[1]惠朋利,朱社教,王成,等.基于生物信息学探讨葛根芩连汤治疗溃疡性结肠炎的可行性[J].现代中医药,2024,(01):095-101.[doi:10.13424/j.cnki.mtcm.2024.01.020]
 HUI Pengli,ZHU SheJiao,WANG Cheng,et al.Exploring the Feasibility of Gegen Qinlian Decoction in Treating Ulcerative Colitis Based on Bioinformatics[J].Modern Traditional Chinese Medicine,2024,(01):095-101.[doi:10.13424/j.cnki.mtcm.2024.01.020]
点击复制

基于生物信息学探讨葛根芩连汤治疗溃疡性结肠炎的可行性()
分享到:

《现代中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
095-101
栏目:
出版日期:
2024-01-20

文章信息/Info

Title:
Exploring the Feasibility of Gegen Qinlian Decoction in Treating Ulcerative Colitis Based on Bioinformatics
文章编号:
1672-0571(2024)01-0095-07
作者:
惠朋利朱社教王成谢红沈维艳王谧张涛邹崇伟高宏君曾红梅
安康市中医医院,陕西 安康 710000
Author(s):
HUI PengliZHU SheJiaoWANG ChengXIE HongSHEN Weiyan WANG MiZHANG TaoZOU ChongweiGAO HongjunZENG Hongmei
Ankang Traditional Chinese Medicine Hospital,Shaanxi Ankang 710000,China
关键词:
关键词:葛根芩连汤湿热证溃疡性结肠炎中医网络药理学作用机制
Keywords:
Key words:Gegen Qinlian decoctionDamp heat syndromeUlcerative colitisTraditional Chinese medicineNetwork pharmacologyMechanism of action
分类号:
R285.5
DOI:
10.13424/j.cnki.mtcm.2024.01.020
文献标志码:
A
摘要:
摘要:目的 基于生物信息学探讨葛根芩连汤(GGQL)治疗溃疡性结肠炎(UC)的作用靶点及作用机制。方法 检索TCMSP数据库得到方剂中各药物的有效成分及靶基因;再检索OMIM数据库、DisGeNET数据库获得疾病的基因,将药物基因与疾病基因取交集得到核心基因;采用STRING数据库构建基因功能关联网络;采用DAVID数据库进行GO富集分析和通路富集分析。结果 共获得有效成分278种,靶点410个,疾病基因914个;将药物基因与疾病基因取交集共得到113个核心基因,然后采用STRING数据库构建基因功能关联网络,将网络信息数据导入Cytoscape软件进行拓扑分析发现IL6、STAT3、IL1B、VEGFA、MAPK1、EGF、PTGS2、EGFR、ICAM1、MMP9等靶点度值较高,可能是核心靶点;使用DAVID数据库对靶点进行GO富集分析共得到16个富集的生物过程聚类,通路富集分析共获得26个富集的通路聚类,其中与炎症密切相关并且联系靶点较多的通路有TNF信号通路和炎症性肠病信号通路。结论 研究初步得到了GGQL治疗UC的作用靶点以及作用通路,结合已发表的相关研究认为GGQL可能通过TNF信号通路发挥作用。
Abstract:
Abstract:Objective Based on bioinformatics,to explore the target and mechanism of action of Gegen Qinlian decoction (GGQL) in the treatment of ulcerative colitis (UC).Methods Retrieve the TCMSP database to obtain the effective ingredients and target genes of each drug in the formula;Retrieve the OMIM database and DisGeNET database to obtain disease genes,and intersect drug genes with disease genes to obtain core genes;Then,a gene function association network was constructed using the STRING database;Finally,the DAVID database was used for GO enrichment analysis and pathway enrichment analysis.Results As a result,a total of 278 effective ingredients,410 targets,and 914 disease genes were obtained;A total of 113 core genes were obtained by intersecting drug genes with disease genes.Then,a gene functional association network was constructed using the STRING database.The network information data was imported into Cytoscape software for topology analysis,and it was found that IL6,STAT3,IL1B,VEGFA,MAPK1,EGF,PTGS2,EGFR,ICAM1,MMP9 and other target values were high,which may be core targets;Using the DAVID database for GO enrichment analysis of targets,a total of 16 enriched biological process clusters were obtained,and 26 enriched pathway clusters were obtained through pathway enrichment analysis.Among them,the pathways closely related to inflammation and closely related to targets include the TNF signaling pathway and inflammatory bowel disease signaling pathway.Conclusion The preliminary study has identified the target and pathway of GGQL in the treatment of UC,and combined with relevant published studies,it is believed that GGQL may play a role through the TNF signaling pathway.

参考文献/References:

[1]张晓峰,李萌.针灸联合小建中汤治疗脾胃虚寒型消化性溃疡的临床效果观察[J].临床合理用药杂志,2020,13(14):70-71.
[2]Deng XC,Wang YD,Tian L,et al.Anneslea fragrans Wall.ameliorates ulcerative colitis via inhibiting NF-κB and MAPK activation and mediating intestinal barrier integrity[J].Journal of Ethnopharmacology,2021,278:114304.
[3]高东,邵喜风.葛根芩连汤治疗溃疡性结肠炎的疗效及对患者炎性反应和免疫功能的影响[J].解放军医药杂志,2018,30(10):89-92.
[4]Rubalcava NS,Gadepalli SK,Criss CN,et al.Single-stage restorative proctocolectomy for ulcerative colitis in pediatric patients:a safe alternative[J].Pediatric Surgery International,2021,37(10):1453-1459.
[5]季芳,鞠宝兆,高文艳.基于浊毒理论治疗溃疡性结肠炎的疗效及对血清脑肠肽、炎症因子的影响[J].海南医学院学报,2019,25(20):1557-1561.
[6]Stewart MJ,Bessissow T,Gregor J,et al.Subcutaneously administered anti-TNFs for the treatment of ulcerative colitis:a retrospective,propensity score-matched,US health claims analysis[J].Advances in Therapy,2021,38(7):4115-4129.
[7]杨银利,吴婷,鱼富丽,等.中医综合治疗溃疡性结肠炎老年患者的临床疗效分析[J].现代中医药,2020,40(3):73-75.
[8]王传颂.葛根芩连汤联合柳氮磺胺吡啶对溃疡性结肠炎患者炎症反应的影响[J].光明中医,2018,33(10):1465-1467.
[9]张玲.葛根芩连五炭汤治疗溃疡性结肠炎的疗效观察[J].中医临床研究,2020,12(23):54-56.
[10]王萍丽.芍药汤合葛根芩连汤治疗溃疡性结肠炎(肠腑湿热证)临床疗效观察[J].四川中医,2020,38(1):101-103.
[11]袁敏惠,马小兵,杜晓泉.张锡纯治痢方对辨治溃疡性结肠炎的启示[J].陕西中医药大学学报,2018,41(6):113-115.
[12]刘文,王钰乐,卿勇军,等.葛根芩连汤治疗溃疡性结肠炎谱效关系研究[J].中华中医药杂志,2018,33(9):4093-4097.
[13]刘启鸿,黄文彬,骆云丰,等.溃疡性结肠炎当重湿热[J].陕西中医药大学学报,2018,41(6):13-16.
[14]龚文亮.葛根芩连汤联合益生菌治疗溃疡性结肠炎的疗效及睡眠状态的影响[J].世界睡眠医学杂志,2019,6(7):906-907.
[15]吴东升,曹晖,张彧,等.芍药汤通过抑制HIF-1α调节Th17/Treg平衡治疗溃疡性结肠炎[J].中国实验方剂学杂志,2021,27(16):9-15.
[16]马涛.溃疡性结肠炎患者肠道菌群紊乱与TLR/NK-kB、炎症介质表达的关系[J].海南医学院学报,2017,23(14):1903-1906.
[17]张宁,周晓丽,刘帅.加味葛根芩连汤联合常规治疗对重度湿热型溃疡性结肠炎患者的临床疗效[J].中成药,2020,42(2):351-355.
[18]赵益,赖小东,叶争荣,等.葛根芩连汤对溃疡性结肠炎模型大鼠抗氧化及抗炎的作用机制[J].中华中医药杂志,2016,31(5):1741-1745.
[19]黄雪雪,陈莉,余丽双.黄芩成分分析及药理作用研究进展[J].贵州中医药大学学报,2020,42(2):79-82,90.
[20]王会录,王孝郎.黄芩汤加味辅助美沙拉嗪治疗溃疡性结肠炎39例[J].现代中医药,2019,39(3):56-58.
[21]盖晓红,刘素香,任涛,等.黄连的化学成分及药理作用研究进展[J].中草药,2018,49(20):4919-4927.
[22]秦凯健,崔灿,黄信松,等.葛根芩连汤加减方治疗溃疡性结肠炎的meta分析[J].国际中医中药杂志,2019,41(1):99-103.
[23]李冀,李想,曹明明,等.甘草药理作用及药对配伍比例研究进展[J].上海中医药杂志,2019,53(7):83-87.
[24]杨辉,王翼洲.热瘀散对溃疡性结肠炎胃肠湿热型大鼠模型MDA、SOD、IL-6的影响[J].广西中医药,2007,30(4):54-55.
[25]Dou B,Hu WJ,Song MK,et al.Anti-inflammation of Erianin in dextran sulphate sodium-induced ulcerative colitis mice model via collaborative regulation of TLR4 and STAT3[J].Chemico-Biological Interactions,2020,324:109089.
[26]Mitsialis V,et al.382-high dimensional immune phenotyping using mass cytometry reveals unique IL1B signatures in peripheral blood and GI tissue of ulcerative colitis,Crohn’s disease,and IL10R deficient patients[J].Gastroenterology,2018,154(6):S-90.
[27]刘永升,魏梅,宋春梅,等.当归多糖通过上调VEGF抑制细胞凋亡[J].基因组学与应用生物学,2020,39(3):1307-1313.
[28]谭俊杰,吴文雪,廖艳花,等.二苯乙烯苷对冈田酸诱导的AD细胞模型磷酸激酶MAPK1、JNK、CK1、PKA的影响[J].广西中医药,2019,42(6):51-54.
[29]范普雨,王宁.白头翁汤联合透灸对湿热型溃疡性结肠炎患者炎症细胞因子和免疫功能的影响[J].中医学报,2021,36(6):1335-1338.
[30]Rawat C,Kutum R,Kukal S,et al.Downregulation of peripheral PTGS2/COX-2 in response to valproate treatment in patients with epilepsy[J].Scientific Reports,2020,10(1):2546.
[31]焦俊霞,叶晓霞.EGFR、P53、Ki-67在结直肠癌中表达及临床病理学意义[J].中国老年学杂志,2020,40(10):2065-2068.
[32]张海林,卢晓君,冯流畅,等.慢性肾脏病湿热型体质易感人群ICAM1基因和IL-10基因启动子多态性研究[J].西部中医药,2018,31(12):1-4.
[33]Liu SX,Li QD,Liu FZ,et al.Uncovering the mechanism of Curcuma in the treatment of ulcerative colitis based on network pharmacology,molecular docking technology,and experiment verification[J].Evidence-Based Complementary and Alternative Medicine:ECAM,2021,2021:6629761.
[34]钟兴腾,安耀荣,朱向东,等.以《周易·损卦》象数思维解析论治溃疡性结肠炎[J].陕西中医药大学学报,2020,43(5):40-42,59.
[35]巩子汉,张玲,刘丽梅,等.探讨四神丸、白头翁汤、连理汤对溃疡性结肠炎大鼠的影响[J].中国实验方剂学杂志,2021,27(16):1-8.
[36]闫曙光,许小凡,李倩,等.基于Notch/NF-κB/NLRP3信号通路的巨噬细胞活化与溃疡性结肠炎的研究进展[J].中南大学学报(医学版),2019,44(10):1174-1178.
[37]孙娟,葛雨竹,李姿慧,等.参苓白术散通过TLR4/NF-κB 通路对溃疡性结肠炎小鼠的抑制作用研究[J].中国免疫学杂志,2020,36(3):294-298,304.
[38]Ding XF,Yang DR,Xia LQ,et al.Correction to:targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating acrophages with alteration of the TIMP-1/MMP2/MMP9 signals[J].Molecular Cancer,2020,19(1):97.
[39]高威,张昱,张伟,等.DSS模型小鼠结肠组织变化及葛根芩连汤干预的实验研究[J].时珍国医国药,2019,30(2):510-512.
[40]张天涵,沈洪.基于IL-6/JAK2/STAT3信号通路中药抗溃疡性结肠炎研究进展[J].环球中医药,2019,12(10):1600-1605.
[41]Li QM,Cui Y,Xu BC,et al.Main active components of Jiawei Gegen Qinlian Decoction protects against ulcerative colitis under different dietary environments in a gut microbiota-dependent manner[J].Pharmacological Research,2021,170:105694.

相似文献/References:

[1]焦靖淇,高天舒.高天舒教授从“浊邪害清”理论论治糖尿病认知功能障碍[J].现代中医药,2023,(01):071.[doi:10.13424/j.cnki.mtcm.2023.01.013]
[2]赵亚萍 杨丽霞 甘德成 朱向东 梁永林.基于肠道菌群探讨葛根芩连汤治疗湿热型2型糖尿病研究进展*[J].现代中医药,2023,(02):001.[doi:10.13424/j.cnki.mtcm.2023.02.001]
 ZHAO Yaping YANG Lixia GAN Decheng ZHU Xiangdong LIANG Yonglin.Research Progress of Gegen Qinlian Decoction in Treatment ofDamp Heat Type 2 Diabetes Based on Intestinal Flora[J].Modern Traditional Chinese Medicine,2023,(01):001.[doi:10.13424/j.cnki.mtcm.2023.02.001]

备注/Memo

备注/Memo:
基金项目:陕西省科技厅项目(2019SF-099
更新日期/Last Update: 2024-01-27