传彬,赵佳,等.硝酸银改性硅胶柱色谱法纯化川芎精油中的藁本内酯[J].中国现代应用药学,2014,31(5):552-555.
[13]张良,刘美玲,董强,等.硝酸银硅胶柱分离纯化蛇鲻鱼油中的EPA和DHA[J].中国粮油学报,2011,26(12):63-68.
[14]PANKAJ P DARAMWAR,PRABHAKAR LAL SRIVASTAVA,BALARAMAN Priyadarshini.Preparative separation of α-and β-santalenes and (Z)-α-and(Z)-β-santalols using silver nitrate-impregnated silica gel medium pressure liquid chromatography and analysis of sandalwood oil[J].The Analyst,2012,137(19):4564-4570.
[15]黄汉昌,朱宏吉,姜招峰.银离子配位色谱法分离香茅次油中β-榄香烯的研究[J].无机化学学报,2009,25(3):433-438.
[16]周长远,王艳,杜爱玲.姜油树脂中β-倍半水芹烯的分离纯化[J].食品工业科技,2016,37(3):225-228.
[17]张卫民,翟元春,杨红军,等.硝酸银-硅胶柱层析法分离纯化核桃油中亚麻酸工艺研究[J].食品工业科技,2015,36(10):229-232.
[18]刘国宇,李静,王新莉,等.土木香中土木香内酯与异土木香内酯的分离鉴定[J].陕西农业科学,2011,57(4):9-10.
[19]王战国,赖先荣,肖莹莹,等.川木香质量标准研究[J].成都中医药大学学报,2006,29(1):54-56.
[20]胡春艳,闫媛博,鲍劲松,等.土木香薄层色谱鉴别方法的探讨[J].北方药学,2009,06(1):1-6.
[21]马静,宋毅,王曙,等.藏木香的生药学研究及质量标准制订[J].中药材,2017,40(6):1384-1388.
[22]ZHAO YM,ZHANG ML,SHI QW,et al.Study on the chemical components of the Inula helenium[J].Natural Product Research,2009,21(4):616-618.
[23]JIANG HL,CHEN J,JIN XJ,et al.Sequiterpenoids alantolactone analogues and secoguaiene from the roots of Inula helenium[J].Tetrahedron,2011,67(47):9193-9198.
[24]ZHANG L,WANG MY,LI XB,et al.Extraction and purification of alantolactone and isoalantol-actone from root of Inula helenium[J].Natural Product Research,2015,27(1):126-133.
[25]胡晓炜,徐爱仁.三种木香的气相色谱法鉴别与系统聚类分析[J].中国现代应用药学,2005(3):249-252.
[26]李婷婷,黄晓婧,王欣,等.冠心苏合丸的GC特征图谱研究[J].华西药学杂志,2021,36(5):535-538.
[27]李瑾,卜雕雕,王媚,等.固相萃取净化-气相色谱质谱联用法快速测定金银花软胶囊浸膏中的农药残留[J].现代中医药,2017,37(3):75-78.
[28]廖然,谢伟.气相色谱法测定解毒扶正胶囊中甲基正壬酮含量[J].现代中医药,2015,35(4):59-60.
备注/Memo
- 备注/Memo:
-
基金项目:国家自然科学基金地区项目(81460651)
更新日期/Last Update:
2024-05-20