[1]宋健 袁敏惠 刘争辉 崔冰慧 褚宇鹏黄毓娟 惠建萍沈舒文 于勇 孟凯强.基于“毒瘀交阻”探讨金果胃康胶囊对PLGC大鼠Wnt/Lgr5通路调控的影响[J].现代中医药,2021,(04):027-31.[doi:10.13424/j.cnki.mtcm.2021.04.006]
　SONG JianYUAN Minhui LIU Zhenghui CUI Binghui CHU Yupeng HUANG Yujuan HUI Jianping SHEN Shuwen YU Yong MENG Kaiqiang.Effect of Jinguo Weikang Capsule on Wnt/Lgr5 Pathway Regulationof PLGC Rats Based on “Toxin Stasis Combined Obstruction”[J].Modern Traditional Chinese Medicine,2021,(04):027-31.[doi:10.13424/j.cnki.mtcm.2021.04.006]
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基于“毒瘀交阻”探讨金果胃康胶囊对PLGC大鼠Wnt/Lgr5通路调控的影响
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《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年04期
页码:
027-31
栏目:
出版日期:
2023-11-20

文章信息/Info

Title:
Effect of Jinguo Weikang Capsule on Wnt/Lgr5 Pathway Regulationof PLGC Rats Based on “Toxin Stasis Combined Obstruction”
文章编号:
1672-0571(2021)04-0022-05
作者:
宋健1 袁敏惠1 刘争辉1 崔冰慧1 褚宇鹏1黄毓娟1 惠建萍1沈舒文1 于勇2 孟凯强1
1.陕西中医药大学，陕西 咸阳 712046；
2.西安市中医医院，陕西 西安 712021
Author(s):
SONG Jian1YUAN Minhui1 LIU Zhenghui1 CUI Binghui1 CHU Yupeng 1HUANG Yujuan1 HUI Jianping 1 SHEN Shuwen1 YU Yong2 MENG Kaiqiang1
1.Shaanxi University of Traditional Chinese Medicine，Shaanxi Xianyang 712046，China;
2. Xi’an Hospital of Traditional Chinese Medicine,Xi’an 712021,China
关键词:
“毒瘀交阻”理论; 金果胃康胶囊; 胃癌前病变; 大鼠; Wnt/Lgr5信号通路
Keywords:
“Toxin Stasis Combined Obstruction” theory;  Jinguo Weikang Capsule;  Precancerous lesions of gastric cancer;  Rats;  Wnt/Lgr5 signal pathway
分类号:
R285.5
DOI:
10.13424/j.cnki.mtcm.2021.04.006
文献标志码:
A
摘要:
目的 研究基于“毒瘀交阻”理论组方金果胃康胶囊对胃癌前病变大鼠模型Wnt/Lgr5信号通路调控的分子机制。方法 选用SD雄性大鼠随机分为空白组、模型组、金果胃康胶囊（高、中、低剂量）治疗组、维酶素治疗组，我们使用MNNG结合饥饱失常等综合造模法复制PLGC大鼠病理模型，确定模型复制成功，连续灌胃治疗6周后，使用蛋白免疫印迹法检测大鼠胃粘膜组织Wnt/Lgr5信号通路基因及蛋白表达水平。结果 与模型组相比较，金果胃康胶囊治疗组大鼠胃黏膜上皮组织中Wnt1mRNA、β-cateninmRNA、Lgr5mRNA表达水平均显著降低，有统计学意义（P<0.01）。结论 金果胃康胶囊能够有效治疗PLGC，降低胃癌发生风险，可能与通过调控Wnt/Lgr5信号通路，降低大鼠胃黏膜组织Wnt1、β-catenin、Lgr5的蛋白表达及基因转录相关。
Abstract:
Objective To study the molecular mechanism of the regulation of Wnt/Lgr5 signaling pathway in the model of gastric cancer rats based on the theory of “Toxin Stasis Combined Obstruction”.Methods SD male rats were randomly divided into blank group，model group，Jingguo Weikang Capsule (high，medium and low dose) treatment group and vitamin enzyme treatment group. We used MNNG combined with the comprehensive model making method of starvation and satiety to copy the pathological model of PLGC rats，and confirmed that the model was successfully copied and treated continuously for 6 weeks，The Wnt/Lgr5 signaling pathway gene and protein expression level in gastric mucosa of rats were detected by Western blot. Results: Compared with the model group，the expression levels of Wnt1 mRNA,β-catenin mRNA，Lgr5mRNA in the gastric mucosa epithelium of the rats in the treatment group was treated with Jinguo Weikang Capsule were significantly reduced (P<0.01).Conclusion Jinguo Weikang Capsule can effectively treat PLGC and reduce the risk of gastric cancer. It may be related to the reduction of Wnt1，β-catenin mRNA，Lgr5mRNA expression and gene transcription in gastric mucosa of rats by regulating Wnt/Lgr5 signaling pathway.

参考文献/References:

［1］王宁,刘硕,杨雷，等.2018全球癌症统计报告解读［J］.肿瘤综合治疗志,2019,5(1):48-53.

［2］Glinka A，Dolde C，Kirsch N，et al.LGR4 and LGR5 are R-spondin receptors mediating Wnt/β-catenin and Wnt/PCP signalling［J］.EMBO Rep，2011，12（10）：1055-1061.?
［3］Ruffner H，Sprunger J，Charlat O，et al.R-Spondin potentiates Wnt/β-catenin signaling through orphan receptors LGR4 and LGR5［J］.PLoS One，2012，7（7）：e40976.
［4］牛磊,郗洪庆,陈凛.Lgr5-Wnt/β-catenin信号通路与胃癌干细胞的研究进展［J］.解放军医学院学报,2014,35(12):1268-1272.
［5］刘伟,潘华峰,赵自明,等.健脾化瘀解毒方调控Wnt/β-catenin/GSK3β通路抑制胃癌前病变大鼠Lgr5~+癌干细胞早期转移机制研究［J］.中华中医药杂志,2017,32(5):1952-1956.
［6］文娜,胡运莲.Lgr5、TFF2在萎缩性胃炎及低级别上皮内瘤变中的表达及临床意义［J］.中国现代医生,2019,57(12):20-23，169.
［7］曾进浩.健脾化瘀解毒方调控Wnt通路介导胃癌前病变大鼠Lgr5+细胞的机制研究［D］.广州：广州中医药大学,2016.
［8］CongH，Yao RY，Sun ZQ，et al.DNA hypermethylation of the vimentin gene inversely correlates with vimetin expression in intestinal and diffuse-type gastric cancer［J］.Oncology letters，2016，11(1):842-848.
［9］叶景阳,朱西杰,王儒,等.复方蜥蜴散不同微粒组合剂对胃癌前病变模型大鼠Wnt信号通路下游C-Myc、Cyclin-D1蛋白表达的影响［J］.时珍国医国药,2016,27(7):1572-1575.
［10］李京伟,姜小艳,陈玉琴,等.Lgr5与Wnt/β-catenin通路在胃癌进程中的作用研究［J］.中国实验诊断学,2019,23(12):2055-2059.
［11］刘栋,王建华,段降龙,等.miR-582-5p通过调控Wnt通路影响胃癌AGS细胞的增殖和耐药［J］.山西医科大学学报,2018,49(5):470-476.
［12］张振华,侯海金,张斌,等.白藜芦醇通过调控Wnt/β-catenin信号通路抑制体外胃癌细胞生长的研究［J］.中华临床医师杂志(电子版),2017,11(15):2087-2092.
?［13］沈舒文,宇文亚,汶明奇,等.金果胃康胶囊治疗胃癌前病变的临床观察［J］.中医药学刊,2005,23(1):73-74,83.
［14］王捷虹,宇文亚,惠建萍.沈舒文教授毒瘀交阻理论辨治胃癌前病变经验［J］.中华中医药杂志,2013,28(7):2034-2036.
［15］惠建萍,沈舒文,刘力,等.从“毒瘀交阻”理论辨治胃癌前病变的思路与方法［J］.世界科学技术(中医药现代化),2012,14(1):1270-1272.
［16］沈舒文,宇文亚,张贞鲁,等.Effect of Jinguo Weikang Capsule(金果胃康胶囊) on Proto-oncogene Expression of Gastric Mucosa in Rats with Gastric Precancerous Lesions［J］.Chinese Journal of Integrative Medicine,2008,(3):212-216.
［17］白苇,沈舒文,王婕红,等.金果胃康胶囊治疗胃黏膜异型增生60例临床观察［J］.吉林中医药,2008,30(4):268-269.
［18］黄毓娟,沈舒文,宋健,等.金果胃康胶囊治疗胃黏膜上皮内瘤变患者的临床研究［J］.中国临床药理学杂志,2020,36(5):486-489.
［19］黄毓娟,沈舒文,惠建平,等.人乳头瘤病毒16 E6蛋白通过Wnt/β-catenin通路促进食管癌Eca109细胞增殖、迁移的实验研究［J］.病毒学报,2020,36(2):255-261.

备注/Memo

备注/Memo:
基金项目：陕西省教育厅专项科学研究计划项目（18JK0225）；国家级大学生创新创业训练计划项目（201910716024）
更新日期/Last Update: 2021-08-02